Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,677	G→A	L157L (TTG→TTA)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,677	0	G	A	100.0%	89.8 / NA	32	L157L (TTG→TTA)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

TTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTA  >  NC_000913/25436‑25922                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ttGGTATTAGCGTCATCCTGGCAGTGATGCCGTCTCGATAGCAGGACCCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAAACTGTTCGACCGCCTGTGGCCCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCCACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaa                                                                                                                                                                                                                                               >  2:42796/1‑249 (MQ=255)  tgttATTAGCGTGACCCTGGCAGTAATGATGTATCGCGCTAAGGCCACGCAGAAGCTAAACAATGTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCGACCTGTGCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTTCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaaa                                                                                                                                                                                                                                              <  1:38096/247‑3 (MQ=255)  tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTCCGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaa                                                                                                                                                                                                                                               >  1:72482/1‑248 (MQ=255)         aGCGTGATCCTGGCAGTGCTGATGTATAGGTCGACGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGCTCGCCTTCTACGTCGGCGGCTGGCACTTCGGCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGCAGGTTTTTTACCTTCTAAAGCGaaaa                                                                                                                                                                                                                                       >  2:181216/1‑250 (MQ=255)            gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCAAAACAATTTCGTTTACGCGCTGTTTATGGGCGGCGCGCTGGGCAACCTGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACa                                                                                                                                                                                                                                   <  1:190592/251‑1 (MQ=255)              gATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaat                                                                                                                                                                                                                                 >  1:216340/1‑251 (MQ=255)                cccTGGCAGTGATGATTTATCTCTCGAAGCCCACTCAGAAGCTAACCAATATCGCTTACGCGTTTATTATTGGCGGCTCGCTGGTCCACCTGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaataa                                                                                                                                                                                                                               <  2:90266/250‑1 (MQ=255)                          tatgcgatATCGCCCGAAGTCCTCGCAGAAGCTAACCAATTTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGCCAACCTCTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGCCTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTTTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTg                                                                                                                                                                                                                      <  1:116991/243‑1 (MQ=255)                                gTATCGCTCGAAGGCCACGCAGAAGCTAAACAATTTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatg                                                                                                                                                                                                               <  1:104199/251‑1 (MQ=255)                                      cTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatgc                                                                                                                                                                                                         >  1:166529/1‑251 (MQ=255)                                                 cGCAGAAGCTAAACATTATCTCTTACGCGTCGTTTATTGACGGCGCGCAGGGCAACTTGTTCGACCGCCTGTGGCACGGTTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                                                                                                              <  1:6859/251‑1 (MQ=255)                                                  gCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                                                                                                              >  2:222700/1‑250 (MQ=255)                                                                   tCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtg                                                                                                                                                                             <  1:57340/250‑1 (MQ=255)                                                                     gCTTACGCGCTGATTATTGGCGGCGCGCTGG‑CAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGCTATGATCGACTTCTACGTCCGCGGCCGGCCCTTAGCCACCTTCAACCTTGCCGATACTGCCATCTGGGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAACCTGCCGGATGCGCTGCTGACGCATCTTATCCGGCCTACAGATtgctgg                                                                                                                                                                         >  1:117649/1‑250 (MQ=255)                                                                       ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCg                                                                                                                                                                        >  1:82636/1‑251 (MQ=255)                                                                                        gcggcgCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAATCCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATa                                                                                                                                                       <  1:33903/251‑1 (MQ=255)                                                                                                  gggCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGCTTGTGCTGGAAGGTTTTTTACCTTCTACAGCGAAAAAACAATAATAAACCCTGCCGGCTGCGATCCTGACGCATCTTATCCGGCCTACAGATTGCTGCGCAATCGTAGGCCGGCTCAGACGTTTAc                                                                                                                                             >  1:54679/1‑251 (MQ=255)                                                                                                  gggCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTCAGCCGGATAAGGCGTTTAc                                                                                                                                             >  1:6246/1‑251 (MQ=255)                                                                                                             tCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                  >  1:86778/1‑251 (MQ=255)                                                                                                               gACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGCTAAGGCGTTTACGCCGCATCCGGc                                                                                                                                 >  1:228373/1‑250 (MQ=255)                                                                                                                         ggCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa                                                                                                                      <  1:97566/251‑1 (MQ=255)                                                                                                                          gCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCCCTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa                                                                                                                      >  2:191885/1‑250 (MQ=255)                                                                                                                                                   gACTTCTACGTCGGCGGCTGGCACTTCGCCCCCTTCAACCTTGCCGACACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTGAAGCGAAAAAACAATAATAGACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGCGCTCGCAGGCCGGATAAGTCGCTTACGCCGCATCAGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGtttg                                                                                            >  2:7734/1‑248 (MQ=255)                                                                                                                                                   gACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTg                                                                                            <  1:191885/251‑1 (MQ=255)                                                                                                                                                          aCGTCGGCGGCTGGCTCTTCGGCACCTTCAACCTTGCCGATAGTGCCATCTGTGGCGGTGCGGCACTGATTGTGTTGGAAGGTTTTTTACCGTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGTCTCATCTGATCCGGCCTTCATATTGCTGCGAAATCTTAGTCCGGATAAGGCGTTTACGCCTCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCt                                                                                       <  1:244753/249‑1 (MQ=255)                                                                                                                                                           cGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGGGCGGCACTGATTGTGCTGGAAGGTTTGTTACCTTCTAAAGCGAAAAAACAAGAAGAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACATATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa                                                                                    <  1:181216/251‑1 (MQ=255)                                                                                                                                                                      ggCACTTCTCCACCCTCAACCTTGCCGATACTTCCATCTTTGTCGGTGCGGCACTGATTGTGCTGGAATGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATTCTGACGCATCTTATCCGGCCTACAGATTTCTGCGAAATCTTAGCCCGTATAAGGCGTTTACGCTGCATCCTGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTCTACAGCGCAATa                                                                          <  2:267743/250‑1 (MQ=255)                                                                                                                                                                                                   aCTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAAc                                            <  2:72482/251‑1 (MQ=255)                                                                                                                                                                                                    cTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACt                                           >  1:12123/1‑251 (MQ=255)                                                                                                                                                                                                                  ggTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGcacc                              >  1:158159/1‑250 (MQ=255)                                                                                                                                                                                                                       ggCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCCccgcc                        >  2:229998/1‑251 (MQ=255)                                                                                                                                                                                                                                              ttttACCTTCAAAAGCGAAAAAAAAATAATAACCCCTGCCGGAGGCGTTGCTGACGCATCTTACCCGGCCTACAGATTGCTGCGAAATCGTAGGCGGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCGGTACAGAGCAATAGCGCCGTCCTGGTGCCCTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTa  <  1:153762/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      TTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTA  >  NC_000913/25436‑25922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑