Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,829	T→C	W139R (TGG→CGG)	djlB →	J domain‑containing protein DjlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,829	0	T	C	100.0%	43.7 / NA	16	W139R (TGG→CGG)	djlB	J domain‑containing protein DjlB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/15);  total (1/15)

GCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAAACT  >  NC_000913/677636‑678067                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 gCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTTCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCtttt                                                                                                                                                                                           <  1:164398/248‑1 (MQ=255) gCTCGCCTTTCGTGCGTTACTTGCCGCTGATAGTGATCGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATGATGGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACGGCCCATTTATCCTTCGAGGGCGTGGTGTTAGGAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTCGAAGCCTTTTta                                                                                                                                                                                         <  2:174023/250‑1 (MQ=255)    cGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATg                                                                                                                                                                                     <  1:50206/251‑1 (MQ=255)                                aGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACtt                                                                                                                                                        <  1:152120/251‑1 (MQ=255)                                 gTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTc                                                                                                                                                       <  1:34381/251‑1 (MQ=255)                                  tGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCa                                                                                                                                                                                  <  2:229918/224‑1 (MQ=255)                                                      ccTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTCCAGACCATTCTGCATCTGccc                                                                                                                                  <  2:21519/251‑1 (MQ=255)                                                          gCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTTAAGGGGGGAAATACCGGGGGAAAAGACGAAGGAAGAA‑AGGAAACACTTTTATATGTCATTGTGAAAGGGGATGATTTTTAA‑TTCTAGACCactattgatctgccccttt                                                                                                                              >  1:184700/1‑233 (MQ=255)                                                                      tcaGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCAGTGCGGAGGGGAATG‑TTTTTAACTTCCAGACCATTCTGCATCTGCTCGTTTCCGTGGAAAAt                                                                                                                   <  2:158180/250‑1 (MQ=255)                                                                                      tGCTCGATGGAGGAGATTGATGAATTACTCTGTTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGTGAAATAGACGAAG‑AAGAACTGGAATCTTTTTTATATGCCATTGCTAAGGGGAATG‑TTTTTAACTTCCAGACTATTTGGCATCTGCCCGTTGCCGTGCAAAATTACACCATCGAttgtt                                                                                                   <  2:240453/250‑4 (MQ=255)                                                                                                                                     cGCCATGAACACTGCACATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAAGG‑TTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTGACCAAATGTTCGCTCGGAGTTGGTCATCGCATCCACAATGGCTGACAt                                                   <  2:86386/251‑1 (MQ=255)                                                                                                                                                        ttATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATc                                 <  2:61923/250‑1 (MQ=255)                                                                                                                                                               tCGAGTGCGTGGTGTTATGAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCTCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGt                          <  2:88275/250‑1 (MQ=255)                                                                                                                                                                  aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGATGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTGTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTCCAGACCAGTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCAGCGATTTTTACCAAAGGTTCGCTCGGATTTGGTCAGCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGAtt                      <  1:181080/251‑1 (MQ=255)                                                                                                                                                                               attaGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGatgatg         <  1:42771/251‑1 (MQ=255)                                                                                                                                                                                      gAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAAACt  <  2:90803/251‑1 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 GCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAG‑AAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATG‑TTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAAACT  >  NC_000913/677636‑678067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑