Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,116	T→C	Y594H (TAT→CAT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,116	0	T	C	100.0%	53.6 / NA	19	Y594H (TAT→CAT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/11);  total (8/11)

GCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCG  >  NC_000913/1880‑2346                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       gCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGGTGTCACGCCGAACAAAAAGGCCAACAACTCGTCGATGGATTACTACCATCTGTTTCGTCATGCGGCGGaaaa                                                                                                                                                                                                                           >  1:161953/1‑250 (MQ=255)      aaTCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATcgcg                                                                                                                                                                                                                     <  1:1273/251‑1 (MQ=255)           ggAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCCTCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCTCGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCgcgg                                                                                                                                                                                                                    <  1:8281/247‑1 (MQ=255)                   ggCAGGAAGAACTTGCTCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTTTTGACTTCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCGGACTTCTTGCGCGAAGGTTGCCACGTGGTCTCGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAAtt                                                                                                                                                                                                            <  1:43917/247‑1 (MQ=255)                                  cgcAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGGCTTCTTGCGCGCAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGtt                                                                                                                                                                                         >  1:124544/1‑251 (MQ=255)                                              agCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGAtt                                                                                                                                                                              >  1:182701/1‑250 (MQ=255)                                                ccGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTAcc                                                                                                                                                                           <  1:18652/251‑1 (MQ=255)                                                ccGTTTAATCGCGGTGGCTTTATTCGCCTCGTTAAAGAATATCAGCTGCTGAACCGCGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCAGTTCAATAG‑GCCGACTTCGTGCGCGAAGGTGGCCATGTTGTCACGCCGAACAAAAATGTCAACGCCTCGGCGATGGTTTACTTTTATCTGTTTCGTCATGCGGCGGATAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGAGGCTGGATTAcc                                                                                                                                                                           <  1:239620/251‑1 (MQ=255)                                                           ccggCGCTTAATTCGCCTCGTGAAAGAATACCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGGCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGGCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAAGCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGTCTGGATTACCGTTTATTgag                                                                                                                                                                 <  2:126537/248‑1 (MQ=255)                                                           cGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAtt                                                                                                                                                                    <  1:97620/247‑1 (MQ=255)                                                           cGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGGTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTgaga                                                                                                                                                                <  2:171968/251‑1 (MQ=255)                                                                              gTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggg                                                                                                                                                                                      >  1:201534/1‑210 (MQ=255)                                                                              gTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggg                                                                                                                                                                                      <  2:201534/210‑1 (MQ=255)                                                                               tGAAAGAATATCATCTGCTGACCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATCGCGGA‑TTCTTGCGCGAAGGTTTCCACGGTGTCACGCAGAACAAACAGGCCAACACCTCGTCGAAGGATTACTACCATATGTTGCGTCCTGCGGCGGAAAAATCGAGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAACAACTGCTCacg                                                                                                                                            >  2:264836/1‑249 (MQ=255)                                                                                          catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgatg                                                                                                                                  <  1:176866/250‑1 (MQ=255)                                                                                                                                                                  gcgcGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATg                                                           <  1:232900/250‑1 (MQ=255)                                                                                                                                                                              ccACGTTGTCACGCCGAACACAAAGGCCAACCCCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAACTAGCGGCGTAACTTCATCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAgg                                              >  1:97764/1‑251 (MQ=255)                                                                                                                                                                                                                     gATGGATCAATACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACCCCACCGTTGGGGCTGGATTACCGGTTATTGCGCACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCgg       >  2:137905/1‑251 (MQ=255)                                                                                                                                                                                                                          aTTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCcg  >  1:245909/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       GCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCG  >  NC_000913/1880‑2346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑