Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,790	A→G	L328L (CTA→CTG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,790	0	A	G	100.0%	55.1 / NA	23	L328L (CTA→CTG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

CCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGG  >  NC_000913/46555‑47024                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            ccATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTAGGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCTGACGGCGTCCATGGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATgggg                                                                                                                                                                                                                              <  1:264867/251‑1 (MQ=255)    tCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGGGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGCTTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGGTTTTGGGCCGATCCCGGCTCTgggggtt                                                                                                                                                                                                                           >  2:121719/1‑248 (MQ=255)            tGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGGTTTGGGGCTGAACCCGGATATGGGGATttggggtgg                                                                                                                                                                                                                   >  2:60832/1‑246 (MQ=255)                aGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATGGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGGTCTTTATGCTAGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCGGGCTGTTAGTg                                                                                                                                                                                                               <  2:37492/251‑1 (MQ=255)                 ggTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGCTGCTTTGATGGGCTTGGGTGTTGGAAAACACGCGGCGCTGGGGAACGTGGTGATTAGCATGTACTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACTGACTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGCTTTGGGGCTGTTCCCgggattcgggaactagctgtgagca                                                                                                                                                                                                               >  1:264940/1‑227 (MQ=255)                             gTTCGCCATTTAAACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCACAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATACTGGGTATGGGGGTCTGGGTGGGAGTG‑AAGGCCTTTGCgg                                                                                                                                                                                                 >  2:88402/1‑251 (MQ=255)                                  ccATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAATCGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGAGCTTTCTGGTAGTG‑ATGGCCTTTGCGGTGTAt                                                                                                                                                                                            <  2:16473/251‑1 (MQ=255)                                  ccATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTAt                                                                                                                                                                                            <  1:26258/251‑1 (MQ=255)                                         cacCTTTGGCCCGCAAATCGTGGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACTTGGTGATTAGCCTTTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGTGATCTGGCTGGTAGTG‑ATGGCCTTAGCGGTGTAGGCCtgtt                                                                                                                                                                                     <  2:60237/251‑4 (MQ=255)                                                                gTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGTTGGGGAACGTGGTGATTAGCCTGTTCTTTAGGCTCGGCTGTATTCCTCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCTGTTTTGGGGCTGATCTCGGATATTGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGTCCGGGTAATTTg                                                                                                                                                              <  2:236166/251‑1 (MQ=255)                                                                              ggtgTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATcc                                                                                                                                                <  1:178739/248‑1 (MQ=255)                                                                                                          acgtggtgATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCcgc                                                                                                                    <  2:69318/249‑1 (MQ=255)                                                                                                                           ttCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCTGATCCCGGATTTGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTTTCTGGCGGGCCGGGGTATTTTCAGGGGGTCTTTCCTAATGAATTCTTCCCGCCAGctattcccgcccctcccgggggggg                                                                                                   >  2:73356/1‑230 (MQ=255)                                                                                                                                 aTGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTAt                                                                                             <  1:212073/251‑1 (MQ=255)                                                                                                                                             aTTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAgccg                                                                                 <  1:62472/251‑4 (MQ=255)                                                                                                                                                    cgATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATTCCGGGTATGGGGATCTGGCTGGGAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGGAATTTGCAGTGGGTCTTTCCTAATGAACTCTTTCCGGCAGAAATCCCCGCCTCTGCCGTGGGGGTGATTTTGTCCTTAAACCGTATTGGc                                                                          >  2:133136/1‑251 (MQ=255)                                                                                                                                                              gTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCGGATCCCGGATATGGGGATCTGGCTGGTAGGG‑ATGGCCTTTGTGGGGTATGCCTTTTTCTCTGGCGGGCCGGGGGATTTGCAGTGGCTCTTTCCTAATGTACTCTTCCCCACAGAGATCCGCGCCCCTGCCGTGGGCGTGGTTATGTCCTTAAACCGTATTGGCACCATTGttt                                                                >  2:210059/1‑251 (MQ=255)                                                                                                                                                                                  gTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTTGGGCTGATTCCGGATAGTGGGATCTGGCTGGGAGTGTATGGC‑TTTGCGGGGGTTGCCTTTTTCTCTGGGGGGGCGGGGGATTTGGCGTGGGTCTATACTTATGAACTCATCCCGACCGATATACGCGCCTCTGGCGGTGGCGGGATTGTGTCCTTAATGCGTATTGGCACCAtttttttggcctcggtacggcccatc                                            >  2:130699/1‑231 (MQ=255)                                                                                                                                                                                         gtttaTTGGCAGCTTTGCCATGATGACGCTGGTGCGGGCGGTTTTGGGTCTGACCCCGGATTTGGGGATCGTGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGGGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTTGGCGTGATTATCTCCTTAAGCCGTATTGGCACCAATGTTTCGACCTGGGCACTGCAGATCTTTATCa                                     <  2:165958/247‑1 (MQ=255)                                                                                                                                                                                                              gatgaCGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAaca                <  1:133136/251‑1 (MQ=255)                                                                                                                                                                                                                            tggcgGTTTTGGGGCTGATACCGGTTATGGGGATCTGGCTGGGAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTTCAGTGGCTCTATCCTTATGAACTCTTCCCGACAGATATCCGCGCCTCTTCCCTGGGCGTGATTATTTCCTTAAGCCGTATTGGCCCCCTTGTTTCGTCCTGGGCCCTGCCCGTCTTTTTCAATTATTACGGTTTCAGTAACCCGATGCTAAtcgtg  >  2:9893/1‑247 (MQ=255)                                                                                                                                                                                                                                ggTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATg     <  2:2754/244‑1 (MQ=255)                                                                                                                                                                                                                                ggTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGCTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATg     >  1:2754/1‑244 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            CCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGG  >  NC_000913/46555‑47024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑