Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	27,026	C→T	D250D (GAC→GAT)	ispH →	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	27,026	0	C	T	94.9%	109.1 / NA	39	D250D (GAC→GAT)	ispH	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/22);  minor base G (0/2);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTAC  >  NC_000913/26779‑27270                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     cGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATc                                                                                                                                                                                                                                                   <  2:54192/251‑1 (MQ=255)    tGTCGGTGGATGACAAGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCAGGCCCAGCGTATGGGCAACCGCGCGTTTTTGATTGACGATGCGAAAGATATcc                                                                                                                                                                                                                                                  >  1:92740/1‑249 (MQ=255)        ggTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAag                                                                                                                                                                                                                                            <  1:107199/251‑1 (MQ=255)            gATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGAGATCCAGGAAGAGTg                                                                                                                                                                                                                                        <  1:66492/251‑1 (MQ=255)                        gATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAAGTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGAGTGACGATGCGAAAGATATCCAGGAAGAGTGTGTGAAagag                                                                                                                                                                                                                              <  1:266270/249‑1 (MQ=255)                                           gTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACt                                                                                                                                                                                                         <  1:55454/251‑1 (MQ=255)                                           gTAAACGCTACCCGAACCTTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAACCGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGGAGGGTGGGTGACAGAGGTTCAATGCGTCGGCGTGAcc                                                                                                                                                                                                         >  2:119648/1‑250 (MQ=255)                                             aaaCGCTTCCCGAAAATTGGTGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAGCCGTCAGGAAGCTGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGTTTCGAAAAACTCCGCCAACTCCAACCGGCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATTTCCAGGAAGAGTGGGTGAAAGAGGTTAACTGCGTCGGCGTGTCTGc                                                                                                                                                                                                       <  2:106695/251‑1 (MQ=255)                                                     cccGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCACTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGTTGAAAGAGGGTAAATGCGTCGGCGTGACTGCGGGCGCAt                                                                                                                                                                                               <  2:92001/251‑1 (MQ=255)                                                      ccGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATc                                                                                                                                                                                              >  1:183509/1‑251 (MQ=255)                                                              tGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGCAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCgg                                                                                                                                                                                      >  1:111750/1‑251 (MQ=255)                                                                    tCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGGGGGTGAAAGAGGGTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTc                                                                                                                                                                                >  1:9402/1‑251 (MQ=255)                                                                           aaaGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCa                                                                                                                                                                         <  1:17643/251‑1 (MQ=255)                                                                                  aCATCTGCTACGCCACTACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGGGCTGGCCCAGCGTATGGACAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTTGGTTCACGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCCGATATTCTGGTGCaaactctg                                                                                                                                                                  >  1:97447/1‑244 (MQ=255)                                                                                   cATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATgtgg                                                                                                                                                                 <  1:232596/251‑1 (MQ=255)                                                                                        gCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGtggtgg                                                                                                                                                              <  1:244727/249‑1 (MQ=255)                                                                                              ccACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTg                                                                                                                                                      >  1:110215/1‑251 (MQ=255)                                                                                                          gTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGTAGCTGGGc                                                                                                                                          <  1:95101/251‑1 (MQ=255)                                                                                                            cAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCgg                                                                                                                                        <  1:90892/251‑1 (MQ=255)                                                                                                                                ggCAGAACAGGGGGAAGTTGTGTGGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGGGAAAGAGGTTAAATGCGGCGGCGTGACTGCGGGCGCATCGGGGCCGGATGTTCTGGTGCAAAATGGGGTGGCACGTTTTCAGCAGCGTGGCGGTGGTCAAGCCATTCTGCTgg                                                                                                                    <  2:45615/251‑1 (MQ=255)                                                                                                                                ggCAGAACAGGCGGAAGTTGGGTTGGTGGTCGGTTCGAAAAACTCCGCCAACTCCAACCGTCTTGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCAGTCCGCTg                                                                                                                     <  2:171717/250‑1 (MQ=255)                                                                                                                                                 ttGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCTCTGGAAGGCCGTGAAGaaacc                                                                                                   >  2:81488/1‑249 (MQ=255)                                                                                                                                                  tgtgTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAAc                                                                                                   <  1:207589/250‑1 (MQ=255)                                                                                                                                                     gtTGGAGGGCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGAGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGAGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGCAGAAAACATTg                                                                                               >  2:98131/1‑251 (MQ=255)                                                                                                                                                           ggtCGGTTCGAAAAACTCCGCCAACGCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCg                                                                                         <  2:183509/251‑1 (MQ=255)                                                                                                                                                               ggTTCGAAAACCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGAGAATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGCGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGCAGAAAACATTGTTTTCGTAGt                                                                                     >  2:144143/1‑251 (MQ=255)                                                                                                                                                                                               ggCGGAGCTGGCCCAGCGTAGGGGCAAACGCGCGTTTTTGATTGACGATGCGAGAGATAGCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCTGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGGGGCTCGTTTGCAGAAGCTGGGCGGTTGTGAAGCCAGTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGa                                                       <  2:264985/249‑1 (MQ=255)                                                                                                                                                                                               ggCGGAGATGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGTCGATGCGAAAGATAGCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGTCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCTGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAATCATGGTTTTCGAATTGCCGAAAGAGCTGCGTGTCGATATTCGTGa                                                       <  2:182533/249‑1 (MQ=255)                                                                                                                                                                                                    aGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGAATGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTTGTGCAAAATGTGTTGGCACGTTTGCAGCAGCTGTGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGAt                                                <  1:119648/251‑1 (MQ=255)                                                                                                                                                                                                    aGCTGGCACAGGGTATGGGCAAAAGCGGGTTGTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAAGGCCTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGATCAAAAGGAGGTGGCACGTTTGCAGTAGCTGGTCGGTGGTGAATCCATACCGCTGTAATGCCTTGAAGAAAACATTGTTTTCTAAGTGCCTAAAGAGCTGCGTATCGATATTCGTGAAGTCGAt                                                <  1:259426/251‑1 (MQ=255)                                                                                                                                                                                                                  aTGGGCAAACGCGCGTGTTTGATTGACGAGGCGAAAGAGATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGGCGGCGTGACTGCGGGCGCATCGGCGCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGGCGATTAAGTCATTagcag                                  <  2:168010/251‑1 (MQ=255)                                                                                                                                                                                                                   tGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagcagc                                 >  1:44292/1‑251 (MQ=255)                                                                                                                                                                                                                        aaaCGCACGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGCTATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGGGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGGGCCGAAAGAGCTGCGTGTCGATATTCGTGACGTCGATTAAGTCATTAGCAGCCTacg                            >  2:247637/1‑249 (MQ=255)                                                                                                                                                                                                                         aaCGCGCGTTTTTGATTGACGAAGCGGAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTACGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTAAAGAAAACATAGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCGAAGt                           >  1:176266/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttgattgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCt                                                                         >  1:148018/1‑194 (MQ=255)                                                                                                                                                                                                                                    ttgattgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCt                                                                         <  2:148018/194‑1 (MQ=255)                                                                                                                                                                                                                                       agtgaCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTGCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGcc             <  1:128067/249‑1 (MQ=255)                                                                                                                                                                                                                                             gATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATAGTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCtt       <  2:110215/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTAc  >  2:67416/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     CGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTAC  >  NC_000913/26779‑27270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑