Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,860	T→C	L490L (CTT→CTC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,860	0	T	C	100.0%	159.5 / NA	51	L490L (CTT→CTC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/26);  total (25/26)

CACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGC  >  NC_000913/23615‑24094                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           cacaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGTGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACt                                                                                                                                                                                                                                       >  1:222082/1‑251 (MQ=255)      aaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATgg                                                                                                                                                                                                                                  <  1:90370/251‑1 (MQ=255)             atcataTTCCGCGCGGCGCCGCAGTGGTTCGTTAGCATGACTCAGCAAGGTCTGCGAGCGCAGTCCCTGCAAGAGATCAAAGGCGGGCAGTGGATCCCGGACTGGGGCCAGGCGCGTCTCGAGTCGATGGTTGATAACCGTCCTGACTGATGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGgaagcagg                                                                                                                                                                                                                           >  2:164704/1‑247 (MQ=255)                  cTTCCGCGCGACGCCGCAGTGGTCCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGCGATCAAAGGCGTGCAGTGGATACCGGACTGGGGCCAGGCGCGTATAGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGACGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGGGGCa                                                                                                                                                                                                                       >  2:172660/1‑250 (MQ=255)                         gcgACGCCGCAGTGGCTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGCCTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCCTCCGCGTACCCTCGACCTGATGGAAGAAGTGGCAAAACCCGt                                                                                                                                                                                                               >  2:133514/1‑251 (MQ=255)                            aCGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGAAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTAGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTg                                                                                                                                                                                                             >  1:110523/1‑250 (MQ=255)                             cgccgcAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGAGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGGGGCAAAACGCGTTGa                                                                                                                                                                                                            >  1:2645/1‑250 (MQ=255)                              gccgcAGCGGTCCATCAGCACGGATCAAAAAGGTCCGCGTGAGCAGTCACTGAAAGAGATCAAAGGCGCGCAGTGGATCCCGGACTGGGGCCAGGCGCGAATCGAGTCGATGCTTACTACACGTCCTGACTGACGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCCCTGTTAGTGCACAAAGACACGGAAGAGCTGCATCCGCGGACCCTCGAACTGATGGAAGAAGGGGCAAAACGCGTTGa                                                                                                                                                                                                            >  2:264948/1‑249 (MQ=255)                                 gcAGTGGTTCGTCAGCATGGCTCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACCCGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGCGTTGAAGtt                                                                                                                                                                                                        >  1:93196/1‑249 (MQ=255)                                        ttCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGttaacggcat                                                                                                                                                                                                >  1:265884/1‑242 (MQ=255)                                             cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTTAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAgg                                                                                                                                                                                           <  2:7072/251‑1 (MQ=255)                                              aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGc                                                                                                                                                                                          <  2:4186/251‑1 (MQ=255)                                              aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCGCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGc                                                                                                                                                                                          <  1:232770/251‑1 (MQ=255)                                                cATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCgt                                                                                                                                                                                        >  1:61591/1‑251 (MQ=255)                                                           aaGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTGGAAGTTAACGGCATCCAGGCGTGGTGGGAtctc                                                                                                                                                                             <  1:226876/251‑1 (MQ=255)                                                                  gcgtgcgCAGACACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                                                                        <  1:66888/249‑1 (MQ=255)                                                                        gcAGTCACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGTGGCCAGGCGCGTAGCGAGGCGATGGTTGCTAACCGGCCTGACTGGTGTATCTCCCGTCAGCGCACCTGTGGCGTACCGATGTCGCTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGAGGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAgaga                                                                                                                                                                <  1:133514/251‑1 (MQ=255)                                                                          aGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATc                                                                                                                                                              <  1:69134/251‑1 (MQ=255)                                                                              aCTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACGGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGGCTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCg                                                                                                                                                          <  1:14075/251‑1 (MQ=255)                                                                                 gAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTGCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGATATCCTCGGc                                                                                                                                                        <  2:246610/250‑1 (MQ=255)                                                                                        aTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGACGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc                                                                                                                                                >  2:241182/1‑251 (MQ=255)                                                                                        aTCAAAGGCGTGCAGTGGAGCCCGGACTGGGGCCAGTGGCGTATCGAGTCGATGGGTGCTAACCGCCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTTACTTGTCGTGCACAAAGACACGGAAGAGCGGTATCCGCGTTCCCTCGAACTGATGTAAGAGGTGGCAAAACGCTTTGAAGTGAACGGCATCCATGCGTGGTGGGAACTCTAGTCGATAGAGATGCTCGGCGACGAAGc                                                                                                                                                <  2:128740/251‑1 (MQ=255)                                                                                           aaaGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTg                                                                                                                                              >  1:63750/1‑250 (MQ=255)                                                                                              ggCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGacc                                                                                                                                           >  1:9880/1‑248 (MQ=255)                                                                                                               ggACTGGGGCCAGGCGCGTATAGAGTCGATGGTTGCTAAACGTCCTGACTGGTGTATATCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGCAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGCTCTCGCTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCgg                                                                                                                         >  2:267054/1‑251 (MQ=255)                                                                                                                        ccAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcg                                                                                                                <  2:50322/251‑9 (MQ=255)                                                                                                                                  aTCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCAGCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGAGCAGTATGTGAAAGTGCTGGatacgctcgacgtatggt                                                                                                       <  2:93196/250‑18 (MQ=255)                                                                                                                                    cGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTg                                                                                                    <  1:163618/251‑1 (MQ=255)                                                                                                                                      agtctgtGGTTGCTAACCGTCCGGACTGGGGTAGCTCCCGGCAGCGCACCTGGGGCGTACCGATGGCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAGGCGAAAGATAGCCTCTGCGACGAAGGTGACCAGTATGTGAAAGCGGCGGATACTCTCGACGTATGGTTTGAc                                                                                                  <  2:256761/245‑1 (MQ=255)                                                                                                                                      aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGAc                                                                                                  <  1:69264/251‑1 (MQ=255)                                                                                                                                             ggTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGAt                                                                                           <  1:243759/251‑1 (MQ=255)                                                                                                                                               ttGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCt                                                                                         >  1:9451/1‑251 (MQ=255)                                                                                                                                                 gCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTAc                                                                                       >  1:56343/1‑251 (MQ=255)                                                                                                                                                   caaCCGTCCTGTCTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTGCATCCGCGTAGCCTCGAACTGATGGAAGAAGTGGCAAAACTCGTTGAAGTTAACGGCATCCAGGCGCGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGTCGTATTGTTTGACTCCGGATCTAccc                                                                                     <  1:134477/250‑1 (MQ=255)                                                                                                                                                       cGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCCctc                                                                                 >  1:158158/1‑251 (MQ=255)                                                                                                                                                            tGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATTTAACTGTTCGTGCACGAAGACACGGAAGAGCTGAATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGGTGTAGTCAACGGCATCCAGGCGTGGGGGGATCTCGCTGCGAAAGAAATCCTCGGCGCTGAAGCTGAACAGTATGGGAAAGAGCCGGATACTCTCGACGGATGGTTTGACTCCGGTTCTTCACACTCTTCtg                                                                            >  2:217003/1‑251 (MQ=255)                                                                                                                                                             gACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACtcttct                                                                             <  1:249312/249‑1 (MQ=255)                                                                                                                                                             gACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                                                                           <  1:218259/251‑1 (MQ=255)                                                                                                                                                                 ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgttg                                                                         <  2:9451/249‑1 (MQ=255)                                                                                                                                                                                  cgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGAATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGACGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGAATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGCAtt                                                      >  1:46114/1‑251 (MQ=255)                                                                                                                                                                                   gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAGGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt                                                     >  2:95156/1‑251 (MQ=255)                                                                                                                                                                                   gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt                                                     <  1:108466/251‑1 (MQ=255)                                                                                                                                                                                                           tGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTAt                             >  1:160051/1‑251 (MQ=255)                                                                                                                                                                                                               cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTg                          <  1:172660/250‑1 (MQ=255)                                                                                                                                                                                                                gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGACTTTGCCGGGCCCGCAGCGGCCATGTATCTgg                         >  2:147828/1‑250 (MQ=255)                                                                                                                                                                                                                           cacGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAcc             <  1:908/251‑1 (MQ=255)                                                                                                                                                                                                                           cacGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAcc             <  1:39286/251‑1 (MQ=255)                                                                                                                                                                                                                            acGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGCCTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACCTGTATCTGGAAGGTTCTGAcc             >  2:157713/1‑250 (MQ=255)                                                                                                                                                                                                                               gAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCTGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCAc                                            <  1:177898/216‑1 (MQ=255)                                                                                                                                                                                                                               gAAGAGCTGAATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGCAGTTACCGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCCCTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCAc                                            >  2:177898/1‑216 (MQ=255)                                                                                                                                                                                                                                      tGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCATCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggt  >  1:174854/1‑247 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           CACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGC  >  NC_000913/23615‑24094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑