Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,635	C→T	intergenic (‑85/+200)	araD ← / ← araA	L‑ribulose‑5‑phosphate 4‑epimerase AraD/L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,635	0	C	T	100.0%	95.1 / NA	31	intergenic (‑85/+200)	araD/araA	L‑ribulose‑5‑phosphate 4‑epimerase AraD/L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/15);  total (16/15)

GTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGC  >  NC_000913/66389‑66874                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCa                                                                                                                                                                                                                                             <  2:80100/251‑1 (MQ=255)            tGACGCCGTAATCGACGCCGGAAGGTTTGATCCCAGAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGCGACCCGGTTGTGTTTTGGCAGAGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGGTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGAGCCAGCGTCGTATCAGGCGTTGAATg                                                                                                                                                                                                                                  >  2:29025/1‑251 (MQ=38)                     aaTCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATgcg                                                                                                                                                                                                                         >  1:240248/1‑251 (MQ=255)                         gACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCttt                                                                                                                                                                                                                     <  1:189172/251‑1 (MQ=255)                                   ggTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCgg                                                                                                                                                                                                           <  1:114268/251‑1 (MQ=255)                                     tttGATAACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTCATAACTGGCGTTTGAGATCTTCTACCATGTTGCCTCCTTCGTGCCGGATGCGCTTTGCTTATCCGCCCTCCAACATCGCCGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGcc                                                                                                                                                                                                         >  2:244217/1‑251 (MQ=37)                                       tGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGCGCGAGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCAGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGGCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCt                                                                                                                                                                                                        >  2:12966/1‑250 (MQ=255)                                             aaaaGACGTCGCGCGAGCGATCAACGGCGCCGATGTTGTCCCATGTGAGCGTGACCAGGTGGTGTTTGTGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCGTCTAACATGTTGACTCCTGCGTGCCGGATGCGCTTTGCGTAGCCGGCCTACAAAATCGCAGCGTGTAGGCCTGAGAAGACGCGCCAACGTCGTAACAGGCGTTGTATGGCGGATGCTCTATGCTTATCCgtcgtataaaat                                                                                                                                                                                                 <  2:254940/250‑12 (MQ=255)                                              aaaGACGCCGCGCTCGCGATCAACTGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTGGTGATTTGGCAGCTCCAGGTTGGCTTCTAATACCTGGCGTTTTAGATCTTCTAACATGTTGACTCCTTCGTGCGGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTAGCCGGCCTACAAAATc                                                                                                                                                                                                <  2:47545/251‑1 (MQ=25)                                                 gACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGGGAGCGTGACCAGGGTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGACTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGc                                                                                                                                                                                              >  2:207724/1‑250 (MQ=255)                                                    gccgcGCTCGCGATCAACGGCGCTGCCGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTAGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAgcg                                                                                                                                                                                          >  2:185764/1‑251 (MQ=255)                                                                        cgcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGAcgcg                                                                                                                                                                      <  1:48330/251‑1 (MQ=255)                                                                          cTGACGTTGCCCCCTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCAAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCAGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGcc                                                                                                                                                                    >  1:174543/1‑251 (MQ=255)                                                                             aCGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGc                                                                                                                                                                 <  1:256449/251‑1 (MQ=255)                                                                                       aTGTGAGCGTTACCAGGTTGTGTTGTGGCAGCGCCTGGTTGGTTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAg                                                                                                                                                       <  1:99141/251‑1 (MQ=255)                                                                                              cGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTg                                                                                                                                                 >  2:230095/1‑250 (MQ=255)                                                                                                             atcgggCAGCGCCAGGTTGGCTTCTAATTCCTGGCGTTTGGGATCTTCTAACATGTTGACTCCTTCGTGCCGGAGGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCGGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCtt                                                                                                                                 <  2:15113/247‑1 (MQ=255)                                                                                                                 ggCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCtt                                                                                                                                 >  1:15113/1‑247 (MQ=255)                                                                                                                  gCAGCGCCAGGTTGGCTTCTAATTCCTGGCGTTTGAGATCTTCTAGCATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCtt                                                                                                                            <  1:195600/251‑1 (MQ=255)                                                                                                                       gcCATGTTGGCTTCTAATTCCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGTAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCg                                                                                                                       <  1:44297/251‑1 (MQ=255)                                                                                                                                  ttCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAt                                                                                                            >  1:94090/1‑251 (MQ=255)                                                                                                                                  ttCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTAGTGCCGGATGCGCTTTGCTTATCAGGCCTACAAAATCGCAGCGTGTAGGCCTGATCAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGACCTCCAAAAt                                                                                                            >  2:25012/1‑251 (MQ=255)                                                                                                                                                      gaTCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGCTGCGATTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGCTAAGACGCACCAGCGTCGCATCAGGCGTTGCATGCCGAATGCGCTTTGCTTATCCAGCCTCCAAAATCGCAGCGTGTAGGcgagct                                                                                         >  2:5344/1‑245 (MQ=18)                                                                                                                                                                    ttGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGt                                                                          >  1:108957/1‑251 (MQ=255)                                                                                                                                                                               gTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCg                                                               >  1:209172/1‑251 (MQ=255)                                                                                                                                                                                         cgcTGTGCTTATCCGGACTACAAAATCGCAGCTTGTAGGCCTGATAATACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCTCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTTAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCATCGTCGCATCATGCGTTACATACCg                                                     <  2:174543/251‑1 (MQ=21)                                                                                                                                                                                              tGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGc                                                >  1:246597/1‑251 (MQ=255)                                                                                                                                                                                                   aTCCGGCCTACAAAACCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGTATCAGGCATTGAATGCCGGATGCGCTTTGCGTATCCGGCCTACAAAAGCGCAGCGCGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTGAAGGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTa                                           <  1:30897/251‑1 (MQ=21)                                                                                                                                                                                                      cGGCCTACAAAATCGCAGCGTGTAGGCCTGATCAGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACtt                                        >  1:216534/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaGACGCGCCAGCGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCcag        <  1:18166/251‑1 (MQ=255)                                                                                                                                                                                                                                            cgcCAGGGTCGTATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCcagcgcagc  <  2:7671/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCAGCGCAGC  >  NC_000913/66389‑66874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑