Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	47,099	G→A	Q431Q (CAG→CAA)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	47,099	0	G	A	94.7%	47.7 / NA	19	Q431Q (CAG→CAA)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (10/8);  minor base C (1/0);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAGACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACA  >  NC_000913/46887‑47313                                                                                                                                                                                                                     |                                                                                                                                                                                                                       gAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGt                                                                                                                                                                                   >  1:211104/1‑250 (MQ=255)             aCAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGGCCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGAt                                                                                                                                                                      <  1:210059/251‑1 (MQ=255)                gATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGCTTTCCGTAGCGTTTGCCCCGGAGTCTCGAGGGATGTCACTGGCGCACACCAGCAATATGACGATCCGCGTGCAGCGAATGGGGTAAATTGTTCAGCTTtc                                                                                                                                                                   >  1:39320/1‑251 (MQ=255)                   atCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTtctct                                                                                                                                                                <  1:121719/251‑1 (MQ=255)                      cgcgCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCtt                                                                                                                                                             <  1:27564/251‑1 (MQ=255)                                ccGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGCGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATc                                                                                                                                                    >  1:170565/1‑250 (MQ=255)                                 cGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCCCTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGCGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCa                                                                                                                                                   >  2:222133/1‑250 (MQ=255)                                  gTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCCGTAACACGATGCTACTGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGTGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATccat                                                                                                                                                 >  1:259856/1‑248 (MQ=255)                                                               tGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAatata                                                                                                                     <  1:88402/250‑1 (MQ=255)                                                                        tGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCCGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACt                                                                                                           >  2:58748/1‑251 (MQ=255)                                                                          tttCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAAAACGATGCTAATGGGGGCGGGCATCTCGCTGTTTGGATTGATGATTTCCGTAGCGTTTACCCCGGAGACCCGAGGGAAGTCACTGGAGCAAACCAGCAATATGACGATACGCGGGCAGAGAATGGGGTGAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATCAGCCGCGTGAATATATTACTACACttt                                                                                                         >  1:113451/1‑251 (MQ=255)                                                                                                        ataatTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGc                                                                           <  1:73356/251‑1 (MQ=255)                                                                                                                   attagttgcacgttgttAATGGGGGCGGGTGTATCGCGGTTTGGCTTGTTGATTACCGTAGCGTTTGCCTCGGAGACTCGAGGGATGTCACTGGCGCAAACCATAAAGATGACGATCCTCGGGCAGAGAATGGGGTAAATTGTTCAGATGTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCTTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTaggag                                                                 <  2:6002/234‑5 (MQ=255)                                                                                                                                 gCTAATGGGGGCGGGTATCTCGCTGATTGGCTTGTTGATTTCCCTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGCAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCCCGCGGGCTACCTATGCTTCTTATAATTTCTgc                                                  >  2:97061/1‑251 (MQ=255)                                                                                                                                              ggTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCACTATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCg                                     >  1:197277/1‑251 (MQ=255)                                                                                                                                                    tcGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGcatcat                               >  1:28810/1‑251 (MQ=255)                                                                                                                                                                 tgttgATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAAt                   >  1:265743/1‑250 (MQ=255)                                                                                                                                                                  gttgATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATa                  <  1:222133/250‑1 (MQ=255)                                                                                                                                                                                 gTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGTGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACa  <  1:130699/251‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                       GAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAGACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACA  >  NC_000913/46887‑47313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑