Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	694,941	G→A	L5L (CTC→CTT)	miaB ←	isopentenyl‑adenosine A37 tRNA methylthiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	694,941	0	G	A	100.0%	47.4 / NA	18	L5L (CTC→CTT)	miaB	isopentenyl‑adenosine A37 tRNA methylthiolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/12);  total (6/12)

ACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACA  >  NC_000913/694713‑695177                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             aCCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGc                                                                                                                                                                                                                        <  1:221594/251‑1 (MQ=255)                             ttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGc                                                                                                                                                                                              >  2:115668/1‑249 (MQ=255)                                                      ccAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTAAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGCGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGCAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTtgctgc                                                                                                                                                                   >  1:122526/1‑251 (MQ=255)                                                                                          ggATTGAGCAGGTGTTCAGCAGCATCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAggg                                                                                                                               <  2:210615/251‑1 (MQ=255)                                                                                            aTTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGt                                                                                                                             <  1:88852/251‑1 (MQ=255)                                                                                            aTTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGt                                                                                                                             <  1:237610/251‑1 (MQ=255)                                                                                                 gCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTaaaaa                                                                                                                        <  1:86600/251‑1 (MQ=255)                                                                                                                            ttcttaagCCACGTGGGTCAGTTGATAGCCGTGGGAGGCATCCAGCAGATCGCCCATCTTCGTTGAATCGTACTCGTTCATATGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCCTCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCCGGGCGCCTAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCATGTCAACTTAAAGCCTATTGCTa                                                                                              >  2:126845/8‑250 (MQ=255)                                                                                                                             tattcCGCCACGTCGGTCAGTGTATAGCCGTGGGTGGCATCCAGCAGATCGGCCAGCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGTCGCATAGTGTAATGCTTTGCTGCCGTGGTGACCAGTATGCGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACGTAAAGCATATTGCTaa                                                                                             <  1:244544/248‑1 (MQ=255)                                                                                                                                   gCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAATTCCTCTAAACTTAAAGCATATTGCTAACAGgatg                                                                                      >  2:91989/1‑251 (MQ=255)                                                                                                                                                      aGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGGTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATca                                                                    <  2:255297/250‑1 (MQ=255)                                                                                                                                                         cgtcggtggCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTCTAATATGAAGTTTTTTGGTCATCGTCTTGCTCTTGCGAAATAGTATCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATcaacc                                                                 >  2:6619/5‑250 (MQ=255)                                                                                                                                                             ggtggCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACgg                                                            <  1:256628/251‑1 (MQ=255)                                                                                                                                                                ggCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGaa                                                          <  1:150613/250‑1 (MQ=255)                                                                                                                                                                     ccAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGcc                                                    <  1:115668/251‑1 (MQ=255)                                                                                                                                                                                 ccATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATAGTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCgga                                        <  2:251264/251‑1 (MQ=255)                                                                                                                                                                                                                       caagTTTTAATATGAAGTTTTTTGGGCATCGACTTGCTCTTGCGAAATATTAGCCAGGAATTCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGATCAGTATGAGCGTTATCAGCCCTTAGGGGTCAAAATCCTGTAAACTTAAAGCATATTTCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCTGAGGAATTGTCGGCGGCGCATTGTCGCTGGGGCTGGcaca  <  2:13896/248‑1 (MQ=255)                                                                                                                                                                                                                        aGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGcac   >  1:157856/1‑249 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             ACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACA  >  NC_000913/694713‑695177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑