Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	81,102	G→A	S286L (TCG→TTG)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	81,102	0	G	A	96.9%	86.2 / NA	32	S286L (TCG→TTG)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/17);  minor base C (1/0);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCGA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCG  >  NC_000913/80861‑81309                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                 ccATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAAAGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCCGCGAAAGGATTTGTGCAATCGGGTTGGCGATGATTTTT‑CCTGCGATATCTGGTGCCCA‑Gccgcccc                                                                                                                                                                                                        >  2:55210/1‑250 (MQ=255)                 tCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTcc                                                                                                                                                                                        >  1:204210/1‑251 (MQ=255)                     cTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCCTACAGTCCaaa                                                                                                                                                                                     >  1:27495/1‑250 (MQ=255)                        cATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑Gccgcccg                                                                                                                                                                                                        <  1:16736/228‑1 (MQ=255)                        cATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑Gccgcccg                                                                                                                                                                                                        >  2:16736/1‑228 (MQ=255)                                           gCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGTGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCCGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCgg                                                                                                                                                              >  1:34025/1‑251 (MQ=255)                                                tttCACCGGTACGAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAAGGCTGTCTTCTAAGGCGCGGTTAATGGCGCGTTAAAGGGCGAAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGGGCCAGCGAAAGGATTGGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGTCCGTCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAgg                                                                                                                                                          <  1:250443/250‑1 (MQ=255)                                                       gtactcaccgcggccaggcccgcgtgCTAAACCCCCGGTGCAAAGCCCTTGTTCAAACGCGCGGTTAATGCGGCGTCAAATGGCGCAAGCCGCATCAGCGGCCTCAAGGTTGTAACGCAGCAGCAGTCCCAGCGAAAGGATTTGTGCAATCGGGTGGGCGATGTTTTGG‑CCGGCGATATCTGGTGCAAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATcc                                                                                                                                                  <  2:9552/226‑1 (MQ=255)                                                       ggTACTAACGGCGGCAGCCACACGGGCTCTATTCCCGATGCGAAAGACTTCTTCTAATTCGCGGGTCAAGGCGCGATCCATGGCGCAAGCCGCATCATCGGCATCCAGCCTGTAACGCAGCAGCAGTGCCAGCGAAAGGCTTTGTGCAATCGGGATGACGATGATTTTG‑CCTGCGATATCTGTTGCCAC‑GACGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCCGAAGCAAACATcc                                                                                                                                                  >  2:24318/1‑251 (MQ=255)                                                             atcggcggCCGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTCCTAATGCGCGGTTAATGGCGCTTTCAATGGCGCAAGCCGCATAATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGTAAGGCAACATCCCCATCg                                                                                                                                            <  1:72521/249‑1 (MQ=255)                                                                               ggctaaccccccGGTGCGAATGCCTTCTTCTAAGGCGGGGTTAATGAGGCGTCCAAGGGCGCAAGCCGCATCTTGGGCATCCAGGCTGTAACGCAGCAGCAGGGCCGGCGAAAGAATTTGGGCAATCGGGTTGGCGATTTTTTTG‑CTTGCGATAGCGGGTGCCAG‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTgcgc                                                                                                                          <  2:231312/243‑1 (MQ=255)                                                                                            gTGCGAAGGCTTTCTGCTAATGCGCGGTAAATGGCTCTTTCAAGGGCGCACGCCGCATCATCGGCATCCAGGCGGTACCTCAGCTGCAGTGCCAGCGAAAGGTTTTGTGCAATCGGGTTGGCGATGTTTGTG‑CCTGCGATATCGGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCAGTGCGCACTCGTcagaca                                                                                                              <  2:129383/250‑1 (MQ=255)                                                                                                gAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATg                                                                                                         >  1:26938/1‑251 (MQ=255)                                                                                                             tactGCGCGGTTAATGGAGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGACAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCCAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGt                                                                                            >  2:95699/4‑251 (MQ=255)                                                                                                                            ggCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCCGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGa                                                                              >  1:164573/1‑250 (MQ=255)                                                                                                                             cctcgtccAATGGCCCAAGCCGCACCATCGGCATCCAGGCTGTAACGTAGCAGCAGTGCCGGCGAAAGGATTTGTGCAATCGGGTTGCCGCTTTTTTTG‑CCTGCGATATCTGGTTCCAA‑CCCGCCCGCCTGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCGTGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAAc                                                                            <  1:244951/244‑1 (MQ=255)                                                                                                                                   taaaTGGCGCAAGCCGAAGCAGCGGCATCCAGGCGGTAACGCAGCATGAGTGCCAGCGAAAGGATTTGGGCAATCGGTTGGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCaa                                                                       <  2:26938/248‑1 (MQ=255)                                                                                                                                    aaaTGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCTATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCaaa                                                                      <  1:116558/249‑1 (MQ=255)                                                                                                                                       gggCGCAACCCGCATCAGCGGCATCCAGTCGGTAACGCAGCAGCAGTCCCAGCGAAAGGATTTGGGCAAGCGGGGTGGCGATTTTTTTG‑CCGGCGATATCTGGTCCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACtgt                                                                  <  2:234512/250‑1 (MQ=255)                                                                                                                                        ggCGCAAGCCGCATCAGCGGCACCCAGGCTGTAACGCAGCAGAAGGGCCAGCAAAAGGATTTGTGCAAGCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACTTCAAACtgtg                                                                 <  2:27495/251‑1 (MQ=255)                                                                                                                                          cgcAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGAt                                                               <  1:52912/251‑1 (MQ=255)                                                                                                                                            cAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACCGCAGAACGTCAAACTGAGATgg                                                             >  1:28190/1‑251 (MQ=255)                                                                                                                                                      cattggCACCCAGGCTGTAACGCAGCAGCGGTGCAGGGGAAAGGATGTGTGCAAGCGGGTTGGCGAT‑TTTTTGTCCGGCGATATCTGGTGCCAC‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATc                                                   <  2:204210/247‑1 (MQ=255)                                                                                                                                                         cGGCATCCAGGCTGTAAAGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACCGGTTGGAGCACAGCAGAACGTCAAACTGTGCTGGCTCTTTAATCAGc                                                >  2:125329/1‑251 (MQ=255)                                                                                                                                                                cAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg                                         >  1:193272/1‑251 (MQ=255)                                                                                                                                                                   gCTGTAACGCAGCAGCAGTGCCAGCGAAAGTATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtgg                                      <  1:3196/251‑1 (MQ=255)                                                                                                                                                                      gTAACGCAGCAGCAGTGCCAGCGAAAGGATTGGTGCAAGCGGGTTGGCGAGTTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCAGACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGt                                   <  1:128509/251‑1 (MQ=255)                                                                                                                                                                          cgcagcacattcgccggcaaaaGGATTTGTGCAACCGGTTTGGCGATTTTTTGG‑CCTGCAATCTCTGGTGCCAA‑GCCCCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc                               <  2:193272/232‑1 (MQ=255)                                                                                                                                                                                agcagTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                          >  1:102348/1‑250 (MQ=255)                                                                                                                                                                                            gAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAAACTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTAAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGcc               >  1:129995/1‑249 (MQ=255)                                                                                                                                                                                               aGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGCTATCTGGTGCCAA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGCGCCCGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAAAAGGTTGTAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGCGGAGTTGTCGATGTACATATGCGCCAGTTc          >  2:18925/1‑251 (MQ=255)                                                                                                                                                                                                       ggcactggggTTGGCGATGTTTTTG‑CCGGCGATCACTGG‑GCCAAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTGCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCg  <  2:47689/244‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                 CCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTG‑CCTGCGATATCTGGTGCCGA‑GCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCG  >  NC_000913/80861‑81309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑