Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,117,707	G→A	G301S (GGT→AGT)	trhO →	tRNA U34 hydroxylase TrhO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,117,707	0	G	A	100.0%	46.1 / NA	17	G301S (GGT→AGT)	trhO	tRNA U34 hydroxylase TrhO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/7);  total (10/7)

CGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAGGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAAT  >  NC_000913/1117484‑1117933                                                                                                                                                                                                                                |                                                                                                                                                                                                                                   cGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGAGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGAGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCga                                                                                                                                                                                                           >  1:229003/1‑249 (MQ=255)                cGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGATGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGc                                                                                                                                                                                          >  2:175265/1‑251 (MQ=255)                        aGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGTTTTGCTGCGAAGAAAGCGCGTTACCGCCagaaga                                                                                                                                                                                   >  2:4225/1‑250 (MQ=255)                                gAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACg                                                                                                                                                                          >  1:2690/1‑251 (MQ=255)                                                                         ttttGACGAGCGGATGGGCGAACGGATATCGGATGAGATTATCGCGCATTGCAACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTtaat                                                                                                                                  <  2:200113/250‑1 (MQ=255)                                                                            tGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGt                                                                                                                              >  1:246618/1‑251 (MQ=255)                                                                                 ggCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGt                                                                                                                         <  2:74803/250‑1 (MQ=255)                                                                                     gATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGAc                                                                                                                     >  1:137095/1‑251 (MQ=255)                                                                                                                 aTCGCGCATTGCCACCAGTGCGGCGCTCCGTGCGACAGCCAGACCAACCGTAAAAATGCTGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCTCGGAAAAATACAAAAGTTGGTGTAGTGAGATTTGCGGTGAAGAAATCGCGTGCCCTGCAGAAGAACAGCGACGTCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACACCACTGTGCATTCCTGa                                                                                         <  1:250828/251‑1 (MQ=255)                                                                                                                       cATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAAc                                                                                   >  1:28912/1‑251 (MQ=255)                                                                                                                       cATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAAc                                                                                   <  1:243486/251‑1 (MQ=255)                                                                                                                        aTTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAAc                                                                                   >  1:117319/1‑250 (MQ=255)                                                                                                                                      ggcgCGCCGGGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCAtt                                                                    <  2:137095/251‑1 (MQ=255)                                                                                                                                                     aGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGcc                                                      >  1:220802/1‑250 (MQ=255)                                                                                                                                                                                            tttATTAAGTGTCCAGTATGCCCGGAAAAATACCAAAGTTGTTGTAGTGAGATTTGCTGCGCAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCACTAAGATCTTTAATACGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCAGGATGCGGGCCATCCGGCAACATTTCACGCTTACTTCTGATGTACGCCTTc              >  2:53840/1‑251 (MQ=255)                                                                                                                                                                                                 tCAGTGTCCAGTATGCGCCGAAAAATACAAAAGTTGGTGTAGTGAGATTTGCTGCGAAGAAAGCGCGATACCGCCAGAAGAACAGCGACGCCGTCTGGCAGGTCGTGAAAATGGCAATAAGATCTTTAATAAGACTCGTGGACGTCTGAATACAACACTGTGCATGCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCTTCCGGTTACATTTCTCGCTTACTTCTGCTGTACGctgttcact          <  2:86976/250‑9 (MQ=255)                                                                                                                                                                                                        ccAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAaataat  <  2:117319/251‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                   CGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAGGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAAT  >  NC_000913/1117484‑1117933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑