| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,126,316 | G→A | E54K (GAA→AAA) | yceH → | DUF480 domain‑containing protein YceH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,126,316 | 0 | G | A | 95.7% | 63.5 / ‑3.5 | 23 | coding (160/648 nt) | yceH | DUF480 domain‑containing protein YceH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (11/11); minor base . (0/1); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
AATGGCGCGATCACGTACTGACGGCATTAACTACCCCAGACTGGACGCCCGGCCGCTAAGGAGAATCTCATGAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCGAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGG > NC_000913/1126088‑1126550 | aagggCGCGATCACGTACTGACGGCATTAACTACCCCAGACTGGACGCCCGGCCGCTAAGGAGAATCTCATGAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAATCTGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGGGTAGTCACGGCGTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGGGAATCCAAATT‑GCAGGAACAGCTGGATaa < 2:261918/248‑1 (MQ=255) cgcgATCACGTACTGACGGCATTAACTACCCCAGACTGGACGCCCGGCCGCTAAGGAGAATCTCATGAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTgg > 1:254395/1‑251 (MQ=255) gATCACGTACTGACGGCATTAACTACCCCAGACTGGACGCCCGGCCGCTAAGGAGAATCTCATGAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCa < 1:47170/251‑1 (MQ=255) cactAACTACCCCAGCCTGGACGCCCGGCCGCTAAGGAGAATCTCATGAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATGCGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCGCAGTCAATGGTGTAGTCACGGCCTGTAATCATAAAACGAACCGTGAACCTGGCATGAATCTGATTGAATCC‑AAGTTGCAGGAAGAGCTGGATGATCTCTTCAAACGTGATTTTCTACg < 2:30599/248‑1 (MQ=255) aCTGGACGCCCGGCCGCTAAGGAGAATCTCATGAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGttt > 2:102357/1‑251 (MQ=255) ctcATGAAATATCAACTTCCTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGc > 2:177705/1‑250 (MQ=255) ctcATGAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCAGGGCCTGTAATCAGAAAACGAACCGTGAACCGGCCATGATTCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATAGGGTCACCAAATATGAGc > 1:257212/1‑250 (MQ=255) gAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATTGGGTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGGGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTTGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGtt < 1:268461/251‑1 (MQ=255) tACTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGGGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCa < 2:146671/250‑1 (MQ=255) aaGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGAt > 1:219103/1‑251 (MQ=255) gTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCATGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATATGAAACt < 2:3914/251‑1 (MQ=255) acccgatataaGTCAATGGTGTAGTCACGGCCTGTAATCAGAAACCGAACCGTGAACCGGTCATGCATCTGAGTGCATCCAAAGC‑GCCCGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATAAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCgtggt > 2:16359/11‑251 (MQ=255) ccGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGc > 2:138596/1‑251 (MQ=255) cGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCTGGTCACCAAATATGATCAACGGTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgc < 1:19933/251‑1 (MQ=255) ggCCTGTAATCAGAAAACGAACCGTGAACCCGTCATTAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTGTTAATTCAGAATTTGGCGATCTGAAACGGAGCTCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgcc < 2:257212/251‑1 (MQ=255) ccTGTAATCAGCAAAAGAACCGTGAAAAGGTCATGAATCTGAGTGAATCCAAAGA‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGCGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgccgc > 2:110511/1‑251 (MQ=255) aactgAAAACGAACCGTGAACCGGTCATGAATCTGAGTTAATCCAAAGG‑GCAGGAACAGCTGGATAAGCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGccgcgtcgcg < 2:148095/247‑1 (MQ=255) cAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑TCAGGAACAGCTGGATAATCTGGTCAAACGGCATTATCTACGCACAGTGAGCGGTTTTGGTAAGCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATGGCGTGGTGCCCAGACGCCAGGAGAACTGCGCAGCCGCGTcgcgcg < 2:263681/251‑1 (MQ=255) aaCGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCATGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCGCCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACGGAGCGCAGCGGAAGTGGCGTTAATCCCCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgccgcgtc < 1:36017/241‑3 (MQ=255) aaCGAAACGTGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGAACCGCTGGATCATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGAAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGAGCCCAGACGCCAGGTGAACTGCGCAgccgcgtc > 2:36017/1‑239 (MQ=255) gAACCGAGAACCGGTCATGAATCTGAGTGAATCCAAAGT‑GCAGGCACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATg > 2:127969/1‑251 (MQ=255) aTGAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGc > 1:116381/1‑251 (MQ=255) gAATCTGAGTGAATCCAAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCgg < 1:253704/251‑1 (MQ=255) | AATGGCGCGATCACGTACTGACGGCATTAACTACCCCAGACTGGACGCCCGGCCGCTAAGGAGAATCTCATGAAATATCAACTTACTGCCCTTGAAGCTCGCGTGATTGGTTGCTTGCTGGAAAAGCAGGTGACCACGCCGGAGCAATACCCGCTCTCAGTCAATGGTGTAGTCACGGCCTGTAATCAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCGAAGT‑GCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGG > NC_000913/1126088‑1126550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |