Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,950	G→A	L48L (CTG→CTA)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,950	0	G	A	100.0%	81.9 / NA	28	L48L (CTG→CTA)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)

GCGCGGCACCTTTGGTGTGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATG  >  NC_000913/45714‑46196                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       gcgcgGCACCTTTGGTGTGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGAACTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAGGCGGGGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGGGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTgg                                                                                                                                                                                                                                          >  2:130658/1‑250 (MQ=255)   gcgGCACCTTTGGTGTGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACt                                                                                                                                                                                                                                        <  1:81558/251‑1 (MQ=255)     ggCACCTTTGGAGGGGAGTTCCGTTACGGCAGATGTTGGTTTGCTACGTAACGCCGCACTGAATCTCAGTGAAAAAAACAGGAATAACCATGCAAACGTCCAGACACTTTGATGATCTCAAATTCTCCTCTCTTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTgg                                                                                                                                                                                                                                      >  2:105442/1‑251 (MQ=255)                            ttACGGCTGAAGGTGGTTTGATACGTAACGCCGCACTGCTTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGCGCAAGTGACGCCGGCGCTAACACTGGACGCTGACTGGCTTGGCTTGTTGGGCGCGGGAAc                                                                                                                                                                                                               >  2:101166/1‑251 (MQ=255)                                             tggatccgaaaccccgagcTGACTGTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAACCTTGGGCGTTCTCAAATTCCCCTCTATCCACCGCCGCATTTTGCGGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACTCGGACTGGAGTGGCGTGTTGGGCGCGTGAACGCTCGCCGGGCTGTTc                                                                                                                                                                                               <  2:131418/232‑1 (MQ=255)                                               gATACGTAACGACGCACTGACTCTCATTGAAAAAAACAGGAATAACCATGAAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCGCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGtt                                                                                                                                                                                            <  1:9535/251‑1 (MQ=255)                                                aTACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTg                                                                                                                                                                                           >  1:61882/1‑251 (MQ=255)                                                                     ctcATTGCAAAAAAAAGGCATCACCATGCAACCGTCCAGAACCTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGTTTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCtat                                                                                                                                                                       >  2:180865/1‑250 (MQ=255)                                                                              aaaaaCAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGATGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGAt                                                                                                                                                              >  1:132432/1‑250 (MQ=255)                                                                               aaaaCAGGAATAACCATCCACCCGTCCAGAAACTTTGTCGATCTCAAATTCTCCTCTATCCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCCCATCGCTGTTTGGCTATATTTCCGATaa                                                                                                                                                            <  2:55811/251‑1 (MQ=255)                                                                               aaaaCAGGAATAACCATCCAACCGTCCAGAACTTTTGACGATCTCAAATTCGCCTCTATCCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACCCCGGCGCTAAAACTGGACTCTGACCGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCAGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATaa                                                                                                                                                            <  2:29674/251‑1 (MQ=255)                                                                                 aaCAGGAATACCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTCCTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTACAACTGGACGCTGACTGGCTTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATaa                                                                                                                                                            >  1:180698/1‑249 (MQ=255)                                                                                  aCAGGAATAACCATGCAACCGTCCAGAAACTTTGAGGATCTCAACTTCTCCTCTATTCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTGTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGGCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGt                                                                                                                                                         <  2:21928/251‑1 (MQ=255)                                                                                       aaTAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGAc                                                                                                                                                    >  1:136897/1‑251 (MQ=255)                                                                                              aTGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCa                                                                                                                                              >  1:28787/1‑250 (MQ=255)                                                                                                gCAACCGTCCGAAAACTTGGACGATTCCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGGCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCGGGAGCAACGGACGCCGTCGCTAAAACTGGACGCTGGCTGGATTGGCTGGTTTGGCGCGGGAACGCTCTCCGGGCTGTTCGTTGGCACATCGCTGTGTGGCTATATTTCCGATAAAGTCGGTCGGCGCaaaa                                                                                                                                           <  2:180698/251‑1 (MQ=255)                                                                                                                              aTTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCGGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCg                                                                                                             <  1:247634/251‑1 (MQ=255)                                                                                                                                   cctcTATCCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAAGGGTCCTCATTGATATCATCGCCATCGGCGTg                                                                                                           <  1:209144/248‑1 (MQ=255)                                                                                                                                     tctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATc                                                                                                      >  2:196371/1‑251 (MQ=255)                                                                                                                                                          cTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTc                                                                                 >  1:2632/1‑251 (MQ=255)                                                                                                                                                                                            aTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTGGTTGGGCGCGGGAACGCTCGCCGGGCTGTGCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCGTCGGCGTGATAGCGGGGGGGACGATGGTTGTTTCAGCCCCCGGCGAACTGTTGGTGATTCGGGTACtt                                               <  2:132432/251‑1 (MQ=255)                                                                                                                                                                                              gTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTa                                              <  2:129380/250‑1 (MQ=255)                                                                                                                                                                                                aCTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCg                                           <  1:8828/251‑1 (MQ=255)                                                                                                                                                                                                       aTGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGt                                    <  2:248914/251‑1 (MQ=255)                                                                                                                                                                                                                     gcTGGAGCAACTGACGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTAGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTTTCATCGGGGCatgtt                      >  2:144968/1‑247 (MQ=255)                                                                                                                                                                                                                         gAGCAACTGTCGCCGGCGCTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACAGCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCAGTCGAACTGTTGGTGATGCTGGGACTTATCGGCATTGTCATCGGTGCAGATTATcc                  <  2:193631/251‑1 (MQ=255)                                                                                                                                                                                                                            gaaCTGACGGCGGCGCTAAAACTGGACGCTGACTGGATTGGCGTGTTGGGCGCGGGAACGCGCGTCGGGCTGGTCTGTGGCACATCGCTGTTTGGCGATATGTCCGATAAGGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGTCATTGTCATCGGTGCAGATTATCCCAt               <  1:258919/250‑1 (MQ=255)                                                                                                                                                                                                                                         cgcTAAAACTGGACGCTGACTGGATTGGCTTGTTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATg  >  1:34121/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       GCGCGGCACCTTTGGTGTGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATG  >  NC_000913/45714‑46196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑