Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	908,169	C→T	R38R (AGG→AGA)	ybjT ←	putative NAD(P)‑binding protein YbjT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	908,169	0	C	T	97.5%	125.1 / NA	40	R38R (AGG→AGA)	ybjT	putative NAD(P)‑binding protein YbjT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/13);  minor base G (0/1);  total (26/14)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGCCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTT  >  NC_000913/907924‑908412                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gACTGCTCATGTGGGGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTc                                                                                                                                                                                                                                                <  2:12640/251‑1 (MQ=255)                           cGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCt                                                                                                                                                                                                                      <  1:82813/251‑1 (MQ=255)                                       gaTTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTgct                                                                                                                                                                                                          >  2:73374/1‑251 (MQ=255)                                                cTTAAATGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAgtg                                                                                                                                                                                                 >  2:163777/1‑251 (MQ=255)                                                 ttcaCTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTT‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGACAGGCTCTGCTGCCCTTGCTGTCTGAgtgt                                                                                                                                                                                                >  2:151381/4‑251 (MQ=255)                                                             tCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATg                                                                                                                                                                                    >  2:124427/1‑251 (MQ=255)                                                               ccGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATTCCCTTGCTGGCTGAGTGTGCGCACCAGATGCt                                                                                                                                                                                  <  1:231643/250‑1 (MQ=255)                                                                   aGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCCGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGAcc                                                                                                                                                                              <  2:102495/251‑1 (MQ=255)                                                                          cgcgGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGt                                                                                                                                                                         >  1:108225/1‑249 (MQ=255)                                                                                            ccTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACt                                                                                                                                                     >  1:67499/1‑251 (MQ=255)                                                                                              tGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTa                                                                                                                                                    >  1:96391/1‑250 (MQ=255)                                                                                               ggCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAACTAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGCACTaa                                                                                                                                                   >  2:204936/1‑250 (MQ=255)                                                                                               ggCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCAATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTaa                                                                                                                                                   >  2:48227/1‑250 (MQ=255)                                                                                               ggCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCAGGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTa                                                                                                                                                    <  2:229958/249‑1 (MQ=255)                                                                                                            gATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCAAGCTGAGATCGACATTATGGCAACTGAAGTTTGACAGATG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACAGAGAACTAAAATGCGTTGCGGc                                                                                                                                      >  1:260327/1‑250 (MQ=255)                                                                                                                     gccgccTTCGCCCATGCTGTGCACCAGAAACTAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGCGAACTCAAATGCATTGAGGCACGtctctct                                                                                                                            >  2:45487/1‑251 (MQ=255)                                                                                                                        gccTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCACTGTAGCCACTGGCACCGAGAACTAAACTGCGTTGCGGCACGTCTCTCTCCt                                                                                                                         >  2:41697/1‑251 (MQ=255)                                                                                                                        gccTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCt                                                                                                                         <  1:73374/251‑1 (MQ=255)                                                                                                                           ttCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTa                                                                                                                       >  2:196508/1‑250 (MQ=255)                                                                                                                                             cAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGc                                                                                                    >  1:195434/1‑251 (MQ=255)                                                                                                                                                   aTAGACAGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCAGGCCAGCTGAGATCGACTTTATGGAAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGAGGCACGTCTCTCTCCTTAACGCGCCAGGACTGCACGCCAgggg                                                                                              >  2:37226/1‑248 (MQ=255)                                                                                                                                                    caGACCGTATCGATACCCGTCAACAGGTCCGGGAGGTTATCCGTCCAGCTGAGATCGCCGTTAGGGCAACTGACGTTTGCCAGTTG‑CAGGTTGGCAAGTCTGGCGACAGGACTTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTCCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAATTGCGTTGCGGCACGTCTCTCCCCTTAACGCGCCAGTAATGCTCGCCAGTggg                                                                                             <  1:233336/250‑1 (MQ=255)                                                                                                                                                          gaatcgatctccgtcAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCGGTCGACATGACGTGCTGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCGCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGa                                                                                       <  2:1606/237‑1 (MQ=255)                                                                                                                                                            aTCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACt                                                                                     >  1:242319/1‑251 (MQ=255)                                                                                                                                                                  ttCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTACGGCAACTGACGATTGACAGTTG‑CAGCTTTGAACGTCTGTCGACATGCCGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGTGGACCAATGTAGCCACTGGCACCGAGCACTCAAATGCGTTGCGGCACGCCTCTCTCCTTAACTCGCCAGGAATGCACGCCCGTGGGCGGCGACTTcctcc                                                                               >  2:9836/2‑248 (MQ=255)                                                                                                                                                                             gggCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTcgcg                                                                    >  1:206713/1‑251 (MQ=255)                                                                                                                                                                                                  aGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCAc                                               >  1:85942/1‑251 (MQ=255)                                                                                                                                                                                                         aacGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACAGGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCgg                                        <  2:216920/249‑1 (MQ=255)                                                                                                                                                                                                              cTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAAt                                   >  1:95561/1‑251 (MQ=255)                                                                                                                                                                                                                gtATGGCAACTGACGTGTGCC‑GTTGGCAGCTTTGCTAGTCGGGCGACATGACGCGCCGTCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGGGCGCCCCAGATGCTGACCAATGTAGCCACTGGCACCGAGAGCTAAAACGCGGTGCGGCCCTTGGCTCGGCTTAACGCTCCAGGAATGCGCGCCAGGGGGCGGCGACTGCCGCCAGTTGTGCGTGCGAGACGGCAAGATGCGTGGCCAGGCGGGCAATc                                  <  2:112062/249‑1 (MQ=255)                                                                                                                                                                                                                      cAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACAGGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAgg                           <  1:82516/251‑1 (MQ=255)                                                                                                                                                                                                                        aCTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCAGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCg                         >  1:165207/1‑251 (MQ=255)                                                                                                                                                                                                                         cTGACGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGt                        >  2:174479/1‑251 (MQ=255)                                                                                                                                                                                                                            aCGTTTGCCAGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTa                      >  1:74866/1‑250 (MQ=255)                                                                                                                                                                                                                              gTTTGCCAGTTG‑CAGCTTTGCAAGTCTGACGACATGACGTGCCGCCGACAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGAGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCCAGGTGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATc                   >  2:231110/1‑251 (MQ=255)                                                                                                                                                                                                                                     aGTTG‑CAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGt            >  2:217275/1‑251 (MQ=255)                                                                                                                                                                                                                                                ttGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGggcggc                                                                                         <  1:141447/164‑1 (MQ=255)                                                                                                                                                                                                                                                ttGCAAGTCTGTAGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGggcggc                                                                                         >  2:141447/1‑164 (MQ=255)                                                                                                                                                                                                                                                ttGCAAGGCTGTCGACATGCCGTCCCGCCGCCAGGATCTGTTGCCCTTGCGTGCTGGGTGAGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCt    <  2:2947/249‑1 (MQ=255)                                                                                                                                                                                                                                                 tGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCttt  <  1:163777/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GACTGCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTG‑CAGCTTTGCAAGCCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTT  >  NC_000913/907924‑908412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑