Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,803	T→C	G471G (GGT→GGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,803	0	T	C	96.7%	174.1 / NA	60	G471G (GGT→GGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (27/31);  minor base A (2/0);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTT  >  NC_000913/23571‑24043                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 tgcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTc                                                                                                                                                                                                                                >  1:9555/1‑251 (MQ=255)    aCGTTGAGAAAATGCAGCACAGCTATCAGTGCTGCTGACGACACACACCCCCGATCATCTTCCACGCGACGCCGACGCGGTTCGTCAGCATGGCTCAGCAAGGTCCGCGTGCGCCGTCACTGACAGAGATCAAAGGCGTCCCGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTAGCTGGATACTAACCGTCCTGACTGGCGTCTCCCCCGTCAGACCCCCTGGGGAGTACCGACGTCACTGTACGTg                                                                                                                                                                                                                             >  2:118252/1‑251 (MQ=255)      gctGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGTGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGc                                                                                                                                                                                                                            >  2:56210/3‑250 (MQ=255)      gTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCGTCCGCGCGACGCCGCAGGGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGca                                                                                                                                                                                                                           <  2:211471/251‑1 (MQ=255)       gtGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGTTCTGCGGGCGCAGTCACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGTGCCAGGCGCGTATCGAGTCGATGTTTGCTAGCCGTCCTGACTGGTGGACCTCCCGTGAGCGCACGTGAGGCGTACCGATTTCACTGTTCGTGcac                                                                                                                                                                                                                          <  2:166124/250‑1 (MQ=255)        tGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGcac                                                                                                                                                                                                                          >  1:246610/1‑250 (MQ=255)               aTGCAGCACAGCGAGCCGTGCTGCGGGCGTCAGAAAACGTCGATTATCTGCCGTGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTTGATCCCGGACTTGGGCCAGGTGCGTATCGAGTCGATTGTTGCTAACCGTCCTGACTGGGGGATCGCCCGTCAGCTCACCTGGGGCGTACCGATGTCGCTGTTCGTGGACAAAGacac                                                                                                                                                                                                                  <  2:127561/251‑1 (MQ=255)                 gcagcaCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACg                                                                                                                                                                                                                 <  1:234027/250‑1 (MQ=255)                     cacaGCTATCCGTGCTGCTGGCGTCACAAAACGACGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTTGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGa                                                                                                                                                                                                               >  1:80006/1‑248 (MQ=255)                      acaGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAagag                                                                                                                                                                                                           <  1:1992/251‑1 (MQ=255)                         gctgtCCGTTCTGCTGGCGTCACAGAACGGCGATCAGCTTCCGCGCGACGCTGCTGTGGGTCGTTAGTATTGAGCAGAACTGTCTGCGTGCGCAGGCACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGTACTGTGGGCAGGCGCGGATCGTGTCGATGGTTGCTAACCGTCCTTACTGGTGTATCGCCCGTCAGCGCGTCTGCGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTg                                                                                                                                                                                                        <  1:113490/247‑1 (MQ=255)                               cGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCcg                                                                                                                                                                                                  >  1:4186/1‑251 (MQ=255)                                gTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCCCTGTTCGTGCACACAGACACGGAAGAGCTGCATCcgc                                                                                                                                                                                                 >  2:144006/1‑251 (MQ=255)                                 ggctgcgGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGGCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCcgcg                                                                                                                                                                                                <  2:31866/250‑1 (MQ=255)                                  gctgctGGCGTCACAAAACGCCGCTCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGt                                                                                                                                                                                               >  2:232395/1‑251 (MQ=255)                                        ggCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTAccc                                                                                                                                                                                           <  1:160242/249‑1 (MQ=255)                                             cacaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGTGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACt                                                                                                                                                                                    >  1:222082/1‑251 (MQ=255)                                                  aaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATgg                                                                                                                                                                               <  1:90370/251‑1 (MQ=255)                                                         atcataTTCCGCGCGGCGCCGCAGTGGTTCGTTAGCATGACTCAGCAAGGTCTGCGAGCGCAGTCCCTGCAAGAGATCAAAGGCGGGCAGTGGATCCCGGACTGGGGCCAGGCGCGTCTCGAGTCGATGGTTGATAACCGTCCTGACTGATGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGgaagcagg                                                                                                                                                                        >  2:164704/1‑247 (MQ=255)                                                              cTTCCGCGCGACGCCGCAGTGGTCCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGCGATCAAAGGCGTGCAGTGGATACCGGACTGGGGCCAGGCGCGTATAGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGACGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGGGGCa                                                                                                                                                                    >  2:172660/1‑250 (MQ=255)                                                                     gcgACGCCGCAGTGGCTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGCCTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCCTCCGCGTACCCTCGACCTGATGGAAGAAGTGGCAAAACCCGt                                                                                                                                                            >  2:133514/1‑251 (MQ=255)                                                                        aCGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGAAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTAGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTg                                                                                                                                                          >  1:110523/1‑250 (MQ=255)                                                                         cgccgcAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGAGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGGGGCAAAACGCGTTGa                                                                                                                                                         >  1:2645/1‑250 (MQ=255)                                                                          gccgcAGCGGTCCATCAGCACGGATCAAAAAGGTCCGCGTGAGCAGTCACTGAAAGAGATCAAAGGCGCGCAGTGGATCCCGGACTGGGGCCAGGCGCGAATCGAGTCGATGCTTACTACACGTCCTGACTGACGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCCCTGTTAGTGCACAAAGACACGGAAGAGCTGCATCCGCGGACCCTCGAACTGATGGAAGAAGGGGCAAAACGCGTTGa                                                                                                                                                         >  2:264948/1‑249 (MQ=255)                                                                             gcAGTGGTTCGTCAGCATGGCTCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACCCGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGCGTTGAAGtt                                                                                                                                                     >  1:93196/1‑249 (MQ=255)                                                                                    ttCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGttaacggcat                                                                                                                                             >  1:265884/1‑242 (MQ=255)                                                                                      cGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACgg                                                                                                                                                                                                                <  2:211362/182‑1 (MQ=255)                                                                                      cGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACgg                                                                                                                                                                                                                >  1:211362/1‑182 (MQ=255)                                                                                         cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTTAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAgg                                                                                                                                        <  2:7072/251‑1 (MQ=255)                                                                                          aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGc                                                                                                                                       <  2:4186/251‑1 (MQ=255)                                                                                          aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCGCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGc                                                                                                                                       <  1:232770/251‑1 (MQ=255)                                                                                            cATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCgt                                                                                                                                     >  1:61591/1‑251 (MQ=255)                                                                                                       aaGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTGGAAGTTAACGGCATCCAGGCGTGGTGGGAtctc                                                                                                                          <  1:226876/251‑1 (MQ=255)                                                                                                              gcgtgcgCAGACACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                     <  1:66888/249‑1 (MQ=255)                                                                                                                    gcAGTCACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGTGGCCAGGCGCGTAGCGAGGCGATGGTTGCTAACCGGCCTGACTGGTGTATCTCCCGTCAGCGCACCTGTGGCGTACCGATGTCGCTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGAGGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAgaga                                                                                                             <  1:133514/251‑1 (MQ=255)                                                                                                                      aGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATc                                                                                                           <  1:69134/251‑1 (MQ=255)                                                                                                                          aCTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACGGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGGCTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCg                                                                                                       <  1:14075/251‑1 (MQ=255)                                                                                                                             gAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTGCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGATATCCTCGGc                                                                                                     <  2:246610/250‑1 (MQ=255)                                                                                                                                    aTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGACGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc                                                                                             >  2:241182/1‑251 (MQ=255)                                                                                                                                    aTCAAAGGCGTGCAGTGGAGCCCGGACTGGGGCCAGTGGCGTATCGAGTCGATGGGTGCTAACCGCCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTTACTTGTCGTGCACAAAGACACGGAAGAGCGGTATCCGCGTTCCCTCGAACTGATGTAAGAGGTGGCAAAACGCTTTGAAGTGAACGGCATCCATGCGTGGTGGGAACTCTAGTCGATAGAGATGCTCGGCGACGAAGc                                                                                             <  2:128740/251‑1 (MQ=255)                                                                                                                                       aaaGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTg                                                                                           >  1:63750/1‑250 (MQ=255)                                                                                                                                          ggCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGacc                                                                                        >  1:9880/1‑248 (MQ=255)                                                                                                                                                           ggACTGGGGCCAGGCGCGTATAGAGTCGATGGTTGCTAAACGTCCTGACTGGTGTATATCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGCAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGCTCTCGCTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCgg                                                                      >  2:267054/1‑251 (MQ=255)                                                                                                                                                                    ccAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcg                                                             <  2:50322/251‑9 (MQ=255)                                                                                                                                                                              aTCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCAGCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGAGCAGTATGTGAAAGTGCTGGatacgctcgacgtatggt                                                    <  2:93196/250‑18 (MQ=255)                                                                                                                                                                                cGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTg                                                 <  1:163618/251‑1 (MQ=255)                                                                                                                                                                                  agtctgtGGTTGCTAACCGTCCGGACTGGGGTAGCTCCCGGCAGCGCACCTGGGGCGTACCGATGGCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAGGCGAAAGATAGCCTCTGCGACGAAGGTGACCAGTATGTGAAAGCGGCGGATACTCTCGACGTATGGTTTGAc                                               <  2:256761/245‑1 (MQ=255)                                                                                                                                                                                  aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGAc                                               <  1:69264/251‑1 (MQ=255)                                                                                                                                                                                         ggTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGAt                                        <  1:243759/251‑1 (MQ=255)                                                                                                                                                                                           ttGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCt                                      >  1:9451/1‑251 (MQ=255)                                                                                                                                                                                             gCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTAc                                    >  1:56343/1‑251 (MQ=255)                                                                                                                                                                                               caaCCGTCCTGTCTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTGCATCCGCGTAGCCTCGAACTGATGGAAGAAGTGGCAAAACTCGTTGAAGTTAACGGCATCCAGGCGCGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGTCGTATTGTTTGACTCCGGATCTAccc                                  <  1:134477/250‑1 (MQ=255)                                                                                                                                                                                                   cGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCCctc                              >  1:158158/1‑251 (MQ=255)                                                                                                                                                                                                        tGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATTTAACTGTTCGTGCACGAAGACACGGAAGAGCTGAATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGGTGTAGTCAACGGCATCCAGGCGTGGGGGGATCTCGCTGCGAAAGAAATCCTCGGCGCTGAAGCTGAACAGTATGGGAAAGAGCCGGATACTCTCGACGGATGGTTTGACTCCGGTTCTTCACACTCTTCtg                         >  2:217003/1‑251 (MQ=255)                                                                                                                                                                                                         gACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACtcttct                          <  1:249312/249‑1 (MQ=255)                                                                                                                                                                                                         gACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                        <  1:218259/251‑1 (MQ=255)                                                                                                                                                                                                             ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgttg                      <  2:9451/249‑1 (MQ=255)                                                                                                                                                                                                                              cgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGAATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGACGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGAATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGCAtt   >  1:46114/1‑251 (MQ=255)                                                                                                                                                                                                                               gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAGGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt  >  2:95156/1‑251 (MQ=255)                                                                                                                                                                                                                               gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt  <  1:108466/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 TGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTT  >  NC_000913/23571‑24043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑