Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,469	C→T	A711A (GCC→GCT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,469	0	C	T	100.0%	40.0 / NA	15	A711A (GCC→GCT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/11);  total (4/11)

ATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATC  >  NC_000913/2224‑2692                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                atgaAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCAGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGCTCTTTCTGGAATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAACTTGAACCTGTGCTGCCCGCAGCGTTTAACGCTGAggg                                                                                                                                                                                                                            >  1:176925/1‑251 (MQ=255)     aGTTCTCCGGTATTCTTGCAGGTTCGCTTTCGTATATCTTCGTCAAGTTAGACGAAGGCATGAGTTTCTCCTAGTCGACTACTCTTGCGCGGGAAATGGGTTATACCGAACCGGAGCCGCGAGATGATCTTTCTGGTATGGATTTAGCGCGTAAGCTATTTATTCTCGCGCGTTAAACGGGTCGTTAACTTGAGCTGGCGGATATTGAAATTGAACCTGTGCGTCTCGCACAGTTTAGCGCTGAGTGTGAt                                                                                                                                                                                                                        <  2:159352/251‑1 (MQ=255)           ccgtcagtctttcaGGTTCGCGTTCGTATATCGGCGGCAAGTTAGACGAAGGCATGAGGTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCTGATCCGCGAGATGATCGTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGTAACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCGGCCCGCAGATTTTAACGCTGAGGGTGATGtt                                                                                                                                                                                                                     <  1:184166/241‑1 (MQ=255)                                      atatatTCGGCAAGTTAGACTAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTAGACCGAACCGGATCCGCGAGATGATCTTGCTGGTATGGATGTAGCGCGGAAGCTATTGATTCGCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCTCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTc                                                                                                                                                                                       <  2:186638/251‑1 (MQ=255)                                                                      ttCTCCGTGGCGACTACGCCGGTGCGGGAAATGGGTTATACCGAACAGGATCCGCGAGATGATCTTTCTGGTAGGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCTTGTCGAAgg                                                                                                                                                        <  2:217794/250‑1 (MQ=255)                                                                          ccGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGTGACGTGAACTTGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGAAGATCACTTTGCCGCGCCCGTGTCGAAGGcc                                                                                                                                                      <  2:161953/248‑1 (MQ=255)                                                                                                   aaTGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTTCGCTATg                                                                                                                          <  2:245909/251‑1 (MQ=255)                                                                                                           ataCCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGGTTTGCGCTATGTTGGCAat                                                                                                                  <  1:193031/251‑1 (MQ=255)                                                                                                                            cgAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTTGCAATATTGATGAAGATggtgc                                                                                                 <  1:212769/251‑4 (MQ=255)                                                                                                                             gagaTGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATggtgcc                                                                                                <  2:182701/251‑5 (MQ=255)                                                                                                                                     cTTTCTGGTATGGATGTAGCGCGTAAGCTATTGCTTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGCAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGGGCCTGCCGCGt                                                                                        >  2:263224/1‑251 (MQ=255)                                                                                                                                             tATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGGGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGACACAGCTCGACGATCGCTTTGCAGCGAGCGAGGCGAAGGCCCGTGATGAAGGAAAAGTTTGGAGCTATGTGGGCAAGATTGAGGAAGATGGAGCATGCCGCGTGAAGaatg                                                                                >  1:149474/1‑248 (MQ=255)                                                                                                                                                                           cgctcgTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTc                                                   <  1:264836/250‑1 (MQ=255)                                                                                                                                                                                                     aGCTGGCGGATATTGAGATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATAGTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTg                        <  2:26864/251‑1 (MQ=255)                                                                                                                                                                                                                             cTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGAGGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATc  >  1:249246/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                ATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATC  >  NC_000913/2224‑2692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑