Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,762	G→A	V282V (GTC→GTT)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,762	0	G	A	100.0%	84.1 / NA	28	V282V (GTC→GTT)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)

ATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGT  >  NC_000913/1121515‑1121988                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   atttatACAGGTAAACTAACCTAATGACAACAAGAAGCTCCGTTTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAACGCATTCATTTCTTCAAAAACg                                                                                                                                                                                                                                 >  2:183202/1‑251 (MQ=255)  tttatACAGGTACAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACgg                                                                                                                                                                                                                                >  1:81460/1‑251 (MQ=255)             aaaaTAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTg                                                                                                                                                                                                                     <  1:77521/251‑1 (MQ=255)                  aaCCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAAAGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCCCGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACATAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGccc                                                                                                                                                                                                                  >  2:176595/1‑249 (MQ=255)                   aCCTAATGACAACATGAAGCTCCGATTTTTATTGTTTAACGGACCAGCGTCCCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCATCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGAACGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTATCTGCCCAgc                                                                                                                                                                                                               >  2:85351/1‑251 (MQ=255)                                       tACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAACAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAagc                                                                                                                                                                                            >  2:142712/1‑250 (MQ=255)                                                      ttAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGc                                                                                                                                                                             >  1:253429/1‑250 (MQ=255)                                                               cAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGctctc                                                                                                                                                                   >  2:67600/1‑251 (MQ=255)                                                                                           gCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCTGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTTCATGTGGCGCAGaa                                                                                                                                       <  1:183202/251‑1 (MQ=255)                                                                                              ccAGCGTGTCATCAGTCAGGGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATcc                                                                                                                                    >  1:21936/1‑251 (MQ=255)                                                                                                 gCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGt                                                                                                                                  >  1:143284/1‑250 (MQ=255)                                                                                                  cGTGTCATCAGTCAGGGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGGTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAGCTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTGTGCGATGACGTGCATGTGGCTCATAATTCGTAc                                                                                                                                <  2:196292/251‑1 (MQ=255)                                                                                                     gtCATCAGTCAGTGCGATGCTTTCAGCAACCTGGTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCg                                                                                                                              <  1:155161/250‑1 (MQ=255)                                                                                                         tcagtcagTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTAc                                                                                                                                >  1:86160/1‑244 (MQ=255)                                                                                                         tcagtcagTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTAc                                                                                                                                <  2:86160/244‑1 (MQ=255)                                                                                                                     agtcTTTAAGCAACCTGTTGCGCTTCACGTACCAGTTAGATGAAGGTGTCGTTGACCGGCAACCCATAGAACTGCTGGCCGTTTACAGATCAGAAGGCTTAAAAGTGCTGCAAAGCAGTCATTTGTTCAAAAACGGTAGCGTAACTGCGCAGCGCGGTTGGGGCGTTGAAGCAGCGCGCGCAGTGGCAAGTGATCTCTTTGCGATGACTTACATGGGGCGGAGAATCCGGACGAAGGAATGATCGATTaaa                                                                                                             <  2:238264/248‑1 (MQ=255)                                                                                                                                aaCCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCg                                                                                                   >  2:99962/1‑250 (MQ=255)                                                                                                                                           ctTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGTTTAAAACCGCTGGCGACCAGTTCACGc                                                                                       >  2:206384/1‑251 (MQ=255)                                                                                                                                                    ccAGTTCGATGAATGGGTCGTTGACCGGCAACCTATAGAACTGCGGGCCGTTTACAGAAAAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGGGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGccggt                                                                              <  2:81460/251‑4 (MQ=255)                                                                                                                                                                      cGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTg                                                            <  1:142712/251‑1 (MQ=255)                                                                                                                                                                          gACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAgg                                                         >  1:32583/1‑250 (MQ=255)                                                                                                                                                                            ccGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATg                                                      <  1:99962/251‑1 (MQ=255)                                                                                                                                                                               gCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGt                                                   <  1:67600/251‑1 (MQ=255)                                                                                                                                                                                                       ttACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGttt                                                             <  2:60782/217‑1 (MQ=255)                                                                                                                                                                                                       ttACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGttt                                                             >  1:60782/1‑217 (MQ=255)                                                                                                                                                                                                                                ctgcAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGt  >  1:218407/1‑251 (MQ=255)                                                                                                                                                                                                                                      aGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATCCAGGTGCg                                   >  2:137441/1‑212 (MQ=255)                                                                                                                                                                                                                                      aGCATTCATTTCTTCAAAAACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCg                                   <  1:137441/212‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   ATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGT  >  NC_000913/1121515‑1121988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑