Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,685	G→A	R160K (AGA→AAA)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,685	0	G	A	93.3%	85.0 / NA	30	R160K (AGA→AAA)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/14);  minor base C (2/0);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

AGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTA  >  NC_000913/25444‑25922                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              aGCGTGATCCTGGCAGTGCTGATGTATAGGTCGACGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGCTCGCCTTCTACGTCGGCGGCTGGCACTTCGGCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGCAGGTTTTTTACCTTCTAAAGCGaaaa                                                                                                                                                                                                                                       >  2:181216/1‑250 (MQ=255)    gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCAAAACAATTTCGTTTACGCGCTGTTTATGGGCGGCGCGCTGGGCAACCTGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACa                                                                                                                                                                                                                                   <  1:190592/251‑1 (MQ=255)      gATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaat                                                                                                                                                                                                                                 >  1:216340/1‑251 (MQ=255)        cccTGGCAGTGATGATTTATCTCTCGAAGCCCACTCAGAAGCTAACCAATATCGCTTACGCGTTTATTATTGGCGGCTCGCTGGTCCACCTGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaataa                                                                                                                                                                                                                               <  2:90266/250‑1 (MQ=255)                  tatgcgatATCGCCCGAAGTCCTCGCAGAAGCTAACCAATTTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGCCAACCTCTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGCCTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTTTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTg                                                                                                                                                                                                                      <  1:116991/243‑1 (MQ=255)                        gTATCGCTCGAAGGCCACGCAGAAGCTAAACAATTTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatg                                                                                                                                                                                                               <  1:104199/251‑1 (MQ=255)                              cTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatgc                                                                                                                                                                                                         >  1:166529/1‑251 (MQ=255)                                         cGCAGAAGCTAAACATTATCTCTTACGCGTCGTTTATTGACGGCGCGCAGGGCAACTTGTTCGACCGCCTGTGGCACGGTTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                                                                                                              <  1:6859/251‑1 (MQ=255)                                          gCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                                                                                                              >  2:222700/1‑250 (MQ=255)                                                           tCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtg                                                                                                                                                                             <  1:57340/250‑1 (MQ=255)                                                             gCTTACGCGCTGATTATTGGCGGCGCGCTGG‑CAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGCTATGATCGACTTCTACGTCCGCGGCCGGCCCTTAGCCACCTTCAACCTTGCCGATACTGCCATCTGGGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAACCTGCCGGATGCGCTGCTGACGCATCTTATCCGGCCTACAGATtgctgg                                                                                                                                                                         >  1:117649/1‑250 (MQ=255)                                                               ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCg                                                                                                                                                                        >  1:82636/1‑251 (MQ=255)                                                                                gcggcgCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAATCCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATa                                                                                                                                                       <  1:33903/251‑1 (MQ=255)                                                                                          gggCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGCTTGTGCTGGAAGGTTTTTTACCTTCTACAGCGAAAAAACAATAATAAACCCTGCCGGCTGCGATCCTGACGCATCTTATCCGGCCTACAGATTGCTGCGCAATCGTAGGCCGGCTCAGACGTTTAc                                                                                                                                             >  1:54679/1‑251 (MQ=255)                                                                                          gggCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTCAGCCGGATAAGGCGTTTAc                                                                                                                                             >  1:6246/1‑251 (MQ=255)                                                                                                     tCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                  >  1:86778/1‑251 (MQ=255)                                                                                                       gACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGCTAAGGCGTTTACGCCGCATCCGGc                                                                                                                                 >  1:228373/1‑250 (MQ=255)                                                                                                                 ggCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa                                                                                                                      <  1:97566/251‑1 (MQ=255)                                                                                                                  gCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCCCTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa                                                                                                                      >  2:191885/1‑250 (MQ=255)                                                                                                                                           gACTTCTACGTCGGCGGCTGGCACTTCGCCCCCTTCAACCTTGCCGACACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTGAAGCGAAAAAACAATAATAGACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGCGCTCGCAGGCCGGATAAGTCGCTTACGCCGCATCAGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGtttg                                                                                            >  2:7734/1‑248 (MQ=255)                                                                                                                                           gACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTg                                                                                            <  1:191885/251‑1 (MQ=255)                                                                                                                                                  aCGTCGGCGGCTGGCTCTTCGGCACCTTCAACCTTGCCGATAGTGCCATCTGTGGCGGTGCGGCACTGATTGTGTTGGAAGGTTTTTTACCGTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGTCTCATCTGATCCGGCCTTCATATTGCTGCGAAATCTTAGTCCGGATAAGGCGTTTACGCCTCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCt                                                                                       <  1:244753/249‑1 (MQ=255)                                                                                                                                                   cGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGGGCGGCACTGATTGTGCTGGAAGGTTTGTTACCTTCTAAAGCGAAAAAACAAGAAGAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACATATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa                                                                                    <  1:181216/251‑1 (MQ=255)                                                                                                                                                              ggCACTTCTCCACCCTCAACCTTGCCGATACTTCCATCTTTGTCGGTGCGGCACTGATTGTGCTGGAATGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATTCTGACGCATCTTATCCGGCCTACAGATTTCTGCGAAATCTTAGCCCGTATAAGGCGTTTACGCTGCATCCTGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTCTACAGCGCAATa                                                                          <  2:267743/250‑1 (MQ=255)                                                                                                                                                                                           aCTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAAc                                            <  2:72482/251‑1 (MQ=255)                                                                                                                                                                                            cTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACt                                           >  1:12123/1‑251 (MQ=255)                                                                                                                                                                                                          ggTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGcacc                              >  1:158159/1‑250 (MQ=255)                                                                                                                                                                                                               ggCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCCccgcc                        >  2:229998/1‑251 (MQ=255)                                                                                                                                                                                                                                    tttttgaacTTCTACAGCGAAAAAACAATAATAAACCCTGCAGTTTGCGATGCTGACGCATATTATCCGACATACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCAACAATACTTAACTATAAGAGCAAACCTGAATGTCTGAATCTGGACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTACAACTAGACGATGGCACAACCGCCGAGTCGACCCGCACCAACGGt   >  2:177152/9‑251 (MQ=255)                                                                                                                                                                                                                                      ttttACCTTCAAAAGCGAAAAAAAAATAATAACCCCTGCCGGAGGCGTTGCTGACGCATCTTACCCGGCCTACAGATTGCTGCGAAATCGTAGGCGGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCGGTACAGAGCAATAGCGCCGTCCTGGTGCCCTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTa  <  1:153762/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              AGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTA  >  NC_000913/25444‑25922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑