Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,634	G→T	G276G (GGG→GGT)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,634	0	G	T	100.0%	56.5 / NA	21	G276G (GGG→GGT)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/12);  total (9/12)

ACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTG  >  NC_000913/46409‑46868                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           aCGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGACGCAGCAAACCCGTTT‑TAGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGcgctgggg                                                                                                                                                                                                                    >  1:44914/1‑243 (MQ=255)           cGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGAt                                                                                                                                                                                                         <  1:164797/251‑1 (MQ=255)               tAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGc                                                                                                                                                                                                     >  1:37492/1‑251 (MQ=255)                 aaaGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCt                                                                                                                                                                                                   >  2:62472/1‑251 (MQ=255)                                                                      ggAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGCTTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGAc                                                                                                                                              >  2:27564/2‑251 (MQ=255)                                                                              agctgcagcaAACC‑GTTTGTCGCGTTCTGTTGAATCGCCGCCATTTTCCTTTTGTTCTGTTGGGTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTGTGTTGGGATTGGGTGGTGGCAAAATCGCGGCGCTGGGGATCGTGGTGATTAGTCTGGTCTGTATGCACGGCGGTATGCCGCCGATGTTCTGTTTAATCACTGCCGGACGGGGTCCa                                                                                                                                      <  2:14092/247‑1 (MQ=255)                                                                                    agcaAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCAttgttg                                                                                                                                <  1:148703/251‑1 (MQ=255)                                                                                                           gTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCatg                                                                                                              <  1:71783/247‑1 (MQ=255)                                                                                                                    ccgccATTTTCCTTTTGTTCTGTTTTTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTGACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTccgc                                                                                                                                                                         <  1:140317/179‑1 (MQ=255)                                                                                                                    ccgccATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGCCCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTccgc                                                                                                                                                                         >  2:140317/1‑179 (MQ=255)                                                                                                                                      tCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACTTTGGGCCCGCAATTCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCtg                                                                                <  2:227521/250‑2 (MQ=255)                                                                                                                                                    ccATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTAGGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCTGACGGCGTCCATGGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATgggg                                                                 <  1:264867/251‑1 (MQ=255)                                                                                                                                                       tCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGGGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGCTTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGGTTTTGGGCCGATCCCGGCTCTgggggtt                                                              >  2:121719/1‑248 (MQ=255)                                                                                                                                                               tGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGGTTTGGGGCTGAACCCGGATATGGGGATttggggtgg                                                      >  2:60832/1‑246 (MQ=255)                                                                                                                                                                   aGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATGGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGGTCTTTATGCTAGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCGGGCTGTTAGTg                                                  <  2:37492/251‑1 (MQ=255)                                                                                                                                                                    ggTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGCTGCTTTGATGGGCTTGGGTGTTGGAAAACACGCGGCGCTGGGGAACGTGGTGATTAGCATGTACTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACTGACTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGCTTTGGGGCTGTTCCCgggattcgggaactagctgtgagca                                                  >  1:264940/1‑227 (MQ=255)                                                                                                                                                                                gTTCGCCATTTAAACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCACAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATACTGGGTATGGGGGTCTGGGTGGGAGTGAAGGCCTTTGCgg                                     >  2:88402/1‑251 (MQ=255)                                                                                                                                                                                     ccATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAATCGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGAGCTTTCTGGTAGTGATGGCCTTTGCGGTGTAt                                <  2:16473/251‑1 (MQ=255)                                                                                                                                                                                     ccATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTAt                                <  1:26258/251‑1 (MQ=255)                                                                                                                                                                                            cacCTTTGGCCCGCAAATCGTGGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACTTGGTGATTAGCCTTTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGTGATCTGGCTGGTAGTGATGGCCTTAGCGGTGTAGGCCtgtt                         <  2:60237/251‑4 (MQ=255)                                                                                                                                                                                                                   gTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGTTGGGGAACGTGGTGATTAGCCTGTTCTTTAGGCTCGGCTGTATTCCTCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCTGTTTTGGGGCTGATCTCGGATATTGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGTCCGGGTAATTTg  <  2:236166/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           ACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTT‑TCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTG  >  NC_000913/46409‑46868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑