Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,382	A→G	K682K (AAA→AAG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,382	0	A	G	100.0%	60.3 / NA	21	K682K (AAA→AAG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/14);  total (7/14)

AAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTG  >  NC_000913/2140‑2614                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         aaaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGGAGCGCGTAAGCTATTGAt                                                                                                                                                                                                                                  <  1:208289/251‑1 (MQ=255)      ccTCTATGACACCAACGTTGGTGCTGGATTACCGGTTATTGAGAATCTGCAAAATCTGCTCAATGCTTGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCATGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGATATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTctcg                                                                                                                                                                                                                             <  2:451/251‑1 (MQ=255)                acCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCGGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAAc                                                                                                                                                                                                                   <  1:137905/251‑1 (MQ=255)                               ggATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTgg                                                                                                                                                                                                     >  1:26864/1‑250 (MQ=255)                                        gTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCgg                                                                                                                                                                                            >  1:160886/1‑250 (MQ=255)                                                gaACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCGTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATattgaaa                                                                                                                                                                                   <  1:148335/251‑1 (MQ=255)                                                      gCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAAc                                                                                                                                                                             >  1:215709/1‑251 (MQ=255)                                                                                     atgaAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCAGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGCTCTTTCTGGAATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAACTTGAACCTGTGCTGCCCGCAGCGTTTAACGCTGAggg                                                                                                                                              >  1:176925/1‑251 (MQ=255)                                                                                         aGTTCTCCGGTATTCTTGCAGGTTCGCTTTCGTATATCTTCGTCAAGTTAGACGAAGGCATGAGTTTCTCCTAGTCGACTACTCTTGCGCGGGAAATGGGTTATACCGAACCGGAGCCGCGAGATGATCTTTCTGGTATGGATTTAGCGCGTAAGCTATTTATTCTCGCGCGTTAAACGGGTCGTTAACTTGAGCTGGCGGATATTGAAATTGAACCTGTGCGTCTCGCACAGTTTAGCGCTGAGTGTGAt                                                                                                                                          <  2:159352/251‑1 (MQ=255)                                                                                               ccgtcagtctttcaGGTTCGCGTTCGTATATCGGCGGCAAGTTAGACGAAGGCATGAGGTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCTGATCCGCGAGATGATCGTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGTAACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCGGCCCGCAGATTTTAACGCTGAGGGTGATGtt                                                                                                                                       <  1:184166/241‑1 (MQ=255)                                                                                                                          atatatTCGGCAAGTTAGACTAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTAGACCGAACCGGATCCGCGAGATGATCTTGCTGGTATGGATGTAGCGCGGAAGCTATTGATTCGCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCTCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTc                                                                                                         <  2:186638/251‑1 (MQ=255)                                                                                                                                                          ttCTCCGTGGCGACTACGCCGGTGCGGGAAATGGGTTATACCGAACAGGATCCGCGAGATGATCTTTCTGGTAGGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCTTGTCGAAgg                                                                          <  2:217794/250‑1 (MQ=255)                                                                                                                                                           tctcCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTctcgcc                                                                                                                                                                                                                           >  2:146238/1‑103 (MQ=255)                                                                                                                                                           tctcCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTctcgcc                                                                                                                                                                                                                           <  1:146238/104‑2 (MQ=255)                                                                                                                                                              ccGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGTGACGTGAACTTGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGAAGATCACTTTGCCGCGCCCGTGTCGAAGGcc                                                                        <  2:161953/248‑1 (MQ=255)                                                                                                                                                                                       aaTGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTTCGCTATg                                            <  2:245909/251‑1 (MQ=255)                                                                                                                                                                                               ataCCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGGTTTGCGCTATGTTGGCAat                                    <  1:193031/251‑1 (MQ=255)                                                                                                                                                                                                                cgAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTTGCAATATTGATGAAGATggtgc                   <  1:212769/251‑4 (MQ=255)                                                                                                                                                                                                                 gagaTGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATggtgcc                  <  2:182701/251‑5 (MQ=255)                                                                                                                                                                                                                         cTTTCTGGTATGGATGTAGCGCGTAAGCTATTGCTTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGCAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGGGCCTGCCGCGt          >  2:263224/1‑251 (MQ=255)                                                                                                                                                                                                                                 tATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGGGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGACACAGCTCGACGATCGCTTTGCAGCGAGCGAGGCGAAGGCCCGTGATGAAGGAAAAGTTTGGAGCTATGTGGGCAAGATTGAGGAAGATGGAGCATGCCGCGTGAAGaatg  >  1:149474/1‑248 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         AAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTG  >  NC_000913/2140‑2614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑