Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	43.5 / NA	16	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/7);  total (9/7)

GCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTA  >  NC_000913/696229‑696704                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           gCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCgg                                                                                                                                                                                                                                   >  1:106177/1‑251 (MQ=255)                cGTCAGGAGCTGACATATCCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACAGTAGGTCGGATAAGACGCCGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCGTTCGACATTGAATGAACGCGGAACAGCAAAAAGCTTGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAAAAACCGGGGCCTTACCgatggg                                                                                                                                                                                                                    >  2:114512/1‑246 (MQ=255)                             aaTATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACt                                                                                                                                                                                                       >  1:12140/1‑251 (MQ=255)                                   ggtgaGGGTTGTAGCCGTAAAACATGGCCGGATTCGTGCCAAACGTAGGCAGGATAAGACGCGGAAGAGTCGCAGACGACATTGAAGTATAAGACGTGTCAATATCGCATTCGACAATGAATGAACGCAGAAAAGCAAAAAGATCGCCGAAGCGAGCTTTTTTAAGGTGGTTGGGGTACGAGGATTAGAACCTCGGAATGCCGGAATTAGAATCCGGTGCTTTACCGCTTGGCGATACCCCAACTGGGTGc                                                                                                                                                                                                 <  1:207187/247‑1 (MQ=255)                                           ttGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGACCCTCGGACTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGCTGCACTTACa                                                                                                                                                                                          >  1:145779/1‑250 (MQ=255)                                                                                                              ccGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATc                                                                                                                      <  1:244376/251‑1 (MQ=255)                                                                                                                                         cATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGAAAGCGTATTGAATAAATTGGCAAGGGTGCGAGGATTCGAACCGCGGTAGGCCGGGATCACAATCGGGTGCCTTGCAGTTTGGCGATAaccc                                                                                           >  2:210469/1‑251 (MQ=25)                                                                                                                                          aTCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGCGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCCTTACAAGGTAAGCGTCTTGCATAAATTGGCTGGGGCACGAGGCTTAGAACGTCGGAATGCCGGCATCAGAAACGGGTGCCTTACCGCTTGCCGATAcccc                                                                                           >  1:18275/1‑250 (MQ=37)                                                                                                                                              cATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCTCGGAATGCCGGAATCAGAATCCAGTGCCTTACCGCGTGGAGATACCCcaaca                                                                                       >  1:223384/1‑250 (MQ=255)                                                                                                                                                        tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTCCCTTACCGCTTGGCGATACCCCAACAAATTGGtttt                                                                             <  1:103887/250‑1 (MQ=255)                                                                                                                                                                               agctcacggaatcgactttttttAATGTAGCTGGGGGACGAGGATTCGAGACTAGGAATGCCGTAATTAGAAGCCGGTGCCTTAGCGCTTGGCGATACCCCATCTGGGTGCACGTACAAGGGAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACATATTGGTTTTGAATTTGCCGAACATATTCGATAc                                                     <  1:10729/234‑1 (MQ=25)                                                                                                                                                                                gCTCGCCGCAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGACTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATAc                                                     >  1:221982/1‑250 (MQ=255)                                                                                                                                                                                    gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCa                                                <  1:193518/251‑1 (MQ=255)                                                                                                                                                                                     ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAg                                               <  1:146647/251‑1 (MQ=255)                                                                                                                                                                                                 tttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTAGATACATTCAGAATTTGGTGGCt                                   >  1:116198/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTa  <  1:30972/249‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           GCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTA  >  NC_000913/696229‑696704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑