Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	578,427	G→C	T107T (ACG→ACC)  R34P (CGG→CCG)	rzpD → rzoD →	putative prophage endopeptidase RzpD putative prophage lysis lipoprotein RzoD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	578,427	0	G	C	100.0%	31.1 / NA	13	T107T (ACG→ACC)  R34P (CGG→CCG)	rzpD rzoD	putative prophage endopeptidase RzpD putative prophage lysis lipoprotein RzoD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (3/10);  total (3/10)

GACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACGGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGATAGAGCTGACCATATCGATGGGCAACTCATGCAATTATTTTGAGCAATACACACGCGCTTCCAGCGGAGTATAAATGCCTAAAGTAATAAAACCGA  >  NC_000913/578263‑578661                                                                                                                                                                     |                                                                                                                                                                                                                                           gACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGTTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATc                                                                                                                                                        <  1:21862/249‑1 (MQ=255)    aTGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGCTGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCCCCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGACCGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTAt                                                                                                                                                   >  1:131866/1‑251 (MQ=255)                  gCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTAGGCAAAAACAACTgg                                                                                                                                     <  1:186070/251‑1 (MQ=255)                     tGATGTTGCTGCGCACGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAACACAACTgga                                                                                                                                    >  1:40847/1‑249 (MQ=255)                       aTGTTACTGCGCTCGATGCAACATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCCGCCACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTggaagg                                                                                                                                 >  2:59223/1‑250 (MQ=255)                           gtctgcCCTCAATGCAAAATGCACGAAGGATTTAGCTAATGCAAAACCTGAAAATGAGGCTCGGCGTGATGAGGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGGCGGTCAGTCAGTGCGTGAAGCCACCACCGCCGCAGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACATCGCTGAACGGGATTATTACACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAAccc                                                                                                                            <  1:257489/249‑1 (MQ=14)                                     gATGAAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAAGGATGCTCTGCGTGAGTATGTTGCCGCGGGTCGTCGTCGGTTGCACATCAAAGCATTCTGTCAGTCAGTGCGTGAAGCCCCCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAACCCAGAAGTatat                                                                                                                  <  2:166469/251‑1 (MQ=17)                                         aaaaaTACCCGAAGGTGTTATCGGACGCTAAAGCGAAAAAGGATGCTCTGCGTGAGGATGTTGCCGCTGGTCGCCGTCGGTTTCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAACCCAGAAGTATATTAAt                                                                                                              <  1:144205/250‑1 (MQ=17)                                                      ggAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGAt                                                                                                 <  1:240903/251‑1 (MQ=255)                                                        gtttAGCTGATGTTAAAGCTGAAAAGGAGTCTCTGTGTGATGTTGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGCAAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACGGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGATag                                                                                               <  2:131866/249‑1 (MQ=18)                                                                      aaaGCGGAAAATGATGCTCGTCGTGATGATGTTCCCGCGTGTCGTCGTCGTTTGCACATCAAAGCAGTCTGGCAGGCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGATAGAGTTGCCCATATCg                                                                                 <  1:14246/251‑1 (MQ=18)                                                                                                                                         ccTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGATAGAGTTGCCCATATCGATGGGCAACTCATGCAATTAGTGTGAGCAATACACACGCGCTTCCAGCGGAGTATAAATGCCTAAAg              <  2:46932/250‑1 (MQ=255)                                                                                                                                                      gCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGATAGAGTTGCCCATATCGATGGGCAACTCATGCAATTATTGTGAGCAATACACACGCGCTTCCAGCGGAGTATAAATGCCTAAAGTAATAAAACCGa  <  2:17774/250‑1 (MQ=255)                                                                                                                                                                     |                                                                                                                                                                                                                                           GACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACGGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAAAACAACTGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGATAGAGCTGACCATATCGATGGGCAACTCATGCAATTATTTTGAGCAATACACACGCGCTTCCAGCGGAGTATAAATGCCTAAAGTAATAAAACCGA  >  NC_000913/578263‑578661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑