Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,740	C→T	L136F (CTT→TTT)	opgG →	osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,740	0	C	T	100.0%	42.4 / NA	14	L136F (CTT→TTT)	opgG	osmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/6);  total (8/6)

CAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAA  >  NC_000913/1109512‑1109924                                                                                                                                                                                                                                     |                                                                                                                                                                                         cagcagATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCaa                                                                                                                                                                       >  2:78662/1‑248 (MQ=255)               tAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTGCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGGAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGaa                                                                                                                                                       <  1:88604/250‑1 (MQ=255)                            gCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCt                                                                                                                                         >  1:12134/1‑251 (MQ=255)                                                               cGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACGGCCACCGCAGTCAAACAAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTTCCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGcaggtc                                                                                                      <  1:127406/251‑1 (MQ=255)                                                                    tCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGGGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGcaggtcaggt                                                                                                  <  2:65323/250‑1 (MQ=255)                                                                       aCCATCAGGGTATGTACTTCGATACCCCGGTCAAAATCAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTAt                                                                                              <  2:78170/251‑1 (MQ=255)                                                                          aTCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGc                                                                                           <  1:42602/251‑1 (MQ=255)                                                                                               ccccGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAACCGAATCAAATACAGCCAGGATTATTTCACTTACGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGc                                                                       >  2:253261/1‑250 (MQ=255)                                                                                               ccccGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCACGCGGCCTGGc                                                                       >  1:213386/1‑250 (MQ=255)                                                                                                                                        cGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCCGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCtt                             >  2:148017/1‑251 (MQ=255)                                                                                                                                                 tACAGCCCGGATTATTTCACTTTCGGCGATGCTCAGCATGACAACGATACGGTAAAAGAACTTGGATTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGAATGCTAGAGGCCAGATATTTCCGCGAGATTGGAGCAGGACAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTACAATCGGGTGAAGAATTTCCGCGCTTAAAAGAGtt                    >  1:23814/1‑251 (MQ=255)                                                                                                                                                  aCAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGAGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCACTTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTc                   >  2:83950/1‑251 (MQ=255)                                                                                                                                                   cAGCCCGGCTTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGCTCAACAGCAAAGATAAAAACGATGACATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCt                  >  1:224712/1‑251 (MQ=255)                                                                                                                                                                   ccttgcgtcGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTGGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATTCCGCCTTTCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCTAGCGTCCaaa  <  2:12134/243‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                         CAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAA  >  NC_000913/1109512‑1109924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑