Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,110,033	G→A	Q233Q (CAG→CAA)	opgG →	osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,110,033	0	G	A	100.0%	71.1 / NA	24	Q233Q (CAG→CAA)	opgG	osmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

GGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGT  >  NC_000913/1109791‑1110271                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               ggggCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAat                                                                                                                                                                                                                                        >  2:110783/1‑251 (MQ=255)  gggCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAatc                                                                                                                                                                                                                                       <  1:78662/251‑1 (MQ=255)        gCTATTTCCGCGTCAATCGAGCAGGTCAGAATTATGGCCATACTGCCCGCGGCATGGAAATTGATACAGACTTGCAATCGGACGAAGAATTGCCGCGCTTCCAATAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGAGCGACAGGTACTTACAAATTCGTGGTTATGCCAGGTCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTg                                                                                                                                                                                                                                 >  2:36484/1‑251 (MQ=255)               ccGCGTGATTGGTGCAGGGCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGAt                                                                                                                                                                                                                           <  1:253261/250‑1 (MQ=255)                 gcgTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATaa                                                                                                                                                                                                                         >  1:266392/1‑250 (MQ=255)                     gATTGTTGCAGGGCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACGCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTGGTGGATGTGCAATCGAAAATCTATCTGCGCTATAAAGTCg                                                                                                                                                                                                                    <  2:224712/251‑1 (MQ=255)                                 tcaggtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTggggg                                                                                                                                                                                                        >  1:55085/1‑251 (MQ=255)                                                ttCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAAcc                                                                                                                                                                                          <  2:213386/250‑1 (MQ=255)                                                       cgcgGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGtt                                                                                                                                                                                  <  1:203337/251‑1 (MQ=255)                                                                                                      gcgcTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAAGAACTATCGTCCgg                                                                                                                                   <  1:83950/251‑1 (MQ=255)                                                                                                                      tGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTa                                                                                                                    >  1:211045/1‑250 (MQ=255)                                                                                                                             aGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGtctc                                                                                                            >  2:89757/1‑251 (MQ=255)                                                                                                                                    aaaaCCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATcc                                                                                                     >  1:110362/1‑251 (MQ=255)                                                                                                                                     aaccctaCTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATcc                                                                                                     >  2:64951/7‑250 (MQ=255)                                                                                                                                                  aaCGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGc                                                                                       <  1:110783/251‑1 (MQ=255)                                                                                                                                                     gTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGa                                                                                     >  2:74305/1‑250 (MQ=255)                                                                                                                                                            cATCTATGCATTGCTTGACTCGCCGAGTGCGACAGGAGCTTACACATTCGTGGATATGCAAGGGCGTGTCACGGTTGTGGCTGTGCAATAGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTATCTCTATCCCTGCCGGTAATGGCGAATGGATCt                                                                             >  2:265663/1‑251 (MQ=255)                                                                                                                                                                tATGCCTTGTTTGTCTCGCTGCGTGCGACAGGTGCTTCCAAATTCGGTGTCATGCCAGGGCGTGACTCGGTTGTGGAGGTGCAATTGAAAATCAAGCTCCGCGATAAAGTCGGCAAACTGGGGGTTTTACTGTTCACCAGTACGCTGTTGGTGGAGCCGAACCAACCGTGGCCTGCAAATAACTATCGTCCGGAGTTGCATGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGc                                                                          <  1:259306/250‑1 (MQ=255)                                                                                                                                                                            gACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATa                                                              >  1:158031/1‑250 (MQ=255)                                                                                                                                                                                            cAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCg                                             <  1:148017/251‑1 (MQ=255)                                                                                                                                                                                                    tACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTGCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCGCTCTATCCATGCCGGTAATGGCGAATGGATCGGGCGTCCGTTGAATAACCCGAAACATTGAGCTGTcagc                                       <  2:110362/249‑1 (MQ=255)                                                                                                                                                                                                          gtcgagggtATGTCAGGGCGTGACAGGGTTGTGGATGTGCAATCGAACATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTGTGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGATAGGATCTGGCGTCGCATGAATACTCCAAAACATTTAGTGGTCACCAGCTtct                                >  2:18445/9‑250 (MQ=255)                                                                                                                                                                                                                   aTGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGATTGGATCTGTCGTCCGGTGAATAACCCGAAACATTTAGCGGTCAGCAGCTGCTTCATGGaaa                       <  2:158031/250‑1 (MQ=255)                                                                                                                                                                                                                                       tgtgGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCCTGGAAAACCCGCAAGGCTTTGGTCTGt  >  1:159085/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               GGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTATCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGT  >  NC_000913/1109791‑1110271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑