Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,699	C→T	A372V (GCC→GTC)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,699	0	C	T	100.0%	54.2 / NA	19	A372V (GCC→GTC)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/13);  total (6/13)

ATCGACAAAGCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAA  >  NC_000913/1066455‑1066941                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   atCGACAAAGCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCTGAACAGTTACGTAATGTCGATg                                                                                                                                                                                                                                               <  1:71710/250‑1 (MQ=255)                      gATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATTGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCTCCTgc                                                                                                                                                                                                                         <  2:32797/251‑1 (MQ=255)                            aCCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCg                                                                                                                                                                                                                   >  1:3608/1‑251 (MQ=255)                             ccAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCgt                                                                                                                                                                                                                  >  2:172115/1‑251 (MQ=255)                                     ccGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGTACCTGCGCAGCGTGTGACGCt                                                                                                                                                                                                          <  1:8188/251‑1 (MQ=255)                                                          gggCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGAt                                                                                                                                                                                     <  2:83720/251‑1 (MQ=255)                                                                    ccAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGACAATCGCGATGTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGAt                                                                                                                                                                           <  1:175176/251‑1 (MQ=255)                                                                                  ctGTTAACGGCGCTGGATTTCACACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGTATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGCCGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGAt                                                                                                                                                             >  1:38262/1‑251 (MQ=255)                                                                                   tGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                                                                                                                                            >  1:247518/1‑251 (MQ=255)                                                                                                                                           ccGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCcgcg                                                                                                    <  1:245981/251‑1 (MQ=255)                                                                                                                                             gATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGTAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCcgcg                                                                                                    >  2:181825/1‑249 (MQ=255)                                                                                                                                                          aTCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTc                                                                                     <  2:3608/251‑1 (MQ=255)                                                                                                                                                                 tCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAGCAGAGTGCGGAACAGTTACGTAATGTCGATGCGGTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGAGAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGt                                                                               <  1:136932/250‑1 (MQ=255)                                                                                                                                                                                                               tGAATATGTGTATCAGAGTGCGTAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAAt                                 <  1:181825/250‑1 (MQ=255)                                                                                                                                                                                                                gAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATc                                <  1:170657/250‑1 (MQ=255)                                                                                                                                                                                                                      gtgtATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCTGGGGAGTCGCTGGTTAAACGTTGTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATc                          <  1:19745/250‑1 (MQ=255)                                                                                                                                                                                                                      gtgtATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATc                          <  1:191671/250‑1 (MQ=255)                                                                                                                                                                                                                        gtATCAGAGTGCGGAACAGGTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCTGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAATCGAATCTTTATCGGt                       <  2:38262/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGCTGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTac  >  2:180644/1‑249 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   ATCGACAAAGCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAA  >  NC_000913/1066455‑1066941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑