Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,259	G→A	C175C (TGC→TGT)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,259	0	G	A	96.5%	85.2 / NA	29	C175C (TGC→TGT)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/11);  minor base C (1/0);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TACAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTG  >  NC_000913/674015‑674502                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    taCAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGAACCAGTTACGCTGCATGGTTTTAACGGAGTCTGGCCCGTGATCCAGTTTATCCAGATCGTTGAGCATCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGGTCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCACCAGCCg                                                                                                                                                                                                                                               >  1:232355/1‑251 (MQ=255)   cAACCAATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTc                                                                                                                                                                                                                                             >  2:134124/1‑251 (MQ=255)            aaGGTGTCCGGGCGGGTAGTGTAAACGGTCATCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGAGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGTCAGCAACAGCCGTCGATAACTTg                                                                                                                                                                                                                                    <  1:36193/251‑1 (MQ=255)                                         cAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTc                                                                                                                                                                                                        <  1:218553/250‑1 (MQ=255)                                                  gtCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGt                                                                                                                                                                                              <  1:68365/251‑1 (MQ=255)                                                         tCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGCACGACCGATCCAGTTACGCTGCATAGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTCCGGTCTGGTCGTTCTGGCACCAGTTGACCGc                                                                                                                                                                                       >  1:167769/1‑251 (MQ=255)                                                                               ccACGCTTTCGGAACGCCGGATCCATTTA‑GTCGGATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCa                                                                                                                                                                   <  1:138886/248‑1 (MQ=255)                                                                                    ccTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCttt                                                                                                                                                            >  1:85000/1‑251 (MQ=255)                                                                                              gACCGATACAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTCAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATCCAGc                                                                                                                                                  >  2:154008/1‑251 (MQ=255)                                                                                                  gATCCCGTTACGCTGAATGGTTTTACCGGTGTCTGGCCAGTGACCTAGGTTATCCAGAACGCTGAGCAACTCGTCAGCGTAATAAGTGATTGTGATAACCCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAACAACAGCCGTCGATAACTTGTTCGTTCGCCCGTTCGGTCTAGTCGTTCGGGCCCCCGTTGACCGCAGAAGGCTTCTTATATACCAGGCCCTTCTTATCCAGCTCgg                                                                                                                                              <  2:235062/251‑1 (MQ=255)                                                                                                           aCGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGACTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAAtt                                                                                                                                     >  1:185754/1‑251 (MQ=255)                                                                                                                  aTGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTc                                                                                                                              >  2:234511/1‑251 (MQ=255)                                                                                                                                 tCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTc                                                                                                               >  1:170386/1‑251 (MQ=255)                                                                                                                                  cTGGCCAGTGATCCATTATATCCAGATCGTTGAGCAGCTCGCCAGCGTACGCAGAGATTTTGCTACACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCCGCAACAGCCGTCGATAACTTGTTCGTTCGACAGTACGGTCTGGTCGTACGGGCACCAGCTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTATTATACAGATCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTcc                                                                                                              >  2:240001/1‑251 (MQ=255)                                                                                                                                       cAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGt                                                                                                         >  2:76237/1‑251 (MQ=255)                                                                                                                                                    tATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTCCAGGTTGcccgc                                                                                            >  2:217090/1‑248 (MQ=255)                                                                                                                                                                tGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTcc                                                                                  >  2:194008/1‑249 (MQ=255)                                                                                                                                                                        tCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTAAGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGCTAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAAcc                                                                        >  1:19726/1‑251 (MQ=255)                                                                                                                                                                                      aGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTGCAACTGTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGGTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGGCATAACCAAAGCCCAGCAttt                                                          <  1:87811/251‑1 (MQ=255)                                                                                                                                                                                         gATTTTGATAAACCACTGCGGGATCTCGTTACGTTCAACTTGGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGGCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATCCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTGCCCAACGGTAGTATTCCGGCGTACAGTTTGCCAGCTCGCGGCTCCAGTCATAACAAAAGCCCAGCAGTTTGa                                                       <  2:138157/251‑1 (MQ=255)                                                                                                                                                                                                     ccACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTc                                            >  2:239645/1‑250 (MQ=255)                                                                                                                                                                                                           cGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATAcg                                     <  1:27057/251‑1 (MQ=255)                                                                                                                                                                                                              gATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATAcgcg                                   >  1:167048/1‑250 (MQ=255)                                                                                                                                                                                                                    tttACGTTCAACTTTGGTATACCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTAAAGGTTGCAAGCTCGCGGCTCCAGTCATAACCAACGCCAAGCGTTTTGAGCTGGTTTTTCATATACGCGAtgttgt                            >  2:264154/1‑251 (MQ=255)                                                                                                                                                                                                                       aCGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCGCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCg                          <  1:158886/250‑1 (MQ=255)                                                                                                                                                                                                                        cGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGt                         >  1:164070/1‑250 (MQ=255)                                                                                                                                                                                                                                 ttGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACg                <  1:234511/250‑1 (MQ=255)                                                                                                                                                                                                                                        cGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCgg         <  1:154008/250‑1 (MQ=255)                                                                                                                                                                                                                                              gcCAGCAACAGCCGTCGATAAATTGGTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTGGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTCCGTCCACGGTGCCGGAGCGgtg  >  2:211707/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TACAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTG  >  NC_000913/674015‑674502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑