Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,340	T→C	D650D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,340	0	T	C	98.2%	167.1 / NA	54	D650D (GAT→GAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (25/28);  minor base G (0/1);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAC  >  NC_000913/24097‑24577                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              gTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTACCGACAGCTAtc                                                                                                                                                                                                                                        >  2:44650/1‑251 (MQ=255)    cccgtattacctgaTGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTAGATGGTCAGGGACGCACGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGCTGTG‑TTGAACAAACTGGGCGCGGATATTCGGAGTCTGGAGGGGGCATCAACCGACTACACCGGGGAAATGGCCGTTTCTGACGAGATCATGAAACGTGCTGCAGACAGCTAtcgtc                                                                                                                                                                                                                                     >  2:119238/14‑251 (MQ=255)                   gATCTCCACCGCGATGAAAGGCAAAGCGCAGTATCGTAAGGTACTGCCCCCCGGCTTTACAGTGGATGGTCAGGGCCGCAAGACGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGACACGTGCTGCCGACAGCTATCGTCGTATCCGTAACAccc                                                                                                                                                                                                                      >  2:256935/1‑250 (MQ=255)                      ctcCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcgcgc                                                                                                                                                                                                                   >  1:244312/1‑251 (MQ=255)                         cACCGCGATTAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTGCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAGCGTCGTATCCGTAACACCGCGCGCt                                                                                                                                                                                                                <  2:160051/251‑1 (MQ=255)                              cgATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGTTGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctg                                                                                                                                                                                                           <  1:56210/251‑1 (MQ=255)                              cgATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGATATCGTCGTATCCGTAACACCGCGCGCTTCctg                                                                                                                                                                                                           >  2:5514/1‑251 (MQ=255)                               gatgaaaggcacaGCGCCGTATCGTCAGGTACTGCCCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCAATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTCTCCGTCACACCGCGCGCTTCctgg                                                                                                                                                                                                          >  2:104513/13‑250 (MQ=255)                               gATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctgc                                                                                                                                                                                                          <  1:264948/251‑1 (MQ=255)                                  gaaaggcaaaGCGTCGTATCGTCAGGTACTAACCCACGGCTTTACCGTGGATGGTCCGGGCCGCAAGATGTCTAAATCCAGCGGCAACGCCGTTTCTCCGCAGGATGTG‑ATTAACAAACGTGGCGCGGATATTCTTCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTGCTGACGAGATCCTGAAACTTGCTACCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTgg                                                                                                                                                                                                       <  1:114557/244‑1 (MQ=255)                                  gaaaggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctgctg                                                                                                                                                                                                        <  1:4094/243‑1 (MQ=255)                                   aaaggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGc                                                                                                                                                                                                      <  1:113936/245‑1 (MQ=255)                                        caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                                                                                                                                                 >  2:218016/2‑251 (MQ=255)                                        caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                                                                                                                                                 >  2:71156/2‑251 (MQ=255)                                            gcgcCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGaa                                                                                                                                                                                             <  1:49052/251‑1 (MQ=255)                                               ccGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAGCACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGGGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACgg                                                                                                                                                                                          <  1:217003/251‑1 (MQ=255)                                               ccGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACgg                                                                                                                                                                                          >  2:251070/1‑251 (MQ=255)                                                   aTCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGttt                                                                                                                                                                                       <  1:234782/250‑1 (MQ=255)                                                     cGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTTGCAAACCTGAACGGTTTTg                                                                                                                                                                                     <  2:234523/250‑1 (MQ=255)                                                                gACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCaac                                                                                                                                                                           >  2:215523/1‑248 (MQ=255)                                                                gACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCaaa                                                                                                                                                                           >  2:94199/1‑249 (MQ=255)                                                                                gTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCgg                                                                                                                                                          >  2:62264/1‑250 (MQ=255)                                                                                  ggATTGTCAGCGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGGACAAACTGCGCGCGGATATTCTGCGTATGTGGTTGGAATCAACCGACTACACCGGAGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCGATCGCCGTATCCGTAAGACCGCGCGCTTCCTGCTGGCAGACCTGAACGGTTTTGATCCAGCAAAAgctctggtctaacagga                                                                                                                                                         >  2:211706/1‑233 (MQ=255)                                                                                             gCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGGG‑ATGAACAAACTGGGCGCGTATATGCTGCGTCTGTGGGTGGCATCAGCCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCGGAAACGTGCTGCCGACAGCTATCGTCGGATCCGTAACACCGCGCGCTTCCTGGTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAGGTTGGTa                                                                                                                                            <  2:116474/251‑1 (MQ=255)                                                                                                          tAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTggg                                                                                                                               >  1:73975/1‑251 (MQ=255)                                                                                                                    ggCAACACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAGCCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTtg                                                                                                                     <  2:231220/251‑1 (MQ=255)                                                                                                                      caacaCCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTtgtg                                                                                                                   >  1:73181/5‑251 (MQ=255)                                                                                                                          aCCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGa                                                                                                                >  1:191848/1‑250 (MQ=255)                                                                                                                               ttCGCGGCAGGATGTG‑ATGAACAAGCGGGGCGCGGATATTCTGCGACTGTGGGTGTCATCAACCGACTACACCGGTGAAATGGCCGTTCCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGAACCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCTCTGGGCCGTAGGTTGTGCGAAAGcc                                                                                                           <  2:194023/250‑2 (MQ=255)                                                                                                                                 cggcgcAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGc                                                                                                         <  1:182163/247‑1 (MQ=255)                                                                                                                                 cgccgcAGGA‑GTGGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGGGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAATCCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGc                                                                                                         <  2:151262/250‑1 (MQ=255)                                                                                                                                  gccgcAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCATGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGcac                                                                                                       >  1:142984/1‑251 (MQ=255)                                                                                                                                              g‑atgaACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACATCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATcc                                                                                           <  1:253573/251‑1 (MQ=255)                                                                                                                                                 ggaACAAACTGGGCGCGGATAGTCTGCGTCTGTGGGTGGAATCAACCGACTTCACCGGTGAAAGGGCCGTTTCTGACTAGATCCTGAAACGTGCTGCCGAGAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCGGCTGGCAAACCTGAACGGTTTGGATCCAGCAAAAGATATGGGGAAACCGTAAGAGATGGTGTTACTGGATCGCGGGGCCGGAGGTGGTGCGAAAGCGGCATAGGattacattctc                                                                                         <  2:46114/250‑11 (MQ=255)                                                                                                                                                      aaaCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGc                                                                                    <  1:256935/251‑1 (MQ=255)                                                                                                                                                      aaaCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCAGCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGATATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGc                                                                                    <  1:119969/251‑1 (MQ=255)                                                                                                                                                        aCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGt                                                                                  >  1:71555/1‑251 (MQ=255)                                                                                                                                                          tGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTACCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCCAAAGATATGGTGAAACCGGCAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTa                                                                                 >  1:181359/1‑250 (MQ=255)                                                                                                                                                           gggCGCGGATATTCTGCGTCTGTGGGTGGCAGCAACCGACTAAACCGGTGAACTGGCTGTGTCGTACGACATCCTTAAACGGGGTGCCGACAGCTATCGTCGTATCCGTGACACCGCGCGCGTCCTGCTGGCAAACCTGAACGGTGTTGATCCGGGAAAAGATATGGGTAAAGCGGAAGAGATCGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTAc                                                                                <  1:121683/250‑1 (MQ=255)                                                                                                                                                            ggCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGa                                                                              <  1:218016/251‑1 (MQ=255)                                                                                                                                                                            gTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCAc                                                              <  1:210859/251‑1 (MQ=255)                                                                                                                                                                                  gggtggCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTg                                                        <  1:133210/251‑1 (MQ=255)                                                                                                                                                                                                   tACACCGGTGCAATGGCAGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGACGCATACGATTTCCACGAAGTGGTACAGCGTCTGATgcg                                       >  2:121752/1‑251 (MQ=255)                                                                                                                                                                                                    acacCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATgc                                        <  1:157713/249‑1 (MQ=255)                                                                                                                                                                                                          gTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGc                                <  1:210209/251‑1 (MQ=255)                                                                                                                                                                                                            gAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCACACCTGTACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTCGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTAAAGCGTCTGATGCGCTTCTGCTc                              >  2:230957/1‑251 (MQ=255)                                                                                                                                                                                                            gAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTc                              <  2:51964/251‑1 (MQ=255)                                                                                                                                                                                                             aaaTGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTcc                             >  2:253349/1‑251 (MQ=255)                                                                                                                                                                                                             aaaTGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTcc                             >  2:204329/1‑251 (MQ=255)                                                                                                                                                                                                                           gACGAGATCCTGAAACGTTCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGtt                <  2:2645/250‑1 (MQ=255)                                                                                                                                                                                                                               agaTCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACATGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTc           >  1:123695/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCCTGAACCGTGGTGCCGACAGATATCGTCGTATCCGTAACACCGCGCGCTTCCTGATGGCAACCCTGAACGGTTTTGATCCAGCAACAGATATGGTGAAACCGGAGGAGAAGGTGGTACTGGATCGCTGGGCCGTAGGATGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTCCAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTAc        >  2:115295/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTGCCTTCTAc        <  1:71156/250‑1 (MQ=255)                                                                                                                                                                                                                                        aaCGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGATTCCTGCTGGCAAACCTGAACGGTTTTGATACAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCGGGGCCGTAGGGTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAc  <  1:44650/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              GTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTG‑ATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAC  >  NC_000913/24097‑24577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑