| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,120,111 | G→A | A159V (GCG→GTG) | solA ← | N‑methyl‑L‑tryptophan oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,120,111 | 0 | G | A | 95.7% | 61.5 / NA | 23 | A159V (GCG→GTG) | solA | N‑methyl‑L‑tryptophan oxidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/10); minor base C (0/1); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCT > NC_000913/1119871‑1120347 | gTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATCATCACCGTCAGCCGCTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGAt > 2:46750/1‑251 (MQ=255) gAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGACTTGCGGTGACCGTGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCATTTGGATCCAGGTTTTa > 2:78538/1‑250 (MQ=255) ccATCGGCCTGATACCAGGCAAATTCTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCATTTCGCTGCGCAAAAAAcc < 2:185415/251‑1 (MQ=255) cGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTTACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTGCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGa < 1:180925/250‑1 (MQ=255) aCTTTGCGTACGGGCTGGACAGGCAGCCCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCCGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCTCAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGAt < 1:206721/251‑1 (MQ=255) gTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTg < 2:19360/251‑1 (MQ=255) gctgattccacatttCCCGAGCAGACAATCGCTTTTTTCGCCTGATACTCCCCGTCAGCCGTTTCAATCGTTACTCCATCCTCGTCATGACGCATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAACAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGTCCAGCTGGCCAt > 2:62650/14‑251 (MQ=255) tCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCAt > 1:56524/1‑251 (MQ=255) tCCCGCGCAGACAATCGCTTTTTTCGCCTGAAAATCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGCGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAACAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGACCATAATCCCTTGCGCAt > 1:198768/1‑251 (MQ=255) ccGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGAAGAAGTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCg < 2:58861/251‑1 (MQ=255) cGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGt < 1:78538/251‑1 (MQ=255) aTACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGACATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGtt > 2:85447/1‑251 (MQ=255) aGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTggg > 1:93690/1‑251 (MQ=255) ccATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTAttt > 1:144960/1‑160 (MQ=255) ccATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCGTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTAttt < 2:144960/160‑1 (MQ=255) cGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATc < 1:85447/250‑1 (MQ=255) cATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCaac < 1:62650/250‑3 (MQ=255) aTGACGAATTGCGGTGAACGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGCGTTGCCATTGTTCGTCGCTGTGGGCGACGTTGGCGAGACATGTGGAATCAGCCg > 1:197069/1‑251 (MQ=255) gACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATg > 2:91318/1‑250 (MQ=255) gAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTGCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTa < 2:220679/251‑1 (MQ=255) accagtgcgctccgaccGCTTCCCTCCCCAATTTGATTCAGGTTTTAACCGCCAGGTCGCTGCCCCAAAAACCTGAATCAGTTTCAAAAAAGCCGATGTAGTTTTCCCGGACGCGTATTTCCCGGCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACTGTGATTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAAGGTGGAATCAGCCTGGCCAAGTTTAATGACGCCAGAGTGTACa < 2:175814/236‑1 (MQ=255) gcACAGCCCGCTTACTTCACCAGTTGGATCCAGGTTTTAATCGCCCCTTCGCTGCGCAAAAACCCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGCCGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTACTGACGCCAGAGAGTACCAACATggg > 2:46635/1‑251 (MQ=255) aGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAACAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGCAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAtct > 2:65436/1‑251 (MQ=255) | GTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCT > NC_000913/1119871‑1120347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |