| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 271,817 | G→A | pseudogene (54/427 nt) | insN → | IS911A regulator fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 271,817 | 0 | G | A | 95.5% | 61.8 / ‑6.0 | 22 | pseudogene (54/427 nt) | insN | IS911A regulator fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (12/9); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTC > NC_000913/271572‑272042 | cggtggcagatgccgcgaaagctatggatgttggcctttccacaatgacaagatgggtcaaacaacttcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGagcgca > 2:18001‑M1/191‑250 (MQ=255) aagatgggtcaaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCagcagc > 1:80774‑M1/142‑251 (MQ=255) ggtcaaacaactgcgtgatgagagtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgcaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCACTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGATCATCGCAGCAGCTAc < 1:45779‑M1/113‑1 (MQ=255) aactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaaacgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGaa > 2:208541‑M1/128‑251 (MQ=255) atgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTcc > 1:260631‑M1/119‑251 (MQ=255) gtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgacatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGaaa < 1:113516‑M1/138‑1 (MQ=255) gtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGaaa < 1:41181‑M1/138‑1 (MQ=255) gggcaaaacaccaaaagcctctccgataaaaccagaacaaatcgaaatacgtaagctgaggaaaaagatacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATACTCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGAAGCTACAGATACTGGAAAAACCGtcctgaagaacc < 2:109730‑M1/142‑6 (MQ=255) tctccgataacaccagaacaaatcgaaatacgtaagctgaggcaaaagctacagcgcattgaagtagagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCACTGAACAGTTCTCGATAATCGGGCAACTCAGGGCGCATTATCCTGTGGTAACACTCTGCCGTGTGATCGGAGTGCATCGCAGCAGCTACAGATACTGGAAAAGCCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTAt < 1:64543‑M1/162‑1 (MQ=255) gataacaccagaacaaatcgaaatacgtaagctgaggaaaacgctaccacgcattgaaatggagaatgacatattaacaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGc < 1:239261‑M1/167‑1 (MQ=255) agaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTa > 1:87414‑M1/76‑251 (MQ=255) agaacaaatcgaaatacggaagcagaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGTAAAAACCGCCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTa > 2:122177‑M1/76‑251 (MQ=255) gaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCt < 1:46444‑M1/111‑1 (MQ=255) gaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCAGCGCAGCAGCt > 2:46444‑M1/75‑185 (MQ=255) aatacgtaagcggaggaaaaagcgacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCTCTCTTGATGTCAGACTCCCTGAACAGTTCTCTATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTTGAAAAACCGTCCTGAAAAGCCATACGGCAGACGGGCCGTAGTACGCAGTCTGttacttgagctacat > 2:171125‑M1/64‑244 (MQ=255) aaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTTGGCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATGCTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGccacggttcg > 1:89523‑M1/46‑247 (MQ=255) aaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGccacggttcg < 1:2174‑M1/206‑5 (MQ=255) aaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGccacggttcg > 2:2174‑M1/46‑247 (MQ=255) acgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGc < 1:59693‑M1/214‑1 (MQ=255) ttgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCaaga < 1:41345‑M1/218‑1 (MQ=255) aaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAacccg > 1:109730‑M1/10‑249 (MQ=255) ccGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCCCACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTACTGAAAACCCAGCCGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCAACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTAcc > 1:49575/1‑251 (MQ=18) gTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACAAGACGGCAGACGGGCTGTATTACGCAGTCAGGGACTTGAGCTACATGACATCAGCAACGGATCGGCCGAAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGCTGGGACGCTGGCTTGCTGGCAgggtc > 1:225738/1‑246 (MQ=17) | GGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTC > NC_000913/271572‑272042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |