Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,115,185	C→T	G102G (GGG→GGA)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,115,185	0	C	T	100.0%	46.8 / NA	17	G102G (GGG→GGA)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/11);  total (6/11)

GAGCACACTGGCGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCA‑GGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGCCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACTGAAGGCGGCACCGGTAAGAAAACAGCCTAGC  >  NC_000913/1114945‑1115431                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       tggCACACTGGCGGTCATAAAGAATCCCGGTGGTAGTCCGTCTCTATCGTCGGGCA‑GGCCGCTAGCCACTTGGCCGAGCAACGCACCACTAACGCCGCCTGTTGAGAGCGTCCCCAGCTCCCAGCCGCTTTTATTACGCGGTACTCGTTTGGCTATAAGAGCATTAGCGTTTGGGACAAATCCGCCACTTAACCCACGAAGCGCCCGCAGGATCAAA‑AACAGCCCGCTATTTTTTTCCATTCCCATCAac                                                                                                                                                                                                                                               <  2:221186/248‑1 (MQ=255)          gacgttaaaaaaGAATACCGGACGTAACCCGTGGCTAGCG‑TGAGCAAGGCCGCCAGCCATTGGGCCGAGCATCGCACCTCTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGGTGGGGGCTAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTGTCGGCCATTCCCATCAACACCATCACg                                                                                                                                                                                                                                      <  2:16578/241‑1 (MQ=255)                   aaaGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCA‑GGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGGGCCAGTCCCATCAACACCATCACGATGCCCATGc                                                                                                                                                                                                                            >  1:137082/1‑251 (MQ=255)                          cccGGACGTAAGCCGTAGCTATCGGCGAGCA‑GGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGc                                                                                                                                                                                                                     <  1:85840/250‑1 (MQ=255)                            cGGACGTAAGCCGTAGCTATCGGCGAGCA‑GGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGc                                                                                                                                                                                                                     >  1:124072/1‑249 (MQ=255)                                   aaGCCGTAGCTATCGGCGAGCA‑GGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTa                                                                                                                                                                                                             <  2:215662/250‑1 (MQ=255)                                                       cA‑GGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGACGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCtt                                                                                                                                                                                        >  2:92213/1‑251 (MQ=255)                                                         gggCCGCCAGCCATTGGGCCGAGCAACGCCCCACTACCGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGccgtt                                                                                                                                                                                       <  2:66380/250‑4 (MQ=255)                                                                                                 cgccTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCCAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGcc                                                                                                                                               >  1:139641/1‑251 (MQ=255)                                                                                                                                     tattaCGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGA‑CAAACACCTGCCAGATATCTTCTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCAAGCGTTTTCGGCCTTTACGGCCGGCGAGACCACCCCAAAACGGTGAGACGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATAcaggcc                                                                                                           >  1:182025/1‑246 (MQ=255)                                                                                                                                                     tgtgGCGATAAGAGAATTAGCGTTGGGGACCAATCCGCCAAGTAACCCAAGAAGCGCCCGAAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGCGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCgg                                                                                           >  2:122738/1‑251 (MQ=255)                                                                                                                                                                       aGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCa                                                                          <  1:50330/250‑1 (MQ=255)                                                                                                                                                                         ggTTGGTGACAAATCCGCCAAGGAACCCAAGAAGCGCCCGCAGGATCAAA‑AACGGCCAGATATTTTGTGCCAGTCCCATCATCACCATCACGATGCCCATGCCGAGGGCAGAGCGTCAGAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATTGCCGAAAATAAAAATGTAATGCTGAAGACAATGCCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAgc                                                                       <  2:7183/250‑1 (MQ=255)                                                                                                                                                                                                aaCCCAAGAGGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGGGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGAACGGTAACGCCAAGCTGCTCAACGTAGAGGGGTaagaa                                                <  2:86088/251‑1 (MQ=255)                                                                                                                                                                                                 aCCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTaagaag                                               <  1:191902/251‑1 (MQ=255)                                                                                                                                                                                                                            aaCTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACTGAAGGCGGCAcc                     <  2:124072/251‑1 (MQ=255)                                                                                                                                                                                                                                               ccAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACTGAAGGCGGCACCGGTAAGAAAACAGCCTAGc  <  1:122738/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GAGCACACTGGCGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCA‑GGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAA‑AACTGCCAGATATTTTGTGCCAGCCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACTGAAGGCGGCACCGGTAAGAAAACAGCCTAGC  >  NC_000913/1114945‑1115431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑