Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	154,182	C→T	L415L (CTG→CTA)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	154,182	0	C	T	100.0%	58.2 / NA	21	L415L (CTG→CTA)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/11);  total (10/11)

CGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCAT  >  NC_000913/153937‑154414                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         cgcttagATTTCGGTTGAAGATCTTGATCTAGATGGTTCACTTCATCAATTCGAGTTAGTGCATCATTAAGACCAAGGTACTTCTGTGTCGAAAAGCGATAGGCCGGGATATTCAGTGAATTTCTTGTTTCTTAGAATAACTTGTTCCGGGAAACACGATAACTTTGCCCATGGTATGTTTTATCATCCGGGATACGAACATTGGCATGAGTCACA‑TCGATAAATAATGCACCAACTGAAGTATTTAGAcc                                                                                                                                                                                                                                     >  2:188895/7‑251 (MQ=255)          ttCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTa                                                                                                                                                                                                                             >  1:108026/1‑250 (MQ=255)              gTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACaaa                                                                                                                                                                                                                        <  2:86053/251‑1 (MQ=255)               ttCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCACGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAAc                                                                                                                                                                                                                       >  2:250228/1‑251 (MQ=255)                   aGATCTTGTTCGGGATGTTACACTTCATAAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATACTGTGTCGCATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTTTTCCAGGAAACACGATAACTTTGCCCATGGTATGTTTTATCATACGTGATACGAACATTAGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAACCCAGc                                                                                                                                                                                                                   >  1:38130/1‑251 (MQ=255)                                gATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGttatta                                                                                                                                                                                                      <  1:84231/251‑1 (MQ=255)                                           tcatcaATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCt                                                                                                                                                                                            <  1:160174/250‑1 (MQ=255)                                                                           aaGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCa                                                                                                                                                           <  1:204593/251‑1 (MQ=255)                                                                                                   aTAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGaa                                                                                                                                   <  1:216713/251‑1 (MQ=255)                                                                                                                      gAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCTCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAATACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATGCCGGTATAACCCGTCAGATAGTTATTCAGGCGGTAGTAGTAGCGTGCTTGAAATAAATTAGGCGCATCCTg                                                                                                                <  2:111802/251‑1 (MQ=255)                                                                                                                                   cttcGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCtttt                                                                                                   >  2:183058/1‑251 (MQ=255)                                                                                                                                     tcGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCttt                                                                                                    <  1:3402/248‑1 (MQ=255)                                                                                                                                                                     ttGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGCGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACAGCCGCTAATATCCCAACGTCCa                                                                  >  2:243609/1‑250 (MQ=255)                                                                                                                                                                             ggTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAAGGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCACCCTGAATATCATCTTTTAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAgg                                                          <  1:250228/250‑1 (MQ=255)                                                                                                                                                                                            ccGGGATACGAACATGGAAATGA‑TTACAATCAAAAAAAAATGCACCAACTGAAGAATTTAGCCCAAGCCCTAACAAACCAGTGGTATATTTATTATCGTTTATCTGATTACTGATATACCCCGTCAAATAGTTTTTCAGGCCGTAGTAGTATCTGTTTTAAAAAAAATGGGGCTAACCCTAATTACCATCGTTAAGGCCCGGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaaca                                          <  2:235919/251‑1 (MQ=255)                                                                                                                                                                                                 aTACGAACATTGGAATGAGTCACA‑TCGTAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACgg                                      >  1:202478/1‑250 (MQ=255)                                                                                                                                                                                                     gAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCCTCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATg                                   >  1:123507/1‑249 (MQ=255)                                                                                                                                                                                                        cATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGaa                               >  1:30221/1‑250 (MQ=255)                                                                                                                                                                                                         aTTGGAATGAGTCACA‑TCGAAAGAAAAGGCACCAACTGAAGTATTTAGACCAAGACCAAACAAACCAGCGGTATATTTATTATCGGTTATCTGAATACCGGAATAACCCGTCAGATAGTTATTCAGGCCGTAGGATTAGCTTGCTTGAAATAAATTATGCTCATCCTGAATATCATCTTTTAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAg                             <  1:183058/251‑1 (MQ=255)                                                                                                                                                                                                                            aaaagaaaATGCCCCAACTGATGTTTTTAGACCAAGACCTAACAAACCAGGGGTAAATTTATTATCGGTTATTGGAATCCCGTTATAACCCGTCAGATATTTATTCAGGCCGGATTAGTAGCTTGCTTAAAATAAATTAGCCTCTTCCTGAATATCAGTTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCt           <  2:108026/250‑1 (MQ=255)                                                                                                                                                                                                                                     tGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGCCTAGTTATAATCGGATATCTGAATACCGGTATAACCAGTCAGATAGCTCTTCAGACCGTAGTCGAAGATTGCTTGATATAAATTAGGATCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCACCGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGCAAGGTTGCGAGAATGTCCGCTTTGAGCCAt  >  1:18982/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         CGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACA‑TCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCAT  >  NC_000913/153937‑154414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑