Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,129,796	G→A	S12F (TCC→TTC)	flgN ←	flagellar biosynthesis protein FlgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,129,796	0	G	A	100.0%	62.9 / NA	22	S12F (TCC→TTC)	flgN	flagellar biosynthesis protein FlgN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/7);  total (15/7)

CGTTATGTTGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCG  >  NC_000913/1129551‑1130040                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     cGTTATGTTGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATc                                                                                                                                                                                                                                                 >  1:93760/1‑251 (MQ=255)    tATGTTGATTCATTTGGCGTAGTTGCTGCGTTGTCACAGTAATTTCCTGCCAGCGTTGGCTAATATCAACGCTATTTGCTGTATTGGGTACTGTCCTGCGTAACTGTTCGAGGTAATCCATCGTCGCCAGCAGTGAGCTTTTTTGTTCGGTAAGCCATTGCAACTTGCTGCCGTTGAGCTGCCCCATAGAGAGATGTTTCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTg                                                                                                                                                                                                                                               <  1:262559/250‑1 (MQ=255)              cATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATTCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATATAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGAtct                                                                                                                                                                                                                                    <  1:28495/251‑1 (MQ=255)                                                 ccTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTTCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGGGTCATACGATCAGTTACTCTGCa                                                                                                                                                                                                  >  2:47536/1‑250 (MQ=255)                                                  cTGCCAGCGTTGACTAATATCACCGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGCGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCa                                                                                                                                                                                                  >  2:26694/1‑249 (MQ=255)                                                               cTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCtt                                                                                                                                                                                   >  1:232030/1‑251 (MQ=255)                                                                      caatgCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTGCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACTATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGa                                                                                                                                                                             <  2:10490/246‑1 (MQ=255)                                                                                                ttCCTGCGTAAATGTTCGAGGTAATCCAGCGTCGCCAGCAGTGCGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCACCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGgtgt                                                                                                                                                  >  2:27838/1‑251 (MQ=255)                                                                                                      cGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCCATTTTGCCGGTGTCCAttt                                                                                                                                            >  2:158849/1‑251 (MQ=255)                                                                                                        tAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCAttttt                                                                                                                                          >  2:48529/1‑251 (MQ=255)                                                                                                          aCTGTTCGAGGTAATCCATCGTCGCCAGCAGTGAGCTTGTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCATTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAg                                                                                                                                        <  1:50204/251‑1 (MQ=255)                                                                                                            tGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGtt                                                                                                                                      >  2:163209/1‑251 (MQ=255)                                                                                                                                cGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGt                                                                                                                  >  1:129390/1‑251 (MQ=255)                                                                                                                                        gTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGc                                                                                                          >  1:138131/1‑251 (MQ=255)                                                                                                                                                             tCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGat                                                                                     >  1:97383/1‑251 (MQ=255)                                                                                                                                                                         ggCTGCCGTTGATCTGCCCCCTAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGCCGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGcc                                                                         >  1:230218/1‑251 (MQ=255)                                                                                                                                                                                                                  tGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGCTTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCacac                                >  1:223844/1‑251 (MQ=255)                                                                                                                                                                                                                       cATTCCCGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACActggtg                           <  2:97383/251‑1 (MQ=255)                                                                                                                                                                                                                           gccGTTTTGAGATCGTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGAGCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCTTCGCTTAACGTCACActggtgctgg                       <  2:138131/250‑1 (MQ=255)                                                                                                                                                                                                                                         gTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCActgct                                                                            >  1:78801/1‑184 (MQ=255)                                                                                                                                                                                                                                         gTTAAGCACAGCGAACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCActgct                                                                            <  2:78801/184‑1 (MQ=255)                                                                                                                                                                                                                                                acaGCGAACATCTGGTCGCGGATCTCTGAAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCACGCTTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGGGGAGGCGGATttttttttcg  >  2:129111/1‑247 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     CGTTATGTTGATTCATTTGGCGTAGTTGCTGCGTTTTCACAGTAATTTCCTGCCAGCGTTGACTAATATCAACGCTATTTGCTGTATTGGGTTCTTTCCTGCGTAACTGTTCGAGGTAATCCAGCGTCGCCAGCAGTGAGCTTTTTTGTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGTCTTGCTGCGCTTCGTTGATCAGCGCATCGGCAATTTTGCCGGTGTCCATTTTTAGTTCACCGTTACGAATCGCCAGTTTTAACGCTTCGACACGTTCAAGATTGATATCACTGCTGCCGGGTTGCATCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCG  >  NC_000913/1129551‑1130040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑