Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,527	C→T	E405K (GAA→AAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,527	0	C	T	100.0%	50.7 / NA	19	E405K (GAA→AAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/8);  total (11/8)

CCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGAT  >  NC_000913/822302‑822773                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        ccGTAACTCAGATCGCTGACGAAAACCAGCCCGACCGCACTAAGCAGAGCAACGCGATTGTAGTCGAGGCTGACCTTCCAGCCCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATCAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGAGGGCGTGCTTTACTCATCAGGGTCTTTCCCACGTTTTTTCAGTTTTTACCCGATCCTGTGTTTTCTTTGTc                                                                                                                                                                                                                                >  2:114456/1‑251 (MQ=255)   gTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCgg                                                                                                                                                                                                                              >  1:28838/1‑251 (MQ=255)         cAGATAGCTGCCGACCCCCCGCCCGCCCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCTATCCGGCGTTTCCATTGTCGGGTGTgc                                                                                                                                                                                                                        <  2:177241/251‑1 (MQ=255)         cAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTTCCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTgc                                                                                                                                                                                                                        <  2:19758/251‑1 (MQ=255)                                       aCTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCAACGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTACGCGCCAGCGCGGGCGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGGTCAACCTgaccccgacggggcgt                                                                                                                                                                                          >  1:155931/1‑236 (MQ=255)                                                                 cgcgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAACCAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGGGTGCCGGAAGCCAG‑CCAACCAgcg                                                                                                                                                               >  2:115709/1‑251 (MQ=255)                                                                       tGACCTTCCCGACATGTTCACAGACAACTTTTTTGGCGTAGACCACCAGCCACACAATCACCGCGATAAAAAAAAGCCAGGTGAGGATCTTCTTTGCTACGCGCCACAGCGGGAGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCAGCGTTTACATCGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTCACGTGTGTGCCGGAAGCCAGGCCAAcacggccggc                                                                                                                                                           >  2:245155/1‑241 (MQ=255)                                                                           cTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCCACACAATCACCGCGATAAAAATCAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGAAGTGGCGTGTGGGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTg                                                                                                                                                     >  2:30394/1‑251 (MQ=255)                                                                                aGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAAACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGt                                                                                                                                                 >  2:136119/1‑250 (MQ=255)                                                                                             gTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGGAAAAAGTGGAACGc                                                                                                                                   >  1:188210/1‑251 (MQ=255)                                                                                                     ttttGGCGTAGACCACCAGCCACACAATCACCGCGATAAACAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGCTCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCACCCTGCGCCAGACTTGGCGTGGGTGCCGGAAGCCAG‑CCAACCAGCCCCGGGAAGTGGCGTAAAAAATGGAACGCCAGCACGc                                                                                                                           >  2:166706/1‑251 (MQ=255)                                                                                                                                                                     tGCTAAGCGCCAGCCTGGGTGTGATTAACTCTTCATGGTTTTATCCCCTTGGTTTCAGTTTTTACACGATCCCACGTTTCCATTGTCGGTTGTGCGGGCGGAGCAACCTGCGCCAGACGTGGCGTGTGGGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAGCGCCAGCACGTGTTTGGTCAGGTTCCACCAGGTGCTTTTGGGCAGCATTGTGTCATCCACCTACTGACATTCTg                                                            <  2:245891/250‑1 (MQ=255)                                                                                                                                                                                     gggTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTc                                           <  2:28838/251‑1 (MQ=255)                                                                                                                                                                                     gggTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAAACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTc                                           <  1:136119/251‑1 (MQ=255)                                                                                                                                                                                                       aGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTc                         >  1:189045/1‑251 (MQ=255)                                                                                                                                                                                                            tttACCCCCGTGTTTTCAGTTTTTACCTGCTCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATAAACCTGCGCCAGACGTGGCGTGTGGGCCGGAAGCCAG‑CCAACCCACGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGATCCACCAGGTGCGGTAGGGCAGCATGGTTTCATCCACCTGCTGACAAACTGGCGCAATAATGCCGTTCAGATTTTCTCGCAGCGTCTGGtt                    >  2:18612/1‑251 (MQ=255)                                                                                                                                                                                                             tttcccccTTGTTTTCAGTTTTTACCCGACCCTGCGTTTCCATTGTCGGGTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGGTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCTCATCGTCTGGtt                    <  2:189045/247‑1 (MQ=255)                                                                                                                                                                                                                            cAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCAGCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTgg    <  1:29419/251‑1 (MQ=255)                                                                                                                                                                                                                              gTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGgat  <  1:75679/251‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        CCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAG‑CCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGAT  >  NC_000913/822302‑822773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑