Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,090,508	C→T	E594E (GAG→GAA)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,090,508	0	C	T	100.0%	33.7 / NA	12	E594E (GAG→GAA)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/4);  total (8/4)

CAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCT  >  NC_000913/1090285‑1090736                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     cAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGa                                                                                                                                                                                                            <  2:95801/250‑1 (MQ=255)                            tACGCCGCTCATTTTAATCCCAGCGAAAATAAGCCTGAGCACTGTTGCCTGTAACACTATTTTTCATTGCCCGTAATGGACCGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCAAGACAGGCGCGCGCGGGGTTTATGCTCATGAGTGAAAACGCGTTCAGCGTATGCTGCCGCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTc                                                                                                                                                                                 <  1:172766/250‑1 (MQ=255)                                                     aaCATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTa                                                                                                                                                        >  1:61069/1‑250 (MQ=255)                                                                               tAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATcc                                                                                                                              >  1:111062/1‑250 (MQ=255)                                                                                               tGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGt                                                                                                             >  2:59699/1‑251 (MQ=255)                                                                                                                               ccAGTTGCGAACCAATCCCCCATTTATCATAAAAATCATACCACCCAGACAGGCGCGCGCGGGGTTTATGCCAATGTTGGAAACCGCGTTCAGCTTATTCTGCCCCGAGCCAAATATGACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGAtggtg                                                                             <  1:59699/251‑1 (MQ=255)                                                                                                                                 aGTTGCAAACCAAACCGCCGGTTATCATAAAATTCATCCCAGCCAGACGGCCCCGCCCCGGGTTTAGGCTCATGATTAAAAACGCGTCCAGCGTATTCTCCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCTGTGGTGAATAAACGATggtggt                                                                           <  1:202537/251‑1 (MQ=255)                                                                                                                                                      ttATCATTAAAATCATCCCCGCCGGACAGCCGCGCGCCGGGTTTATGCTCATGATTGCAAACGAGTTCAGCGTATTCTGCCTCGAGCCCGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCACAACCGCGCCAGTTATCCTTCAGCGGGGGTGAATAAACGATGGTGGTTACGTAGACATCATGTTTAcc                                                      >  2:265742/1‑251 (MQ=255)                                                                                                                                                               aaaTCATACCAGCCAGACCGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCAAGTTATCCTTCATCGGTGGGGAATAAACGATGGTGGTTACGTCGACATCATGTTTACCACTATCCgg                                             >  2:169706/1‑251 (MQ=255)                                                                                                                                                                   cATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGAACGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATACCCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCt                                         >  1:154399/1‑251 (MQ=255)                                                                                                                                                                                                     cTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAACTTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGa        >  1:7704/1‑250 (MQ=255)                                                                                                                                                                                                          gATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGctct  >  1:50799/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     CAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCT  >  NC_000913/1090285‑1090736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑