Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,508	A→G	Q724Q (CAA→CAG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,508	0	A	G	100.0%	40.9 / NA	17	Q724Q (CAA→CAG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/11);  total (6/11)

ATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTG  >  NC_000913/2261‑2746                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               atatatTCGGCAAGTTAGACTAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTAGACCGAACCGGATCCGCGAGATGATCTTGCTGGTATGGATGTAGCGCGGAAGCTATTGATTCGCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCTCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTc                                                                                                                                                                                                                                             <  2:186638/251‑1 (MQ=255)                                 ttCTCCGTGGCGACTACGCCGGTGCGGGAAATGGGTTATACCGAACAGGATCCGCGAGATGATCTTTCTGGTAGGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCTTGTCGAAgg                                                                                                                                                                                                              <  2:217794/250‑1 (MQ=255)                                     ccGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGTGACGTGAACTTGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGAAGATCACTTTGCCGCGCCCGTGTCGAAGGcc                                                                                                                                                                                                            <  2:161953/248‑1 (MQ=255)                                                              aaTGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTTCGCTATg                                                                                                                                                                                <  2:245909/251‑1 (MQ=255)                                                                      ataCCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGGTTTGCGCTATGTTGGCAat                                                                                                                                                                        <  1:193031/251‑1 (MQ=255)                                                                                       cgAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTTGCAATATTGATGAAGATggtgc                                                                                                                                                       <  1:212769/251‑4 (MQ=255)                                                                                        gagaTGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATggtgcc                                                                                                                                                      <  2:182701/251‑5 (MQ=255)                                                                                                cTTTCTGGTATGGATGTAGCGCGTAAGCTATTGCTTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGCAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGGGCCTGCCGCGt                                                                                                                                              >  2:263224/1‑251 (MQ=255)                                                                                                        tATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGGGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGACACAGCTCGACGATCGCTTTGCAGCGAGCGAGGCGAAGGCCCGTGATGAAGGAAAAGTTTGGAGCTATGTGGGCAAGATTGAGGAAGATGGAGCATGCCGCGTGAAGaatg                                                                                                                                      >  1:149474/1‑248 (MQ=255)                                                                                                                                      cgctcgTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTc                                                                                                         <  1:264836/250‑1 (MQ=255)                                                                                                                                                                aGCTGGCGGATATTGAGATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATAGTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTg                                                                              <  2:26864/251‑1 (MQ=255)                                                                                                                                                                                        cTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGAGGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATc                                                        >  1:249246/1‑249 (MQ=255)                                                                                                                                                                                                              cgctgAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGGTCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGGGGATGGTAATGATCAGCTGTTCAAAGTGAAAAATGGCGAAAACGACCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGcgcg                                >  2:82174/5‑251 (MQ=255)                                                                                                                                                                                                                cggAGGGTGATGTTGCCGCTTTTATGGGGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAAGGGCGAAAACGCCCTGGCCTTTTAGAGCCACTATTATCAGCCGCGGCCGTTGGTGCTGCGCgg                               <  2:215709/248‑1 (MQ=255)                                                                                                                                                                                                                  gAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCTAAGTGGATGGTAATGa                                                                                                                   <  1:36210/164‑1 (MQ=255)                                                                                                                                                                                                                  gAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTACTGa                                                                                                                   >  2:36210/1‑164 (MQ=255)                                                                                                                                                                                                                                            gAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAACCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTg  >  1:3795/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTG  >  NC_000913/2261‑2746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑