Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 26,648 C→T H124H (CAC→CAT)  ispH → 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091326,6480CT93.8% 76.1 / NA 32H124H (CAC→CAT) ispH4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (13/17);  minor base G (0/2);  total (13/19)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTG  >  NC_000913/26407‑26866
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                          
aTGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTg                                                                                                                                                                                                                   <  2:21587/251‑1 (MQ=255)
    ggTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGACGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGCAGTGGa                                                                                                                                                                                                                 >  2:66492/1‑249 (MQ=255)
             cTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATACGGCAGTGGAAGGTACGATgg                                                                                                                                                                                                      >  1:215648/1‑251 (MQ=255)
              ttGCGTGAGCGTGTGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGCCGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCAGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGGACGATggg                                                                                                                                                                                                     >  1:269527/1‑251 (MQ=255)
              ttGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTACTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGAGGAAGGTACGATggg                                                                                                                                                                                                     >  1:264985/1‑251 (MQ=255)
               tGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGGAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATGCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATggg                                                                                                                                                                                                     <  1:122352/250‑1 (MQ=255)
                            ggCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAAcc                                                                                                                                                                                       <  1:50281/251‑1 (MQ=255)
                                          aGCAGATTAGCGAAGTACCGGAGGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAACGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGTAGGTCCGATGGGCCAGTCCAGTAACCCGGAAGGGGGagtg                                                                                                                                                                         >  1:106695/1‑248 (MQ=255)
                                               aTTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCTCACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCt                                                                                                                                                                    <  2:94504/251‑1 (MQ=255)
                                                          aCCGGACGGTGCGATCCGGATTTTCTCCGCCCACGCGGTTTCTCAGGCGGTCCGTAACGAAGCCAAAAGCCGCGATGTGACGGTGTTCGATGCCACCTGTCTGGTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCAGTGGTGTCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAGCCGGAAGTGGAAGGTACGATGGGCAAGTACAGTAACACGGAAGGGTGAATGTATCTGGGCGAATCg                                                                                                                                                          <  2:224374/250‑1 (MQ=255)
                                                                cGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTGCGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGGATCTGCTCGATTCGCCGGACg                                                                                                                                                   <  2:41216/251‑1 (MQ=255)
                                                                     cgATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGCCCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGCAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAtgtg                                                                                                                                              >  2:107199/1‑251 (MQ=255)
                                                                         ccTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTgg                                                                                                                                           <  1:188220/250‑1 (MQ=255)
                                                                                    ccGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACg                                                                                                                                  <  1:170424/248‑1 (MQ=255)
                                                                                    ccGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTc                                                                                                                               <  1:22910/251‑1 (MQ=255)
                                                                                    ccGCACACGGTGTTTCTCAGGCGGGACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACg                                                                                                                                  <  2:61761/248‑1 (MQ=255)
                                                                                               gtTTCTCAGGCGGTACGTAACGAAGAAAAAAGCGGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGGCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCAGCGTCCACGCCGGGCATCCGGAAGTGGAAGGTTCGAGGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCGGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAag                                                                                                                     <  1:167701/250‑1 (MQ=255)
                                                                                                          ggTACGTAAAGAAGCAAAAAGCCGCGATTGGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCt                                                                                                         <  2:215648/251‑1 (MQ=255)
                                                                                                           gTACGTAACGAAGCAAAAAGCCGCGAGTTGACGGTGTTGGATGCCACCTGTCCGCTGGTGACCAAAGGGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGTGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGTAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCtt                                                                                                        <  2:269527/251‑1 (MQ=255)
                                                                                                             aCGTAACGAAGCAAAAAGCCGCTATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTGGATTTTCATCTGCCACGCCGGGCATCCGGAAGTGGGAGGTACGATGGGCCAGTACAGTAACCCTGAGGGGGGAATGTATCTGGTCGAATCGCCGTAGGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCttt                                                                                                       <  1:238999/250‑1 (MQ=255)
                                                                                                             aCGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCAGCGGCCACGCCGGGCATCCGGAAGTGGAATGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTa                                                                                                      <  1:237567/251‑1 (MQ=255)
                                                                                                                 aaCGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGa                                                                                                   <  1:2347/250‑1 (MQ=255)
                                                                                                                                      ttGACTGTGGTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGAGTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCTGACGATGTGTGGAAACTAACGGTCAAAAACGAAGATAAGCTCTCTGTTAGGATCCAGGCCTCGTTGTCGGTgg                                                                              <  2:139101/250‑1 (MQ=255)
                                                                                                                                                      ccACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGATCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAtg                                                               >  2:232596/1‑249 (MQ=255)
                                                                                                                                                                      gACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCTAAGAGTCGATTCTCATCGGCCACGCCGGGCAGCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAACCGGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTa                                             <  2:106425/251‑1 (MQ=255)
                                                                                                                                                                      gACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCTGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTa                                             <  2:8658/251‑1 (MQ=255)
                                                                                                                                                                      gACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAGGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGt                                              >  2:207589/1‑250 (MQ=255)
                                                                                                                                                                       aCCAAAGTGCATATGGAAGTCGCCCGAGCCCGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCCTCCGGAAGTGGAAGGAACGATGGGCCAGTACAGTAACACGGAAGGGGGAATGTATCTGGACGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTATCCTTTATGACCCAGCCCACGCTGTCGGGGGATGACACGTCTGATGTGATCGACGCGCTgagca                                             >  1:182533/1‑246 (MQ=255)
                                                                                                                                                                            aGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGCGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATACGGCAGTGGAAGGTACGATGGGCCAGTACAGTACCCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGACGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCt                                       >  2:129682/1‑251 (MQ=255)
                                                                                                                                                                                       aaGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGGCACGTCTGATGTGATCGACGCGCGGCGTAAACGCTTCCCGAACAtt                            >  2:188627/1‑251 (MQ=255)
                                                                                                                                                                                                           cGTGGCGAAGAGTCGATTCTCATAGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTCCAGTAACCCGGAAGGGGGAATGTATCTGGTCGACTCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAAAGCTTCCCGCAAATTGTCGGTCCGCGCAAAGagg         >  2:95101/1‑248 (MQ=255)
                                                                                                                                                                                                                 gaagagTCGATTCTCATCGGCAACGCCGGGCATCCGGACGTGGAAGGTACGATGGGCCAGTACAGTAACACGGCAGGGGGAATGAGTCTGGTCGAATGGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTATCCTTTATGACCCAGACCACGGTGTCGGTGGATGACACGTCTGATGAGATCGACGCGCTGCGTCAACGCTTCCCGGAAATTGTCGGGCCGCGCAAAGATACCATCTg  >  2:266270/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                          
ATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTG  >  NC_000913/26407‑26866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑