Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,108	A→T	Q591L (CAG→CTG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,108	0	A	T	100.0%	76.0 / NA	25	Q591L (CAG→CTG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/13);  total (12/13)

TGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCG  >  NC_000913/1862‑2346                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               tGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTg                                                                                                                                                                                                                                            >  1:171968/1‑251 (MQ=255)    tCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCAAACACCTCGTCGATGGATTACTACAATCTGTTGCGt                                                                                                                                                                                                                                         >  1:186638/1‑251 (MQ=255)                   gCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGGTGTCACGCCGAACAAAAAGGCCAACAACTCGTCGATGGATTACTACCATCTGTTTCGTCATGCGGCGGaaaa                                                                                                                                                                                                                           >  1:161953/1‑250 (MQ=255)                        aaTCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATcgcg                                                                                                                                                                                                                     <  1:1273/251‑1 (MQ=255)                             ggAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCCTCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCTCGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCgcgg                                                                                                                                                                                                                    <  1:8281/247‑1 (MQ=255)                                     ggCAGGAAGAACTTGCTCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTTTTGACTTCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCGGACTTCTTGCGCGAAGGTTGCCACGTGGTCTCGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAAtt                                                                                                                                                                                                            <  1:43917/247‑1 (MQ=255)                                                    cgcAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGGCTTCTTGCGCGCAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGtt                                                                                                                                                                                         >  1:124544/1‑251 (MQ=255)                                                                agCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGAtt                                                                                                                                                                              >  1:182701/1‑250 (MQ=255)                                                                  ccGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTAcc                                                                                                                                                                           <  1:18652/251‑1 (MQ=255)                                                                  ccGTTTAATCGCGGTGGCTTTATTCGCCTCGTTAAAGAATATCAGCTGCTGAACCGCGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCAGTTCAATAG‑GCCGACTTCGTGCGCGAAGGTGGCCATGTTGTCACGCCGAACAAAAATGTCAACGCCTCGGCGATGGTTTACTTTTATCTGTTTCGTCATGCGGCGGATAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGAGGCTGGATTAcc                                                                                                                                                                           <  1:239620/251‑1 (MQ=255)                                                                             ccggCGCTTAATTCGCCTCGTGAAAGAATACCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGGCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGGCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAAGCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGTCTGGATTACCGTTTATTgag                                                                                                                                                                 <  2:126537/248‑1 (MQ=255)                                                                             cGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAtt                                                                                                                                                                    <  1:97620/247‑1 (MQ=255)                                                                             cGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGGTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTgaga                                                                                                                                                                <  2:171968/251‑1 (MQ=255)                                                                                             cTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCCATAT‑GCCGTCTTCTTGCGCGAAGGTTTCCACGTTGTCCCGCCGAACAAAAAGGCCAACACCTCGTCGATGGCTTACTACCATCTGtt                                                                                                                                                                                                                                             >  2:123112/1‑158 (MQ=255)                                                                                             cTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt                                                                                                                                                                                                                                             <  1:123112/158‑1 (MQ=255)                                                                                                gTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggg                                                                                                                                                                                      >  1:201534/1‑210 (MQ=255)                                                                                                gTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggg                                                                                                                                                                                      <  2:201534/210‑1 (MQ=255)                                                                                                 tGAAAGAATATCATCTGCTGACCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATCGCGGA‑TTCTTGCGCGAAGGTTTCCACGGTGTCACGCAGAACAAACAGGCCAACACCTCGTCGAAGGATTACTACCATATGTTGCGTCCTGCGGCGGAAAAATCGAGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAACAACTGCTCacg                                                                                                                                            >  2:264836/1‑249 (MQ=255)                                                                                                            catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgatg                                                                                                                                  <  1:176866/250‑1 (MQ=255)                                                                                                                              aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCCCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt                                                                                                                                                                                                                                             >  2:234208/1‑125 (MQ=255)                                                                                                                              aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt                                                                                                                                                                                                                                             <  1:234208/125‑1 (MQ=255)                                                                                                                                                                                    gcgcGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATg                                                           <  1:232900/250‑1 (MQ=255)                                                                                                                                                                                                ccACGTTGTCACGCCGAACACAAAGGCCAACCCCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAACTAGCGGCGTAACTTCATCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAgg                                              >  1:97764/1‑251 (MQ=255)                                                                                                                                                                                                                                       gATGGATCAATACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACCCCACCGTTGGGGCTGGATTACCGGTTATTGCGCACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCgg       >  2:137905/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCcg  >  1:245909/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               TGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCG  >  NC_000913/1862‑2346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑