| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,075,234 | C→T | V1217I (GTA→ATA) | putA ← | fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,075,234 | 0 | C | T | 94.3% | 88.5 / ‑5.7 | 35 | V1217I (GTA→ATA) | putA | fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (18/15); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
ATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTACCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAA > NC_000913/1074987‑1075480 | atatACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCTAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGCTGCAGCGCGTCATCCGGCCACAGTATCTg > 1:269428/1‑251 (MQ=255) aaaGGATATTGCTTTCGCCCCGGGAAAATCCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCACCTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGc < 1:36294/250‑1 (MQ=255) cggATATTGATTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGGGCCTTCCCGCGCGGCAACTGCTTCACCCAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTACCTGGCTGCCCACCGCCAGCAc > 2:40528/2‑251 (MQ=255) gATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGGTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCGGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCAcgg > 2:56813/1‑251 (MQ=255) aaaaCCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGTAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCAt > 1:151131/1‑251 (MQ=255) aTCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACacaaca < 1:119992/251‑1 (MQ=255) gcgGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTc > 1:102244/1‑251 (MQ=255) ggCAACTGCTTCACACAAGGGGCGAGGCGGATCCGAATCACCGTGGAAGATCCCCGCACAAAACGGTTGGGCGGGAATATTTTCCGCTTTCGCCATTTGAATACTGTCGTTGACTGCCGATGG‑CAATGCCTTCACAAACTGACGATGCACCGCGCCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGTCGGCGAGCTGATTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCTCTCAc < 2:261268/251‑1 (MQ=255) gcaccTGCTTCACACAATGCGCGAAGCGGATCCGAATCACCGGGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACGAACGGACGATGCAGCGCGTCACCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAcg < 2:102244/247‑1 (MQ=255) gCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGgc > 1:77691/1‑249 (MQ=255) acaAGGCGGGAAGCTGAGCCGAATCCCCGTGGAAGATCCCCGCATCAACCGTTGGAGCGGTTATATTTTCCGCTTTCGCAAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTc < 2:197910/251‑1 (MQ=255) aaTGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTcc > 1:182122/1‑250 (MQ=255) gATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTc > 1:47325/1‑250 (MQ=255) ccGTGGAAGACCACCCCAACAAACGGTTGAGGGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACGGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGccc < 2:1686/251‑1 (MQ=255) gTGGAAGTTCCCCGCATCAACCGGTTGGGCGTTTATATTTGCCGCTTTCGCCGGTTGAATACGTTCGTTGACTGCCGATGG‑CAAGGCCTTCACTACCTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCg < 2:6551/251‑1 (MQ=255) gTGGAAGATCACCGCATCACTCGGTTGAGCGGTTATATTTTCCGATTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATATGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGAGCTGCGTTAACACGTCGGCCCCg > 2:265781/1‑251 (MQ=255) gTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCg > 1:172714/1‑251 (MQ=255) cGCATCAAACTGTGGAGCGGTGATATTTTCCCCTTTCGCCAGTTGAACACGTTCGCTGACTGCCG‑TGGTCAAGGCCTGCACCAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCGCGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAAGACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGGGTTGCGTTCACCCGTCGGCCCCGGCAGCATTCGtt < 2:116149/251‑1 (MQ=255) ggTTGAGCGGTTATAGTTTCCGCTGTCGCTGGTTAAATCCGTTCGCTGACTGCTGATGG‑CAATTCCTTCACTAACGGCCGATGCAGCGTGTCTTCCGGCCACATTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCTGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGGGTTcctg < 2:145510/250‑1 (MQ=255) gCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGATGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTgcgc > 1:252877/1‑251 (MQ=255) ttCCGCTTTCGCCAGTTGAATCCTTGCTCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCCCAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCa < 1:56813/250‑1 (MQ=255) gccgctatcggaatccgctccgtGACTGCCTATGG‑CAATGCCTTCCTTAACCGACGAGGCTGCGCGCCTCCCGGCCACAGTTTCTGGCTCCCCACCGCCCGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCAGCGTCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTGGCGTGCACCCGTCGGCCCCGTCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGc < 2:269428/227‑1 (MQ=255) aGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAc > 2:90300/1‑157 (MQ=255) aGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCAc < 1:90300/157‑1 (MQ=255) aTACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTc > 1:172298/1‑186 (MQ=255) gACTGCCGATGG‑TAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCGGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGAc < 2:62661/251‑1 (MQ=255) cTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACg > 1:58499/1‑250 (MQ=255) gCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTc > 1:116317/1‑251 (MQ=255) aaCTGACGATGCAGCGCGCCATCCGGCCACCGTATCTGGCTGCCCACCGCCAGAACGGCGGCGAGCTGAGTCAGCGAATCCTGCTCATACTCGGCAATACACAACACGCGCTAACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCAGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCCTATTGCGTACCTAACGCCTGCAATTATGGACGATTTACTGCCCATTCCCACAGTGc > 2:192392/1‑251 (MQ=255) aCTGACGATGCAGCGCGTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGc > 1:189600/1‑250 (MQ=255) taCGATGCAGCGCTTCACCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTTCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCAtt < 1:226255/249‑1 (MQ=255) cAGCGCTTCATCCGGCCACAGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGGCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCTTCCAGGTGTTTCGTTCACCCGTCGGCCCCGGCATCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCt < 1:268449/251‑1 (MQ=255) cATCCAGCCCCAGTATCTGGCTGCCAACCGCCAGCACGCCGGCGAGCTGAATCAGCGCATCCTGCTCATCATCGGCAATACACCACACGCGCTCACGCGGCCGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCCTTTAGCGGCTGAGTAAAt > 2:239195/1‑251 (MQ=255) aGTATCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCa > 2:183797/1‑250 (MQ=255) aGTACCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATTCACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCaa < 1:183946/251‑1 (MQ=255) | ATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCATCCCGCGCGGCAACTGCTTCACACAATGCGCGAAGCTGATCCGAATCACCGTGGAAGATCACCGCATCAAACGGTTGAGCGGTTATATTTTCCGCTTTCGCCAGTTGAATACGTTCGCTGACTGCCGATGG‑CAATGCCTTCACTAACTGACGATGCAGCGCGTCATCCGGCCACAGTACCTGGCTGCCCACCGCCAGCACGGCGGCGAGCTGAGTCAGCGCATCCTGCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAA > NC_000913/1074987‑1075480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |