| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 25,598 | A→G | D131G (GAC→GGC) | lspA → | lipoprotein signal peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 25,598 | 0 | A | G | 96.8% | 84.5 / ‑6.1 | 31 | D131G (GAC→GGC) | lspA | lipoprotein signal peptidase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (15/15); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAACTATGGCGCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTA > NC_000913/25362‑25840 | aaaCTATGGCGCGGGGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGTTATTAGCGTGACCCGGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCa < 1:41764/250‑1 (MQ=255) caCTAAGGCCCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGTCACCGTTGGGTCTTTTCCGGTATTGCGCCTGGCATTATGGCGATCCTGCCAGGCATGCTGTATCAGTCGCAGGCCACGCAGAAGCTAAACAATATCGCTTTCGCGCCGATTATTGCCGGTGCGCCGGGCACCCTGTTCCACCGCCTTGGGCCAGGCTTCGGTGCCGGTATGATCGCCTTCTACGTCGGAGACGTGCACttgcccac > 2:124335/2‑245 (MQ=255) aggggcggcTGGCAGCGTTGGTTCTTGGCCGGTATTGCGATTGGTATTAGCTTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCGGTTCGACCGCCTGTGGCACGGCTTCGTTGGCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCg < 1:254206/247‑1 (MQ=255) ggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGACGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCTCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGccgc > 2:153762/1‑248 (MQ=255) tttGCCTGTGTTGCGATTGGTATTAGCGGGTCCCGGGCAGTGATGAGTTATCGCTCGAAGCCCCCGCAAAAGTTAAACAATTCCGCTTACGCCCTTATTATGGGCGGCGCGCTGGGCACCCTGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTTCTGGAAg < 2:179405/250‑1 (MQ=255) ttGGTATTAGCGTCATCCTGGCAGTGATGCCGTCTCGATAGCAGGACCCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAAACTGTTCGACCGCCTGTGGCCCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCCACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaa > 2:42796/1‑249 (MQ=255) tgttATTAGCGTGACCCTGGCAGTAATGATGTATCGCGCTAAGGCCACGCAGAAGCTAAACAATGTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCGACCTGTGCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTTCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaaa < 1:38096/247‑3 (MQ=255) tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTCCGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaa > 1:72482/1‑248 (MQ=255) aGCGTGATCCTGGCAGTGCTGATGTATAGGTCGACGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGCTCGCCTTCTACGTCGGCGGCTGGCACTTCGGCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGCAGGTTTTTTACCTTCTAAAGCGaaaa > 2:181216/1‑250 (MQ=255) gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCAAAACAATTTCGTTTACGCGCTGTTTATGGGCGGCGCGCTGGGCAACCTGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACa < 1:190592/251‑1 (MQ=255) gATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaat > 1:216340/1‑251 (MQ=255) cccTGGCAGTGATGATTTATCTCTCGAAGCCCACTCAGAAGCTAACCAATATCGCTTACGCGTTTATTATTGGCGGCTCGCTGGTCCACCTGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaataa < 2:90266/250‑1 (MQ=255) tatgcgatATCGCCCGAAGTCCTCGCAGAAGCTAACCAATTTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGCCAACCTCTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGCCTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTTTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTg < 1:116991/243‑1 (MQ=255) gTATCGCTCGAAGGCCACGCAGAAGCTAAACAATTTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatg < 1:104199/251‑1 (MQ=255) cTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatgc > 1:166529/1‑251 (MQ=255) cGCAGAAGCTAAACATTATCTCTTACGCGTCGTTTATTGACGGCGCGCAGGGCAACTTGTTCGACCGCCTGTGGCACGGTTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt < 1:6859/251‑1 (MQ=255) gCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt > 2:222700/1‑250 (MQ=255) tCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtg < 1:57340/250‑1 (MQ=255) gCTTACGCGCTGATTATTGGCGGCGCGCTGG‑CAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGCTATGATCGACTTCTACGTCCGCGGCCGGCCCTTAGCCACCTTCAACCTTGCCGATACTGCCATCTGGGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAACCTGCCGGATGCGCTGCTGACGCATCTTATCCGGCCTACAGATtgctgg > 1:117649/1‑250 (MQ=255) ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCg > 1:82636/1‑251 (MQ=255) gcggcgCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAATCCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATa < 1:33903/251‑1 (MQ=255) gggCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGCTTGTGCTGGAAGGTTTTTTACCTTCTACAGCGAAAAAACAATAATAAACCCTGCCGGCTGCGATCCTGACGCATCTTATCCGGCCTACAGATTGCTGCGCAATCGTAGGCCGGCTCAGACGTTTAc > 1:54679/1‑251 (MQ=255) gggCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTCAGCCGGATAAGGCGTTTAc > 1:6246/1‑251 (MQ=255) tCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg > 1:86778/1‑251 (MQ=255) gACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGCTAAGGCGTTTACGCCGCATCCGGc > 1:228373/1‑250 (MQ=255) ggCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa < 1:97566/251‑1 (MQ=255) gCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCCCTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTa > 2:191885/1‑250 (MQ=255) gACTTCTACGTCGGCGGCTGGCACTTCGCCCCCTTCAACCTTGCCGACACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTGAAGCGAAAAAACAATAATAGACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGCGCTCGCAGGCCGGATAAGTCGCTTACGCCGCATCAGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGtttg > 2:7734/1‑248 (MQ=255) gACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTg < 1:191885/251‑1 (MQ=255) aCGTCGGCGGCTGGCTCTTCGGCACCTTCAACCTTGCCGATAGTGCCATCTGTGGCGGTGCGGCACTGATTGTGTTGGAAGGTTTTTTACCGTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGTCTCATCTGATCCGGCCTTCATATTGCTGCGAAATCTTAGTCCGGATAAGGCGTTTACGCCTCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCt < 1:244753/249‑1 (MQ=255) cGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGGGCGGCACTGATTGTGCTGGAAGGTTTGTTACCTTCTAAAGCGAAAAAACAAGAAGAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACATATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa < 1:181216/251‑1 (MQ=255) | TAACTATGGCGCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTA > NC_000913/25362‑25840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |