| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,086,153 | C→T | G123R (GGG→AGG) | pgaD ← | poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,086,153 | 0 | C | T | 100.0% | 39.2 / NA | 15 | G123R (GGG→AGG) | pgaD | poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/8); total (7/8) | |||||||||||
GATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGT > NC_000913/1085913‑1086396 | caTTTGCCTCGCGTCTTGTGCTCCGTATTAAGTGACGCGCTGGATCGTTTCGAAGAAGATGAAATGGTTGGTATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACTTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGTGCTTTGTTTTGGGTTTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTg < 2:211529/250‑1 (MQ=255) tCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTgg > 1:116660/1‑251 (MQ=255) ccGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCTGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTc < 1:45614/251‑1 (MQ=255) gTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTgcggcg > 1:247172/1‑251 (MQ=255) tGCCAACGTACGCTGCATGCCTACAGGTAAGTGTGTTAGCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTTATCCTGTGGCTTTTTTGTAGTTGCTTATAGAGCTCATCAGGTAGTGCTAAGCTCTCTGCATATTCTTGCGGCGTAAATTGGTAGGCTGCATGATGctgctg < 2:242169/250‑1 (MQ=255) gCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTGTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTa < 1:46414/251‑1 (MQ=255) aGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTTTTGTAc < 2:254134/251‑1 (MQ=255) tAAGTGTGTTATCGGTGCAGAGCACGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAgc > 1:142002/1‑251 (MQ=255) aaGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGc > 1:79923/1‑238 (MQ=255) aaGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGc < 2:79923/238‑1 (MQ=255) ccGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGc > 1:25764/1‑251 (MQ=255) gcTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCATCAAAAAATAGAACTGAAGTCGGCTTCTGGc < 1:47176/251‑1 (MQ=255) cTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGcc > 2:265060/1‑251 (MQ=255) aaaCAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGc > 2:215628/1‑251 (MQ=255) aTTTGCCTCTGTCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGGAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATGCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCTCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGt < 1:261741/251‑1 (MQ=255) | GATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGT > NC_000913/1085913‑1086396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |