Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,655	A→G	L283L (CTA→CTG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,655	0	A	G	100.0%	60.9 / NA	21	L283L (CTA→CTG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/13);  total (8/13)

CGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCC  >  NC_000913/46419‑46882                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    cGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGAt                                                                                                                                                                                                                       <  1:164797/251‑1 (MQ=255)     tAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGc                                                                                                                                                                                                                   >  1:37492/1‑251 (MQ=255)       aaaGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCt                                                                                                                                                                                                                 >  2:62472/1‑251 (MQ=255)                                                            ggAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGCTTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGAc                                                                                                                                                            >  2:27564/2‑251 (MQ=255)                                                                    agctgcagcaAACC‑GTTTGTCGCGTTCTGTTGAATCGCCGCCATTTTCCTTTTGTTCTGTTGGGTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTGTGTTGGGATTGGGTGGTGGCAAAATCGCGGCGCTGGGGATCGTGGTGATTAGTCTGGTCTGTATGCACGGCGGTATGCCGCCGATGTTCTGTTTAATCACTGCCGGACGGGGTCCa                                                                                                                                                    <  2:14092/247‑1 (MQ=255)                                                                          agcaAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCAttgttg                                                                                                                                              <  1:148703/251‑1 (MQ=255)                                                                                                 gTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCatg                                                                                                                            <  1:71783/247‑1 (MQ=255)                                                                                                          ccgccATTTTCCTTTTGTTCTGTTTTTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTGACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTccgc                                                                                                                                                                                       <  1:140317/179‑1 (MQ=255)                                                                                                          ccgccATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGCCCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTccgc                                                                                                                                                                                       >  2:140317/1‑179 (MQ=255)                                                                                                                            tCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACTTTGGGCCCGCAATTCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCtg                                                                                              <  2:227521/250‑2 (MQ=255)                                                                                                                                          ccATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTAGGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCTGACGGCGTCCATGGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATgggg                                                                               <  1:264867/251‑1 (MQ=255)                                                                                                                                             tCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGGGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGCTTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGGTTTTGGGCCGATCCCGGCTCTgggggtt                                                                            >  2:121719/1‑248 (MQ=255)                                                                                                                                                     tGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGGTTTGGGGCTGAACCCGGATATGGGGATttggggtgg                                                                    >  2:60832/1‑246 (MQ=255)                                                                                                                                                         aGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATGGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGGTCTTTATGCTAGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCGGGCTGTTAGTg                                                                <  2:37492/251‑1 (MQ=255)                                                                                                                                                          ggTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGCTGCTTTGATGGGCTTGGGTGTTGGAAAACACGCGGCGCTGGGGAACGTGGTGATTAGCATGTACTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACTGACTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGCTTTGGGGCTGTTCCCgggattcgggaactagctgtgagca                                                                >  1:264940/1‑227 (MQ=255)                                                                                                                                                                      gTTCGCCATTTAAACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCACAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATACTGGGTATGGGGGTCTGGGTGGGAGTGAAGGCCTTTGCgg                                                   >  2:88402/1‑251 (MQ=255)                                                                                                                                                                           ccATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAATCGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGAGCTTTCTGGTAGTGATGGCCTTTGCGGTGTAt                                              <  2:16473/251‑1 (MQ=255)                                                                                                                                                                           ccATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTAt                                              <  1:26258/251‑1 (MQ=255)                                                                                                                                                                                  cacCTTTGGCCCGCAAATCGTGGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACTTGGTGATTAGCCTTTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGTGATCTGGCTGGTAGTGATGGCCTTAGCGGTGTAGGCCtgtt                                       <  2:60237/251‑4 (MQ=255)                                                                                                                                                                                                         gTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGTTGGGGAACGTGGTGATTAGCCTGTTCTTTAGGCTCGGCTGTATTCCTCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCTGTTTTGGGGCTGATCTCGGATATTGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGTCCGGGTAATTTg                <  2:236166/251‑1 (MQ=255)                                                                                                                                                                                                                       ggtgTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATcc  <  1:178739/248‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    CGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCC  >  NC_000913/46419‑46882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑