Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,112,421	C→T	S520F (TCT→TTT)	opgH →	osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,112,421	0	C	T	100.0%	79.2 / NA	25	S520F (TCT→TTT)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/7);  total (18/7)

CGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCG  >  NC_000913/1112223‑1112667                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       cgTTGATGCGCCGTGCCGCTTGGCGGGTCTGGCTTGCTTAAGATCTACCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGCTGGTGCCACGGCAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTCTGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTCTGTTCCTCGAGCTCTCTACTGCATTg                                                                                                                                                                                                    >  2:165318/1‑251 (MQ=255)       ggcgcCGTGCAGGTGGGGGGGTCTGTATTGCTTACGACATCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGAGGCGCGAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGCACTTCCGTCTGTTCCTGGTGAAGGGTATGCCCCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTa                                                                                                                                                                                              <  1:2896/250‑1 (MQ=255)         cgcCGTTCGGGTTGGGGGGGTTGGTTTCCTTACGAGCTCCCGGGTTCTTATAAAGAATTGCCGCCTACTTTGTTTGATGAGATAAAAGGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGt                                                                                                                                                                                            <  2:90059/251‑1 (MQ=255)                          ggTCTGGATTGCTTACGATCTCCCGGGTTCTTCTGAAGAATTGCCGCCTAACCTGCTTGATGAGCTAAAACGTGCCCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGCCCGAAc                                                                                                                                                                           >  1:229676/1‑251 (MQ=255)                           gTCTGGATGGCTTACGATCCCCCGGGTTTTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCAAAACCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAAcc                                                                                                                                                                          <  1:60484/251‑1 (MQ=255)                              tGGATCGCTTACGATCTCCAGGGTTCTTATGAAGCATTGCCGCCTAACTTGCTTGATCAGCTAAAACGTGACCGCCGATGGTGCCACGGTACCCTGATGCACTTCCGTCTGTTCCTGGTGACGGGTCTGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGATTTATGTTCCTCGCGCTCTCTACTGCATTGCCGGTCGTGCATGCGTTGACCGAACCGcc                                                                                                                                                                       >  2:107186/1‑250 (MQ=255)                                gATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAAt                                                                                                                                                                     >  1:120717/1‑251 (MQ=255)                                                           agaagaaTTGCCGCCGAACGTACGTGATGAGCTAAAACTGGTCCGCCGATGGTGCCACGGTAACCTGATGAACTGCCGTCTGTTCGTGGGGAAGGGTATGCACCCGGTTCTCCGTTCTGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCTTGCGTTGAGCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTcc                                                                                                                                          <  2:49106/250‑1 (MQ=255)                                                             aagaaTTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTccc                                                                                                                                         >  1:56620/1‑250 (MQ=255)                                                                               tgcttgATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGt                                                                                                                      >  1:15594/1‑251 (MQ=255)                                                                                 cttgATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTcc                                                                                                                    >  1:97473/1‑251 (MQ=255)                                                                                       gactaaaccgtgtcccgccgATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCt                                                                                                              <  2:65181/237‑1 (MQ=255)                                                                                         gCTAAAAAGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTACGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTgg                                                                                                            >  2:266056/1‑251 (MQ=255)                                                                                                 gTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGcc                                                                                                    >  1:224472/1‑250 (MQ=255)                                                                                                          gATGCTGCCACGGTAACCTGATGAACTTACGTCTGTTCCTGGTGAAGGGTATGCACCCGGATCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCACCGTGTGGCAGCAGTGGCGTCCTGCGCTGGCGATTGCCCTTTTTGCt                                                                                           >  2:268355/1‑251 (MQ=255)                                                                                                                gCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGAcc                                                                                     >  1:186749/1‑251 (MQ=255)                                                                                                                                 aaCTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGAGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCt                                                                    >  1:246045/1‑251 (MQ=255)                                                                                                                                              tCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTg                                                       >  1:61282/1‑251 (MQ=255)                                                                                                                                               ccTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTg                                                       <  1:199530/250‑1 (MQ=255)                                                                                                                                                    tGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCAtt                                                 >  1:105927/1‑251 (MQ=255)                                                                                                                                                                gcccGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGAGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTgg                                     >  2:62092/2‑251 (MQ=255)                                                                                                                                                                     gTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCa                                 >  1:201535/1‑250 (MQ=255)                                                                                                                                                                                        tGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTGCTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcggc             >  1:27430/1‑251 (MQ=255)                                                                                                                                                                                          aCGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcggcgg           <  2:191457/251‑1 (MQ=255)                                                                                                                                                                                                   aTGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGgcg  >  1:73742/1‑251 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGGCG  >  NC_000913/1112223‑1112667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑