Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,547	G→A	intergenic (‑60/+14)	dinI ← / ← pyrC	DNA damage‑inducible protein I/dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,547	0	G	A	94.1%	42.9 / ‑5.7	17	intergenic (‑60/+14)	dinI/pyrC	DNA damage‑inducible protein I/dihydroorotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (8/8);  minor base T (1/0);  total (9/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GTTTATCTTCTTTTGTCGCGCCAATAACCGATAAATTATTCGCTGCGGCATAACGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGC  >  NC_000913/1121307‑1121783                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             gTTTATCGTCTTTTTTCGCGCCAATAACCGATAAATTAGTCGCTGCGGCATAACGTTCCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCTCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGATGCTAc                                                                                                                                                                                                                                      <  2:258302/249‑1 (MQ=255)               gTCGCGCCAATAACCGATAAATTATTCGCTGCGGCATAACGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTaa                                                                                                                                                                                                                      <  2:5778/251‑1 (MQ=255)                                                          ccGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCgt                                                                                                                                                                           <  1:110404/251‑1 (MQ=255)                                                              aaCGTGGCCTTCAGTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGACAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGtca                                                                                                                                                                       <  2:2114/250‑1 (MQ=255)                                                                cGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGtcatc                                                                                                                                                                     <  1:219460/251‑1 (MQ=255)                                                                                           aCTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCCGCTGGCAATGGGGAAGTTTTCGCTATGGTGGCTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGGTATTTATACAGGTAAAATAACCTAATGACAACAAAAAGCTACGAt                                                                                                                                                                                                                                   >  1:4007/1‑162 (MQ=255)                                                                                           aCTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGAt                                                                                                                                                                                                                                   <  2:4007/162‑1 (MQ=255)                                                                                                                                                         gTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCATCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGc                                                                            <  1:28362/251‑1 (MQ=255)                                                                                                                                                                 tATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTCATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTac                                                                     >  2:147714/1‑250 (MQ=255)                                                                                                                                                                                                  ttGAATATACTGGTTATTTATACAGGTAAAATAAACTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGcattc                                   >  1:105336/1‑251 (MQ=255)                                                                                                                                                                                                        ctaCTGGTTCTTTATACAGGCAAAATAACCTAATGAAACCAAGAAGCTACGATTTTTATTGTTTAACGGACCCGCGTACCGATTCCACGGCGAGGAATCGCCCCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGCTGAATGTGTCGTTGACCCGCCACCAATAGAACTGCGGGCAGTTTACAGAACAGAATGCTTCAAAGTGCTGCCCAGCATTCAtttat                             >  2:255944/2‑249 (MQ=255)                                                                                                                                                                                                        ataCTGGTTATTAATACAGGGAAAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCACCGGCGAGGAATGGCACAAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGATCTTCACTTACCCGTTCGATGAATGTGTAGTTGCCCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGCATGCTTCAAAGTGCTGCAAACCATTAATttct                             >  2:171127/1‑251 (MQ=255)                                                                                                                                                                                                                 atttatACAGGTAAACTAACCTAATGACAACAAGAAGCTCCGTTTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAACGCATTCATTTCTTCAAAAACg                    >  2:183202/1‑251 (MQ=255)                                                                                                                                                                                                                  tttatACAGGTACAATAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACgg                   >  1:81460/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaaTAACCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTg        <  1:77521/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaCCTAATGACAACAAGAAGCTACGATTTTTATTGTTTAAAGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCCCGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACATAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTAACTGccc     >  2:176595/1‑249 (MQ=255)                                                                                                                                                                                                                                   aCCTAATGACAACATGAAGCTCCGATTTTTATTGTTTAACGGACCAGCGTCCCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCATCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGAACGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAAACGGTAGCGTATCTGCCCAgc  >  2:85351/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             GTTTATCTTCTTTTGTCGCGCCAATAACCGATAAATTATTCGCTGCGGCATAACGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGC  >  NC_000913/1121307‑1121783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑