Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,781	C→T	G797G (GGC→GGT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,781	0	C	T	100.0%	179.7 / NA	59	G797G (GGC→GGT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/33);  total (26/33)

GATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGT‑TGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCT  >  NC_000913/24535‑25024                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     gATGCGCTTCTGCTCCGTTGAGATGGGTTCCTGCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTg                                                                                                                                                                                                                                                   <  2:73181/250‑1 (MQ=255)  aTGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGc                                                                                                                                                                                                                                                 <  1:204329/251‑1 (MQ=255)  aTGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCGCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGc                                                                                                                                                                                                                                                 <  1:184302/251‑1 (MQ=255)    gcgcTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAg                                                                                                                                                                                                                                               <  1:198033/251‑1 (MQ=255)      gcTTCTGCGCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGAc                                                                                                                                                                                                                                             <  2:71555/251‑1 (MQ=255)                      gaTGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACCCCGCCAAAGCGGACAGCGTCGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGGACGAAGGCCTGTTTGGTCTGGCAGACAGTGAATCAATGAACg                                                                                                                                                                                                                             <  1:211706/251‑1 (MQ=255)                      gaTGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCTCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCTCTGGTGCGCTGGAGGGCACCAATCCTCTCCTTCACCGCTGATGATGTGTGGGGCTACCTGCCGGGCGAACGGGAAAAATACGTCTTCACCGGTGATTGGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAATGAACg                                                                                                                                                                                                                             <  2:253903/251‑1 (MQ=255)                          ggTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGc                                                                                                                                                                                                                         >  1:146545/1‑251 (MQ=255)                             tCCTGCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGt                                                                                                                                                                                                                       <  1:115295/250‑1 (MQ=255)                                         gACATTATCACAGACCGACAGTCCACCGCCAAAGCTGACCGCGTGTCGCGTCGTAGCTGCCAGACTGCGCTGTATCCCATCGCAGAAGTGCCGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGCAGTGTGGGGCTACCTGCCGGGCGTACGTGCAAAATACGTCTACACCGGTGAGTGGTACTAAGGCCTGATTGGTCTGGCAGACAGTGTAGAAATGAACGTAGCGTGCTGGGGCGAGCt                                                                                                                                                                                                          >  2:223585/1‑251 (MQ=255)                                         gACATCATCAAAGACGGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCt                                                                                                                                                                                                          >  2:223592/1‑251 (MQ=255)                                          aCATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTg                                                                                                                                                                                                         <  1:253349/251‑1 (MQ=255)                                             tcatcaAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGCTGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTg                                                                                                                                                                                                      >  2:120392/1‑251 (MQ=255)                                              catcaAAGACCGTCCGTACACCGCCAAAGCGTACAGCGTGGCGCGTCGTAGCTTCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGa                                                                                                                                                                                                     <  2:142984/251‑1 (MQ=255)                                                  aaaGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGt                                                                                                                                                                                                 >  2:229491/1‑251 (MQ=255)                                                  aaaGACCGTCAGTAACCCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGt                                                                                                                                                                                                 <  2:223866/251‑1 (MQ=255)                                                       ccGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTg                                                                                                                                                                                            <  1:50979/251‑1 (MQ=255)                                                        cGTCATTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGGGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTgg                                                                                                                                                                                           <  1:20974/251‑1 (MQ=255)                                                             gTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGAGCGTGGCGa                                                                                                                                                                                        >  2:71667/1‑249 (MQ=255)                                                              tACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGt                                                                                                                                                                                     >  1:80817/1‑251 (MQ=255)                                                                    gCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACa                                                                                                                                                                                >  2:77281/1‑250 (MQ=255)                                                                                agcgaggCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGCGCGTGGCGAAGTGAACAAAGTCATTGAGcc                                                                                                                                                                   >  2:102205/6‑250 (MQ=255)                                                                                      ggtcgacgtAGCTGCCAGACTGCGCCGCATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCACTCCTCTCCTGCACCGCAGATGAAGTGTGTGGCTACCTGCCGTGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATTCGTTCTGGGTCGATCTGTTGAAAGTGCGTGGCGAGGTGAACAAAGTCTTTGAGCAAGcgcg                                                                                                                                                             <  2:181359/245‑1 (MQ=255)                                                                                      gcgcGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGcgcg                                                                                                                                                             <  1:121752/251‑1 (MQ=255)                                                                                        gcgTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTg                                                                                                                                                           <  1:215523/251‑1 (MQ=255)                                                                                                    ccAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGTCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAg                                                                                                                                               <  2:71311/251‑1 (MQ=255)                                                                                                       gACTGCGCTGTATCACATCGCAGAAGCGCTGCTTCGCTGGATGTCACCACTCCTCTCATTCACCGCTGATGACGTGTGGGGCTACCTGCCGGGTGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGAATGGTCTGGCAGACAGTGAAGCCATGAACGATGAGTTCTGGGACGAGCGGTTGAAAGTGCGTGGCGAGGTGTAAACAGTCAGTAAGCGAGCGCGTGCCAACAAGAAAgtgg                                                                                                                                            >  2:43672/1‑251 (MQ=255)                                                                                                         cTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGGGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAgtgggg                                                                                                                                          >  1:12022/1‑250 (MQ=255)                                                                                                           gcgcTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAgtgggtg                                                                                                                                         <  1:229491/250‑1 (MQ=255)                                                                                                                  atCACATTGCAGCAGCGCTGGACCGCTGGATGGCCCAAATACTCTCCTTCACAGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGACGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGACCAAAGCCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTg                                                                                                                                 >  2:32988/1‑251 (MQ=255)                                                                                                                          gCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGc                                                                                                                         <  1:229617/251‑1 (MQ=255)                                                                                                                             gAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGt                                                                                                                      <  2:146545/251‑1 (MQ=255)                                                                                                                                 cgctgagtagctggcaggCACCACTCCTCTCCTTCACCGCTGATGAGGTGAGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACGGGTGAGAGGAACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACTATGCGTTCTGGGACGAGCTGTTGAAAGTGCGAGGCGACGTGACCAAAGTCATTGAGCAGAAGAGTGCGGCCAAGAAAGGGGGTGGCTCGCAGGAAGCGTCAGTAAcc                                                                                                                  >  2:153530/17‑251 (MQ=255)                                                                                                                                              gATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAAc                                                                                                     >  1:218829/1‑251 (MQ=255)                                                                                                                                              gATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCGGGAAGCGGCAGTAACCTTGTATGCAGAAc                                                                                                     <  2:244312/251‑1 (MQ=255)                                                                                                                                               aTGGCACCAATCCTCTCCTTCACCGCTGATGGAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCTCTGGAAGCGGCAGTAACCTTGTATGCAGAAcc                                                                                                    <  1:230957/251‑1 (MQ=255)                                                                                                                                                      cAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTg                                                                                             <  1:216695/251‑1 (MQ=255)                                                                                                                                                          ccTCTCCTTCACAGCTGATGAAGTGAGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCgg                                                                                         >  2:90616/1‑251 (MQ=255)                                                                                                                                                          ccTCGCCTTCACCGCTGAGGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGCAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTTTGCAGAACCGGAACTggcg                                                                                          <  2:83265/250‑4 (MQ=255)                                                                                                                                                                             gaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTgg                                                                       <  1:204278/250‑1 (MQ=255)                                                                                                                                                                                          aCCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACg                                                          >  1:244445/1‑250 (MQ=255)                                                                                                                                                                                                  ggCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTGGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTTCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGTTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTctt                                                 <  2:97374/251‑3 (MQ=255)                                                                                                                                                                                                    cGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTGGTCTTGt                                               <  2:157517/251‑1 (MQ=255)                                                                                                                                                                                                       aCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGa                                           <  1:77281/251‑1 (MQ=255)                                                                                                                                                                                                         gTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGAcc                                         <  1:82548/251‑1 (MQ=255)                                                                                                                                                                                                          tgcaaaaTACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGCTTTGTCTTGT‑TGGCCt                                        >  2:3093/4‑251 (MQ=255)                                                                                                                                                                                                          tGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAACGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGACCt                                        >  2:17340/1‑251 (MQ=255)                                                                                                                                                                                                            aaaaaTACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCCGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTACCCTTGTATGCAGAACCGGAACTGGCGGCGCAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TTACCTcc                                      >  1:130622/1‑251 (MQ=255)                                                                                                                                                                                                              aaaTACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGACCTCCgg                                    >  2:92386/1‑251 (MQ=255)                                                                                                                                                                                                               actACGTCTTCACCGGTGAGTGGGACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGAGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACAGCGCTGGGCGATGAATTACGATTTGTC‑TGTGTGTCCTCCGgc                                   >  2:6859/3‑251 (MQ=255)                                                                                                                                                                                                               aaTACGTCTTCACCGGTGAGTGGTACGAAGGACTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGAGATGAATTACGATTTGTCTTGT‑TGACCTCCGgc                                   >  1:74642/1‑251 (MQ=255)                                                                                                                                                                                                               aaTACGTCTGCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGACCTCCGgc                                   <  2:98410/251‑1 (MQ=255)                                                                                                                                                                                                                 tACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCGGGGCGATGAATTACGATTTGTCTTGT‑TGCCCTCCGgcgc                                 >  1:166630/1‑251 (MQ=255)                                                                                                                                                                                                                       ttCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGACCTCCGGCGCTACCGt                           >  1:78463/1‑251 (MQ=255)                                                                                                                                                                                                                             ggTGAGTGGTACGAAGGCCTGTTTGGTCTGGCCGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGCAAGTGCGCGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGACCTCCGGCGCTACCGTTGc                        >  2:194445/1‑248 (MQ=255)                                                                                                                                                                                                                                        cGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGGCCTCCGGCGCTACCGTTGGAGAGTATAacgacg          <  2:128181/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCAc       <  2:218829/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGT‑TGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctg    <  1:120392/251‑1 (MQ=255)                                                                                                                                                                                                                                                tGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGACGTGAACAAAGTCATTGAGCACGCGCGTGCCGACAAGACAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGCTGAATTACGATTTGTCTTGT‑TGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgct  >  1:35913/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGT‑TGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCT  >  NC_000913/24535‑25024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑