Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,078,268	A→T	F205L (TTT→TTA)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,078,268	0	A	T	100.0%	50.4 / NA	18	F205L (TTT→TTA)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/8);  total (10/8)

AAGAGTAACGGAAACCTTTCTCTTCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGC  >  NC_000913/1078033‑1078513                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      aaGAGTAACGGAAACCTTTCTCTTCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCt                                                                                                                                                                                                                                         <  1:142630/250‑1 (MQ=255)                                      aTTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGAttt                                                                                                                                                                                                   >  1:221949/1‑251 (MQ=255)                                          gCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTc                                                                                                                                                                                               <  1:227087/251‑1 (MQ=255)                                                     cgcgATGGTTTCGCCAGTTACGAACTGCTCCCTCATCTGGCGCATCGCCATATCAACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATACTGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGGAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCCTTCACTAACAGTGACGGGCTACAACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAAcg                                                                                                                                                                                    >  2:50202/1‑251 (MQ=255)                                                                     gTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCCGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCttt                                                                                                                                                                    >  2:262352/1‑251 (MQ=255)                                                                                                    ccATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTGTGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAgc                                                                                                                                     >  1:153166/1‑250 (MQ=255)                                                                                                                       gATCAGCGGTTCAACGCTTATACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTCTGGGTGGACACCAGTTTGCCCGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTCACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCCCGCCtt                                                                                                                  >  2:161904/1‑251 (MQ=255)                                                                                                                             cGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGACTACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCg                                                                                                            >  1:116609/1‑251 (MQ=255)                                                                                                                                      gCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGGGGCGGCATTAACTAACAGTGACGGGCTACGACCACTGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTTTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCg                                                                                                   >  1:184208/1‑251 (MQ=255)                                                                                                                                                                 gcgGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTTGACCa                                                                        <  2:221949/251‑1 (MQ=255)                                                                                                                                                                      agagGCTGGCGTCGTTATGGGTGGAAACCAGTGTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACGGCGAAAACTCCCGCAATAACCCCTGGACCATACCt                                                                   <  1:72008/251‑1 (MQ=255)                                                                                                                                                                              gctTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGAcc                                                           <  1:26771/251‑1 (MQ=255)                                                                                                                                                                                          gTGGAAACCAGTTTGCCAGTAAACAGCATCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGTAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACTCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCAttttt                                               <  1:161904/251‑1 (MQ=255)                                                                                                                                                                                          gTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCAttttt                                               <  2:85774/251‑1 (MQ=255)                                                                                                                                                                                                             tAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCg                            >  1:133774/1‑251 (MQ=255)                                                                                                                                                                                                              aaaCAGCAGCCCCCAGGTCGCGGCATTAACTAACCGTGACGGGCTACGACCAATGTGTGCCTGCCAGTTACCGTTGCTGATTTTGTCGCGACTTAACGCGTCGCGGGTGGCTTTGTCGGGAATCCGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGCCCACTGGCCTTTTTTTGATTACGCAGATTATCgg                           >  2:102483/1‑251 (MQ=255)                                                                                                                                                                                                                 cagcagCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCa                        <  1:184778/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTgcgc  >  1:126344/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      AAGAGTAACGGAAACCTTTCTCTTCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGC  >  NC_000913/1078033‑1078513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑