Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	26,663	G→T	G129G (GGG→GGT)	ispH →	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	26,663	0	G	T	96.9%	69.3 / NA	33	G129G (GGG→GGT)	ispH	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (11/20);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCT  >  NC_000913/26420‑26905                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   cTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATACGGCAGTGGAAGGTACGATgg                                                                                                                                                                                                                                             >  1:215648/1‑251 (MQ=255)  ttGCGTGAGCGTGTGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGCCGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCAGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGGACGATggg                                                                                                                                                                                                                                            >  1:269527/1‑251 (MQ=255)  ttGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTACTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGAGGAAGGTACGATggg                                                                                                                                                                                                                                            >  1:264985/1‑251 (MQ=255)   tGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGGAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATGCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATggg                                                                                                                                                                                                                                            <  1:122352/250‑1 (MQ=255)                ggCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAAcc                                                                                                                                                                                                                              <  1:50281/251‑1 (MQ=255)                              aGCAGATTAGCGAAGTACCGGAGGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAACGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGTAGGTCCGATGGGCCAGTCCAGTAACCCGGAAGGGGGagtg                                                                                                                                                                                                                >  1:106695/1‑248 (MQ=255)                                   aTTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCTCACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCt                                                                                                                                                                                                           <  2:94504/251‑1 (MQ=255)                                              aCCGGACGGTGCGATCCGGATTTTCTCCGCCCACGCGGTTTCTCAGGCGGTCCGTAACGAAGCCAAAAGCCGCGATGTGACGGTGTTCGATGCCACCTGTCTGGTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCAGTGGTGTCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAGCCGGAAGTGGAAGGTACGATGGGCAAGTACAGTAACACGGAAGGGTGAATGTATCTGGGCGAATCg                                                                                                                                                                                                 <  2:224374/250‑1 (MQ=255)                                                    cGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTGCGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGGATCTGCTCGATTCGCCGGACg                                                                                                                                                                                          <  2:41216/251‑1 (MQ=255)                                                         cgATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGCCCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGCAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAtgtg                                                                                                                                                                                     >  2:107199/1‑251 (MQ=255)                                                             ccTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTgg                                                                                                                                                                                  <  1:188220/250‑1 (MQ=255)                                                                        ccGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACg                                                                                                                                                                         <  1:170424/248‑1 (MQ=255)                                                                        ccGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTc                                                                                                                                                                      <  1:22910/251‑1 (MQ=255)                                                                        ccGCACACGGTGTTTCTCAGGCGGGACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACg                                                                                                                                                                         <  2:61761/248‑1 (MQ=255)                                                                                   gtTTCTCAGGCGGTACGTAACGAAGAAAAAAGCGGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGGCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCAGCGTCCACGCCGGGCATCCGGAAGTGGAAGGTTCGAGGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCGGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAag                                                                                                                                                            <  1:167701/250‑1 (MQ=255)                                                                                              ggTACGTAAAGAAGCAAAAAGCCGCGATTGGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCt                                                                                                                                                <  2:215648/251‑1 (MQ=255)                                                                                               gTACGTAACGAAGCAAAAAGCCGCGAGTTGACGGTGTTGGATGCCACCTGTCCGCTGGTGACCAAAGGGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGTGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGTAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCtt                                                                                                                                               <  2:269527/251‑1 (MQ=255)                                                                                                 aCGTAACGAAGCAAAAAGCCGCTATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTGGATTTTCATCTGCCACGCCGGGCATCCGGAAGTGGGAGGTACGATGGGCCAGTACAGTAACCCTGAGGGGGGAATGTATCTGGTCGAATCGCCGTAGGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCttt                                                                                                                                              <  1:238999/250‑1 (MQ=255)                                                                                                 aCGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCAGCGGCCACGCCGGGCATCCGGAAGTGGAATGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTa                                                                                                                                             <  1:237567/251‑1 (MQ=255)                                                                                                     aaCGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGa                                                                                                                                          <  1:2347/250‑1 (MQ=255)                                                                                                                          ttGACTGTGGTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGAGTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCTGACGATGTGTGGAAACTAACGGTCAAAAACGAAGATAAGCTCTCTGTTAGGATCCAGGCCTCGTTGTCGGTgg                                                                                                                     <  2:139101/250‑1 (MQ=255)                                                                                                                                          ccACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGATCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAtg                                                                                                      >  2:232596/1‑249 (MQ=255)                                                                                                                                                          gACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCTAAGAGTCGATTCTCATCGGCCACGCCGGGCAGCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAACCGGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTa                                                                                    <  2:106425/251‑1 (MQ=255)                                                                                                                                                          gACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCTGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTa                                                                                    <  2:8658/251‑1 (MQ=255)                                                                                                                                                          gACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAGGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGt                                                                                     >  2:207589/1‑250 (MQ=255)                                                                                                                                                           aCCAAAGTGCATATGGAAGTCGCCCGAGCCCGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCCTCCGGAAGTGGAAGGAACGATGGGCCAGTACAGTAACACGGAAGGGGGAATGTATCTGGACGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTATCCTTTATGACCCAGCCCACGCTGTCGGGGGATGACACGTCTGATGTGATCGACGCGCTgagca                                                                                    >  1:182533/1‑246 (MQ=255)                                                                                                                                                                aGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGCGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATACGGCAGTGGAAGGTACGATGGGCCAGTACAGTACCCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGACGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCt                                                                              >  2:129682/1‑251 (MQ=255)                                                                                                                                                                           aaGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGGCACGTCTGATGTGATCGACGCGCGGCGTAAACGCTTCCCGAACAtt                                                                   >  2:188627/1‑251 (MQ=255)                                                                                                                                                                                               cGTGGCGAAGAGTCGATTCTCATAGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTCCAGTAACCCGGAAGGGGGAATGTATCTGGTCGACTCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAAAGCTTCCCGCAAATTGTCGGTCCGCGCAAAGagg                                                >  2:95101/1‑248 (MQ=255)                                                                                                                                                                                                     gaagagTCGATTCTCATCGGCAACGCCGGGCATCCGGACGTGGAAGGTACGATGGGCCAGTACAGTAACACGGCAGGGGGAATGAGTCTGGTCGAATGGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTATCCTTTATGACCCAGACCACGGTGTCGGTGGATGACACGTCTGATGAGATCGACGCGCTGCGTCAACGCTTCCCGGAAATTGTCGGGCCGCGCAAAGATACCATCTg                                         >  2:266270/1‑251 (MQ=255)                                                                                                                                                                                                                                  gcatccGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTAGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACTACTAACCGTCAGGAAGCgg            <  1:227976/247‑1 (MQ=255)                                                                                                                                                                                                                                      ccGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTTATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCATGAAGCGGTAcg        <  2:261936/251‑1 (MQ=255)                                                                                                                                                                                                                                            gTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGAGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGACACGACTAACCGTCAGGAAGCGGTACGCGCCCt  >  1:45615/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   CTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCT  >  NC_000913/26420‑26905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑