Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	47,434	T→C	H63H (CAT→CAC)	kefF →	regulator of KefC‑mediated potassium transport and quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	47,434	0	T	C	100.0%	57.7 / NA	22	H63H (CAT→CAC)	kefF	regulator of KefC‑mediated potassium transport and quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

GTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTG‑CTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTG  >  NC_000913/47190‑47675                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   gTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATg                                                                                                                                                                                                                                              <  1:9893/251‑1 (MQ=255)              tACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGCAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGc                                                                                                                                                                                                                                  >  1:40104/1‑250 (MQ=255)                          gAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGGTGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCGCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGctcc                                                                                                                                                                                                                     <  2:28810/251‑1 (MQ=255)                          gAAAACAAAGGCGTAATCACGCGGGCGACCTATGATTCTTATAATTTATGAGCATCCGTATCCGCATCAGTCCCATGCGAATAAACGGATGCTTTAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCTATCTGATCTTCTGGCAGCACCCGATTCATTGGTTCAGTATTCCTCTGctcc                                                                                                                                                                                                                     <  2:157699/251‑1 (MQ=255)                                    gCGTAATCACGCGGGCGTCCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAAGAAACGGCTGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTg                                                                                                                                                                                                           <  1:97061/251‑1 (MQ=255)                                                 ggCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGtt                                                                                                                                                                                              >  1:121224/1‑251 (MQ=255)                                                                                                        gAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGACGCTGCATGGCAACCAtt                                                                                                                                       >  2:9651/1‑251 (MQ=255)                                                                                                                        gAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGTGCGGTgac                                                                                                                       <  2:197277/251‑1 (MQ=255)                                                                                                                                     cGCTGGAAGGCGTCGAAATTCGCTCTCTTTCTCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTACGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCggg                                                                                                          >  1:237439/1‑251 (MQ=255)                                                                                                                                                  cGAAATGCGTTCTCTGTATCAACTCTAGCCTTGCCTCAATATCGGTATAACGGCCGGGGGGGAAGCCCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAtttt                                                                                              <  1:167389/250‑1 (MQ=255)                                                                                                                                                        tCGCTCTCTTTATCAACTCTCTCCTGACTTCACTATCGATATTGCCGCCGAGCACGAGGCACTGTCTCACGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTACGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGCGCTTACGGTCATGGCGGCCCGGCGCTGCATGGCAAACATTTGATGTGGGCGGTGACGACCGGCGGCGGGGGAAGCCATTTTGCAATTg                                                                                       >  2:120555/1‑251 (MQ=255)                                                                                                                                                         cGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTgg                                                                                      >  1:140446/1‑251 (MQ=255)                                                                                                                                                         cGCTCTCTTTATCAACTCTAGCCTGACTTCAATAGCGATATGGACGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACGTTGGATCGATAAAGTTTTCGCCCACGGCTGGGCTTTCGGTCATGGCGGCACGGCGCTGCATGGCAAACGTTTTATGTGGGCGGTGACGTCCGGCGGCGGGGAAAGCCagttgcaaattg                                                                                       <  2:39320/250‑12 (MQ=255)                                                                                                                                                           ctctctTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCAGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGACTGGCAGCACCCGATGCAGTGGGACAGCATTCCTACGCTCCTCAAACTTTGGATCGATAAAGTATTCTCCCACGGCTGGGCTTACGGTAATGGCGGCACGGCGCTGCAGGGAAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTgggg                                                                                    >  1:135211/1‑249 (MQ=255)                                                                                                                                                                        ctctATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAACCATTTTGAAATTGGTGCGCATCCGGGCtt                                                                       >  2:234520/1‑251 (MQ=255)                                                                                                                                                                                     tCAATATCGATATTGCCGCCGAGCAGGAGGCGCGGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCCCCCACGGCTGGGCTTACGGTCATGGCTGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTG‑CTgcc                                                          <  2:101256/250‑3 (MQ=255)                                                                                                                                                                                      cactatCGATATTGCCGCCGAGCAAGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGGAGGCCATTTTGTCATTGGGGCGCATCCGGGCTTTGATTTG‑CTggctc                                                        >  2:20392/4‑247 (MQ=255)                                                                                                                                                                                                        cgAGCCGGAGGCGATGTCTCGCGCCGATCTGATAGTCAGGCAGCCCCCGATGAAGTGGTACAGCATTCCTCCGCTCATCAACCTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGCCGGCACGGCGCTGCATGGCCAACATTTGCTGTGGGCGGGGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCACCCGGGCTTT‑ATTTGGCTGTCGCAGCAGCTACCGGCGaagg                                      >  2:161079/1‑248 (MQ=255)                                                                                                                                                                                                                    gcTGGCTCGCGCCGATCTGATCGTCTGGCGGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATATAGTTTGCTCCCGCGGCTGGGCTTACGGTCATGGCGGCTCGGCGCTGCATGGCAAACATGTGCGGTGGGCGGTGACGACCGGCGTCGGTGAAAGCGATTTTGAAATTGGTGCGCGTCCGGGCTGTGATGGG‑CTGTCGCGGCCTCTACTGGCGACGGCAATCTACTGCg                          <  2:259856/251‑1 (MQ=255)                                                                                                                                                                                                                        tctcGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGGGCGCATCCGGGCTTTGATGTG‑CTGTCGCAGCCGCTACAGGCGCCGGCAATCTACTGCGGGCt                      >  1:144688/1‑251 (MQ=255)                                                                                                                                                                                                                                      gATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTGGAAATGGGTGCGCATCCGGGCTTTGATGTG‑CTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTgg               <  2:121224/244‑1 (MQ=255)                                                                                                                                                                                                                                            cTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTG‑CTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGtttt  >  1:189620/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   GTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTG‑CTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTG  >  NC_000913/47190‑47675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑