Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	292,019	C→T	L174L (CTA→TTA)	yagJ →	protein YagJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	292,019	0	C	T	100.0%	94.0 / NA	32	L174L (CTA→TTA)	yagJ	protein YagJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/17);  total (15/17)

GACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCAT  >  NC_000913/291785‑292261                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   gACGAAAGCGGGAACTATCCGGCTCGGTTGAACCATGGAGAATCCAAGGTTTGTGAGATCGAAACCATACGTGAAGGATTTGCTTTCTGGCATCGTAATCCACAGTACACAGTGCAGTCGTCACTGAGAATCGCTTATGTTGTAGCTGAACAGTACAAGATGGTTCTTCCCGATTTCCTGGTCTTTGCCGAACAGGAGGGTAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATg                                                                                                                                                                                                                                     <  1:267275/250‑1 (MQ=255)             aaCTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACt                                                                                                                                                                                                                        <  2:17666/251‑1 (MQ=255)                   ccGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAgg                                                                                                                                                                                                                  >  1:102039/1‑251 (MQ=255)                      gCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACt                                                                                                                                                                                                               <  2:206920/251‑1 (MQ=255)                                      gggAAACCAAGGTTTTTAAGATCGAAAACAAACGTGAAGCAATTAATTTCTGGTATCGTAATCCACAGTAAACAGGGCAGTAGTCACTGGGAATCGCTTATGTTACAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGCTGGCAAAATGTTTGTGGAACTGGTAGATCCACATAGCTTACATCTGGCTGCTGCTTTGCCCAACCTGGAAGGACTTGCGCTATATGcagac                                                                                                                                                                                               >  2:159549/1‑247 (MQ=255)                                         aaaCCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGAATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAAcat                                                                                                                                                                                            >  1:43590/1‑251 (MQ=255)                                           aCCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAAcatca                                                                                                                                                                                          <  1:37917/251‑1 (MQ=255)                                                       gagaTCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCTCAGTACACAGGGCAGGGGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGGGCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCGCATAGCTTACATCTGGCTGATGCTTTGCCTAAACTGGAAGGACTTGTGCTATATGCCGAACATCATTCGGATGCTTa                                                                                                                                                                              <  2:214379/251‑1 (MQ=255)                                                          aTCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTAc                                                                                                                                                                             >  1:177582/1‑249 (MQ=255)                                                          aTCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTAc                                                                                                                                                                             >  2:98592/1‑249 (MQ=255)                                                            cGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGCAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGCACATCATTCTGATGCTTACAGGc                                                                                                                                                                         >  2:194081/1‑251 (MQ=255)                                                                     aCGTGAAGGATTTGCTTTCTGGTATCGTCATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGATGATGCTTTGCCCAAACTGGAAGGACTAGCGCTATCTGCCGCACATCATTCTGATGATTACAGGcgaatcgaat                                                                                                                                                                >  2:55858/1‑251 (MQ=255)                                                                            ggATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGCACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATCTGCCGAACATCATTCTGATGCTTACAGGCGCATCGAATCTGTCGc                                                                                                                                                         >  2:19579/1‑251 (MQ=255)                                                                              aTTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCg                                                                                                                                                       <  2:177582/251‑1 (MQ=255)                                                                                            aTCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACAGCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATGCTGAAGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAggggaa                                                                                                                                          <  2:76093/250‑4 (MQ=255)                                                                                                      acaGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAAGCTGTCGCCGAAGTAAAGGTTAAATTACGAgtgt                                                                                                                               <  1:213333/251‑1 (MQ=255)                                                                                                         gtacatagtgtaGTCGTCACTGGGAATCGCGTATGTTGAAGCTGAACAGTACAAGATTGTTCGTTCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGTTATATGCCGAACATCAGTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAg                                                                                                                            <  1:121147/240‑1 (MQ=255)                                                                                                          tACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGtt                                                                                                                              >  1:161658/1‑248 (MQ=255)                                                                                                           acacAGGGCAGGCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGAt                                                                                                                          <  2:150407/251‑1 (MQ=255)                                                                                                            cacaGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGAtt                                                                                                                         >  2:104103/1‑251 (MQ=255)                                                                                                                                  gtCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTACTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAACAGGCAGGATGTGCAgg                                                                                                    >  2:41925/2‑250 (MQ=255)                                                                                                                                    cGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATg                                                                                                 >  1:51002/1‑251 (MQ=255)                                                                                                                                           gTTGAAGCTGAACAGTACAAGACTGTTCGTCCCGATTTCATGTTCTTTGCCGAACAGGATGGCAAAATGGATGTGGCCCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGACTCGAATCTGTCGCCGCAGCAAAGGGTAACTTCCCAGTGTTAGATTTGAAACGGCAGGATGAGCCGGATCCTGTTg                                                                                           >  2:35593/1‑250 (MQ=255)                                                                                                                                               aaGCTGAACAGTACAAGATTGTTCGTTCCGATTTCCTGGTCTTTGCCGAACAGGATGGCAAAAGGGTTGTGGACCTGGTAGATCCTCATAGCTTACATCTGGCTGATGCTGTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCTTCGAAGTAAAGGGTAAATTTCGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGTTGTTGCtccc                                                                                      <  1:259432/251‑4 (MQ=255)                                                                                                                                               aaGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGTAAAATGGTTGTGGACCTGGGAGATCCACATAGCTTCCATCGGGCTGATGCGTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTTAAACGGCAGGATGTGCAGGAGGCTGTTGCTAcc                                                                                      <  1:55858/251‑1 (MQ=255)                                                                                                                                                        aGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAAt                                                                             <  1:98592/251‑1 (MQ=255)                                                                                                                                                                        tCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGTTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAACCTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTTCTACCGCTGAAAATGCAGAAACGTTAttt                                                              >  2:245429/1‑250 (MQ=255)                                                                                                                                                                                         ttGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGTAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCtga                                             <  1:35593/250‑1 (MQ=255)                                                                                                                                                                                                  aGGATGGCCAAATGGCTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGCAGGCCTTGAGCTATCTGCCGAACATCATTCTGATGTTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCCGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATc                                     >  2:157199/1‑249 (MQ=255)                                                                                                                                                                                                                   gtgGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTg                  <  2:224540/251‑1 (MQ=255)                                                                                                                                                                                                                           ggTAGATCCACATAGCTTACATCTGGCTGAGGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGGTGAAAATGCAGAAACGTTATTTAGGAGTGGACATGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGa          <  1:251024/251‑1 (MQ=255)                                                                                                                                                                                                                                   cacaTAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGTTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCAt  <  1:157199/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCAT  >  NC_000913/291785‑292261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑