Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,976	A→G	L390L (CTA→CTG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,976	0	A	G	100.0%	54.2 / NA	19	L390L (CTA→CTG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/9);  total (10/9)

GTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAGACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTT  >  NC_000913/46739‑47210                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           gtttaTTGGCAGCTTTGCCATGATGACGCTGGTGCGGGCGGTTTTGGGTCTGACCCCGGATTTGGGGATCGTGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGGGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTTGGCGTGATTATCTCCTTAAGCCGTATTGGCACCAATGTTTCGACCTGGGCACTGCAGATCTTTATCa                                                                                                                                                                                                                               <  2:165958/247‑1 (MQ=255)                      gatgaCGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAaca                                                                                                                                                                                                          <  1:133136/251‑1 (MQ=255)                                    tggcgGTTTTGGGGCTGATACCGGTTATGGGGATCTGGCTGGGAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTTCAGTGGCTCTATCCTTATGAACTCTTCCCGACAGATATCCGCGCCTCTTCCCTGGGCGTGATTATTTCCTTAAGCCGTATTGGCCCCCTTGTTTCGTCCTGGGCCCTGCCCGTCTTTTTCAATTATTACGGTTTCAGTAACCCGATGCTAAtcgtg                                                                                                                                                                                            >  2:9893/1‑247 (MQ=255)                                        ggTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATg                                                                                                                                                                                               <  2:2754/244‑1 (MQ=255)                                        ggTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGCTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATg                                                                                                                                                                                               >  1:2754/1‑244 (MQ=255)                                                       cccGGATGTGGGGAGCTGGCTGGTAGTGTTGGCATTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGGGGCTCTATCCTAATGAACTCTTCCCTACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGATCTGGGCACTGCCGATCTTTATCAATAATTACGGTAACAGTTTCACGATGCTAAGGGGGGCTAGTATCTCGCTGttt                                                                                                                                                                          <  2:174278/250‑1 (MQ=255)                                                          ggATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCt                                                                                                                                                                      <  1:60832/251‑1 (MQ=255)                                                                                        cTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGATCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCgg                                                                                                                                           >  2:161915/1‑248 (MQ=255)                                                                                                                                                     gAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGt                                                                            >  1:211104/1‑250 (MQ=255)                                                                                                                                                                 aCAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGGCCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGAt                                                               <  1:210059/251‑1 (MQ=255)                                                                                                                                                                    gATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGCTTTCCGTAGCGTTTGCCCCGGAGTCTCGAGGGATGTCACTGGCGCACACCAGCAATATGACGATCCGCGTGCAGCGAATGGGGTAAATTGTTCAGCTTtc                                                            >  1:39320/1‑251 (MQ=255)                                                                                                                                                                       atCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTtctct                                                         <  1:121719/251‑1 (MQ=255)                                                                                                                                                                          cgcgCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCtt                                                      <  1:27564/251‑1 (MQ=255)                                                                                                                                                                                    ccGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGCGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATc                                             >  1:170565/1‑250 (MQ=255)                                                                                                                                                                                     cGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCCCTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGCGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCa                                            >  2:222133/1‑250 (MQ=255)                                                                                                                                                                                      gTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCCGTAACACGATGCTACTGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGTGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATccat                                          >  1:259856/1‑248 (MQ=255)                                                                                                                                                                                                                   tGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAatata              <  1:88402/250‑1 (MQ=255)                                                                                                                                                                                                                            tGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCCGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACt    >  2:58748/1‑251 (MQ=255)                                                                                                                                                                                                                              tttCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAAAACGATGCTAATGGGGGCGGGCATCTCGCTGTTTGGATTGATGATTTCCGTAGCGTTTACCCCGGAGACCCGAGGGAAGTCACTGGAGCAAACCAGCAATATGACGATACGCGGGCAGAGAATGGGGTGAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATCAGCCGCGTGAATATATTACTACACttt  >  1:113451/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           GTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAGACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTT  >  NC_000913/46739‑47210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑