Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,978	C→T	L58L (CTG→TTG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,978	0	C	T	100.0%	88.5 / NA	30	L58L (CTG→TTG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

TTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCC  >  NC_000913/45741‑46218                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 ttACGGCTGAAGGTGGTTTGATACGTAACGCCGCACTGCTTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGCGCAAGTGACGCCGGCGCTAACACTGGACGCTGACTGGCTTGGCTTGTTGGGCGCGGGAAc                                                                                                                                                                                                                                     >  2:101166/1‑251 (MQ=255)                  tggatccgaaaccccgagcTGACTGTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAACCTTGGGCGTTCTCAAATTCCCCTCTATCCACCGCCGCATTTTGCGGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACTCGGACTGGAGTGGCGTGTTGGGCGCGTGAACGCTCGCCGGGCTGTTc                                                                                                                                                                                                                     <  2:131418/232‑1 (MQ=255)                    gATACGTAACGACGCACTGACTCTCATTGAAAAAAACAGGAATAACCATGAAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCGCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGtt                                                                                                                                                                                                                  <  1:9535/251‑1 (MQ=255)                     aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTg                                                                                                                                                                                                                 >  1:61882/1‑251 (MQ=255)                                          ctcATTGCAAAAAAAAGGCATCACCATGCAACCGTCCAGAACCTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGTTTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCtat                                                                                                                                                                                             >  2:180865/1‑250 (MQ=255)                                                   aaaaaCAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGATGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGAt                                                                                                                                                                                    >  1:132432/1‑250 (MQ=255)                                                    aaaaCAGGAATAACCATCCACCCGTCCAGAAACTTTGTCGATCTCAAATTCTCCTCTATCCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCCCATCGCTGTTTGGCTATATTTCCGATaa                                                                                                                                                                                  <  2:55811/251‑1 (MQ=255)                                                    aaaaCAGGAATAACCATCCAACCGTCCAGAACTTTTGACGATCTCAAATTCGCCTCTATCCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACCCCGGCGCTAAAACTGGACTCTGACCGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCAGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATaa                                                                                                                                                                                  <  2:29674/251‑1 (MQ=255)                                                      aaCAGGAATACCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTCCTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTACAACTGGACGCTGACTGGCTTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATaa                                                                                                                                                                                  >  1:180698/1‑249 (MQ=255)                                                       aCAGGAATAACCATGCAACCGTCCAGAAACTTTGAGGATCTCAACTTCTCCTCTATTCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTGTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGGCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGt                                                                                                                                                                               <  2:21928/251‑1 (MQ=255)                                                            aaTAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGAc                                                                                                                                                                          >  1:136897/1‑251 (MQ=255)                                                                   aTGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCa                                                                                                                                                                    >  1:28787/1‑250 (MQ=255)                                                                     gCAACCGTCCGAAAACTTGGACGATTCCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGGCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCGGGAGCAACGGACGCCGTCGCTAAAACTGGACGCTGGCTGGATTGGCTGGTTTGGCGCGGGAACGCTCTCCGGGCTGTTCGTTGGCACATCGCTGTGTGGCTATATTTCCGATAAAGTCGGTCGGCGCaaaa                                                                                                                                                                 <  2:180698/251‑1 (MQ=255)                                                                                                   aTTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCGGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCg                                                                                                                                   <  1:247634/251‑1 (MQ=255)                                                                                                        cctcTATCCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAAGGGTCCTCATTGATATCATCGCCATCGGCGTg                                                                                                                                 <  1:209144/248‑1 (MQ=255)                                                                                                          tctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATc                                                                                                                            >  2:196371/1‑251 (MQ=255)                                                                                                                               cTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTc                                                                                                       >  1:2632/1‑251 (MQ=255)                                                                                                                                                                 aTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTGGTTGGGCGCGGGAACGCTCGCCGGGCTGTGCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCGTCGGCGTGATAGCGGGGGGGACGATGGTTGTTTCAGCCCCCGGCGAACTGTTGGTGATTCGGGTACtt                                                                     <  2:132432/251‑1 (MQ=255)                                                                                                                                                                   gTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTa                                                                    <  2:129380/250‑1 (MQ=255)                                                                                                                                                                     aCTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCg                                                                 <  1:8828/251‑1 (MQ=255)                                                                                                                                                                            aTGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGt                                                          <  2:248914/251‑1 (MQ=255)                                                                                                                                                                                          gcTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTAGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTTTCATCGGGGCatgtt                                            >  2:144968/1‑247 (MQ=255)                                                                                                                                                                                              gAGCAACTGTCGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACAGCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCAGTCGAACTGTTGGTGATGCTGGGACTTATCGGCATTGTCATCGGTGCAGATTATcc                                        <  2:193631/251‑1 (MQ=255)                                                                                                                                                                                                 gaaCTGACGGCGGCGCTAAAACTGGACGCTGACTGGATTGGCGTGTTGGGCGCGGGAACGCGCGTCGGGCTGGTCTGTGGCACATCGCTGTTTGGCGATATGTCCGATAAGGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGTCATTGTCATCGGTGCAGATTATCCCAt                                     <  1:258919/250‑1 (MQ=255)                                                                                                                                                                                                              cgcTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATg                        >  1:34121/1‑251 (MQ=255)                                                                                                                                                                                                                    aaCTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCAcc                  <  1:150582/251‑1 (MQ=255)                                                                                                                                                                                                                          aCGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTtc            >  1:237522/1‑251 (MQ=255)                                                                                                                                                                                                                              tGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTcc         >  1:206461/1‑250 (MQ=255)                                                                                                                                                                                                                               gACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCTGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTTCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTTCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAg       <  1:105442/251‑1 (MQ=255)                                                                                                                                                                                                                                    gATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGCCGGCGCAAACTGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGAGACGCTGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTAccc  >  1:14092/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 TTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCC  >  NC_000913/45741‑46218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑